FPS-ZM1拮抗晚期糖基化终末产物受体逆转高糖所致的牙周膜成纤维细胞炎症反应

doi:10.13701/j.cnki.kqyxyj.2017.11.006

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (11): 1156-1160.DOI: 10.13701/j.cnki.kqyxyj.2017.11.006

FPS-ZM1拮抗晚期糖基化终末产物受体逆转高糖所致的牙周膜成纤维细胞炎症反应

詹聃婷, 丁农乐, 张云水, 张慧, 郭玲^*

西南医科大学附属口腔医院修复科四川泸州 646000

收稿日期:2017-07-11 出版日期:2017-11-20 发布日期:2017-11-29
通讯作者: 郭玲,E-mail:gl2005202@foxmail.com
作者简介:詹聃婷(1992~ ),女,四川泸州人,硕士在读,主要从事口腔修复、口腔种植研究。
基金资助:
四川省科技厅面上项目(编号:2017JY0111)
泸州市-校联合(编号:2016LZXNYD-J20)

FPS-ZM1 Rescues the Inflammatory Response of hPDLFs Caused by High Glucose via Inhibition of RAGE

ZHAN Dan-ting, DING Nong-le, ZHANG Yun-shui, ZHANG Hui, GUO Ling^*

Department of Prosthodontics, Stomatological Hospital of Southwest Medical University, Luzhou 646000, China

Received:2017-07-11 Online:2017-11-20 Published:2017-11-29

摘要/Abstract

摘要： 目的:以体外培养的人牙周膜成纤维细胞(hPDLFs)为研究对象,探讨新型小分子RAGE特异性抑制剂对高糖环境培养下牙周膜成纤维细胞炎性因子的影响。方法:原代培养hPDLFs,Western-Blot检测高糖对RAGE表达的影响,RT-PCR检测FPS-ZM1对高糖环境下IL-6和TNF-α基因表达的影响,ELISA检测IL-6和TNF-α的蛋白分泌变化。结果:高糖可导致hPDLFs表面RAGE表达增加,加重细胞炎症反应。而FPS-ZM1可以下调IL-6和TNF-α的基因表达,同时减少炎症介质IL-6和TNF-α的分泌。结论:RAGE特异性抑制剂FPS-ZM1可以在一定程度上逆转高糖刺激导致hPDLFs产生的炎症反应。

关键词: 牙周膜成纤维细胞, RAGE抑制剂, 高糖, 炎症反应

Abstract: Objective: To observe the effect of FPS-ZM1 on IL-6 and TNF-α expression of human periodontal ligament fibroblasts (hPDLFs) cultured in high glucose. Methods: hPDLFs were primarily cultured and exposed to either low glucose (5.5mmol/L) or high glucose (25mmol/L). Under high glucose, the expression of RAGE was measured by Western-blot. The gene and protein expressions of IL-6 and TNF-α were measured by RT-PCR and ELISA under high glucose with various concentration of FPS-ZM1. Results: High glucose could enhance RAGE expression and the inflammatory response of hPDLFs, which was inverted by RAGE inhibitor FPS-ZM1. Conclusion: FPS-ZM1 can rescue the inflammatory response of hPLDFs caused by high glucose.

Key words: hPDLFs, RAGE inhibitor, High glucose, Inflammatory response

中图分类号:

R781.4

詹聃婷, 丁农乐, 张云水, 张慧, 郭玲. FPS-ZM1拮抗晚期糖基化终末产物受体逆转高糖所致的牙周膜成纤维细胞炎症反应[J]. 口腔医学研究, 2017, 33(11): 1156-1160.

ZHAN Dan-ting, DING Nong-le, ZHANG Yun-shui, ZHANG Hui, GUO Ling. FPS-ZM1 Rescues the Inflammatory Response of hPDLFs Caused by High Glucose via Inhibition of RAGE[J]. Journal of Oral Science Research, 2017, 33(11): 1156-1160.

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FPS-ZM1拮抗晚期糖基化终末产物受体逆转高糖所致的牙周膜成纤维细胞炎症反应

FPS-ZM1 Rescues the Inflammatory Response of hPDLFs Caused by High Glucose via Inhibition of RAGE

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