口腔医学研究

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (11): 1165-1168.DOI: 10.13701/j.cnki.kqyxyj.2017.11.008

• 牙周组织研究 • 上一篇    下一篇

实验性牙周炎模型大鼠牙槽骨微结构的改变

陈景宜1, 张昀1, 陈彩云1, 程岚2, 张修银3*   

  1. 1. 兰州市口腔医院牙周黏膜科 甘肃 兰州 730000;
    2. 上海交通大学医学院附属第九人民医院牙周病科 上海 200011;
    3. 上海交通大学医学院附属第九人民医院口腔修复科,上海市口腔医学重点实验 上海 200011
  • 收稿日期:2017-05-11 出版日期:2017-11-20 发布日期:2017-11-29
  • 通讯作者: 张修银,电话:021-23271699
  • 作者简介:陈景宜(1981~ ),男,甘肃人,学士,主治医师,主要从事牙周病临床及科研工作。
  • 基金资助:
    上海高校高峰高原学科建设项目(编号:20152523)
    兰州口腔临床医学中心(2014)

Changes of Microarchitecture of Alveolar Bone in Experimental Periodontitis in Rats

CHENG Jing-yi1, ZHANG Yun1, CHENG Cai-yun1, CHENG Lan2, ZHANG Xiu-yin3*   

  1. 1. Stomatology Hospital of Lanzhou, Lanzhou 730000, China;
    2. Department of Periodontics, The Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China;
    3. Department of Prosthodontics, The Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology,Shanghai 200011, China
  • Received:2017-05-11 Online:2017-11-20 Published:2017-11-29

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摘要： 目的:研究牙周炎对大鼠根分叉区牙槽骨微结构的影响。方法:3月龄健康未孕大鼠14只,采用丝线结扎大鼠左侧上颌第一磨牙建立实验性牙周炎模型(实验组),右侧作为对照组。分别于建模后4周及8周时处死大鼠,并测量上颌磨牙牙周探诊深度、收集上颌骨标本。采用Micro-CT分析大鼠上颌第一磨牙根中1/3区牙槽骨微结构的改变。结果:4周及8周实验组牙周探针深度均高于对照组(P<0.05).Micro-CT图像分析发现对照组牙槽骨致密,主要由板状骨小梁构成;实验组牙槽骨变疏松,杆状骨小梁较多。牙槽骨微结构参数分析发现4周及8周实验组骨密度(BMD)、骨体积分数(BV/TV)及骨小梁宽度(Tb.Th.)均较对照组降低(P<0.05),且8周实验组BMD、BV/TV、Tb.Th.较4周组降低(P<0.05)。8周实验组骨小梁数目(Tb.N.)及骨髓腔宽度(Tb.Sp.)较对照组及4周实验组均增加(<0.05)。结论:牙周炎可导致大鼠根分叉区牙槽骨丢失、骨小梁微结构破坏,且这种破坏随着时间延长不断加剧。

关键词: 牙周炎, 牙槽骨, Micro-CT, 骨微结构

Abstract: Objective: To study the changes of the microarchitecture of alveolar bone in the experimental periodontitis in rats. Methods: Fourteen 3-month-old Sprague-Dawley rats were used to establish periodontitis model by ligaturing the left maxillary 1st molar (PER), and the right maxillary 1st molar was used as control (CON). The rats were sacrificed respectively 4 weeks and 8 weeks later. The maxilla was analyzed by Micro-CT scanning. All statistical analysis was performed with SPSS. Results: The probing depth in PER group increased in comparison to CON group at both 4 and 8 weeks. The alveolar bone of CON group was compacted with plate-like trabecular, while the alveolar bone of PER group become porotic with rod-like trabecular. When compared with corresponding CON group, BMD, BV/TV, and Tb.Th. of alveolar bone significantly decreased in PER group at both 4 and 8 weeks. BMD, BV/TV, and Tb.Th. in 8-week PER group decreased compared with 4-week PER group. Tb.N and Tb.Sp. in 8-week PER group increased in compared with both CON group and 4-week PER group. Conclusion: Periodontitis could induce bone loss and deterioration of trabeculae of alveolar bone. The deterioration might be aggravated by the extended duration.

Key words: Periodontitis, Alveolar bone, Micro-CT, Microarchitecture

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