口腔白斑病动物模型的研究进展

doi:10.13701/j.cnki.kqyxyj.2025.03.001

摘要/Abstract

摘要： 口腔鳞状细胞癌(oral squamous cell carcinoma, OSCC)患者预后较差,5年生存率不足50%。为了提高生存率和预后,应在口腔潜在恶性疾患(oral potentially malignant disorders, OPMDs)阶段及早进行干预,口腔白斑病(oral leukoplakia, OLK)为最常见OPMDs,建立一个理想的OLK动物模型可以对肿瘤演变的过程有更深刻的认识。本文将对常见的OLK动物模型作一综述。

关键词: 口腔白斑病, 口腔鳞状细胞癌, 动物模型

Abstract: Patients with oral squamous cell carcinoma (OSCC) have a poor prognosis whose 5-year survival rate is less than 50%. Early intervention in the oral potentially malignant disorders (OPMDs) stage is necessary to improve survival rate and prognosis, oral leukoplakia (OLK) is the most common OPMDs. In order to understand the process of OSCC deeply, an ideal OLK animal model is in great request. In this paper, the common animal models of OLK were reviewed.

Key words: oral leukoplakia, oral squamous cell carcinoma, animal models

张琦, 石晶. 口腔白斑病动物模型的研究进展[J]. 口腔医学研究, 2025, 41(3): 179-183.

ZHANG Qi, SHI Jing. Research Progress in Animal Models of Oral Leukoplakia[J]. Journal of Oral Science Research, 2025, 41(3): 179-183.

参考文献

[1] Bray F, Laversanne M, Sung H, et al. Global cancer statistics 2022: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J].CA Cancer J Clin, 2024, 74(3):229-263.
[2] Siegel RL, Miller KD, Wagle NS, et al. Cancer statistics, 2023[J].CA Cancer J Clin, 2023, 73(1): 17-48.
[3] Fan T, Wang X, Zhang S, et al. NUPR1 promotes the proliferation and metastasis of oral squamous cell carcinoma cells by activating TFE3-dependent autophagy[J].Signal Transduct Target Ther, 2022, 7(1):130.
[4] Meci A, Goyal N, Slonimsky G. Mechanisms of resistance and therapeutic perspectives in immunotherapy for advanced head and neck cancers[J].Cancers, 2024,16(4):703.
[5] Hu S, Lu H, Xie W, et al. TDO2⁺ myofibroblasts mediate immune suppression in malignant transformation of squamous cell carcinoma[J].J Clin Invest, 2022, 132(19):e157649.
[6] Guan JY, Luo YH, Lin YY, et al. Malignant transformation rate of oral leukoplakia in the past 20 years: A systematic review and meta-analysis[J].J Oral Pathol Med, 2023, 52(8): 691-700.
[7] Zhang C, Li B, Zeng X, et al. The global prevalence of oral leukoplakia: a systematic review and meta-analysis from 1996 to 2022[J].BMC Oral Health, 2023, 23(1):645.
[8] Flores-Hidalgo A, Phero J, Steward-Tharp S, et al. Immunophenotypic and gene expression analyses of the inflammatory microenvironment in high-grade oral epithelial dysplasia and oral lichen planus[J].Head Neck Pathol, 2024, 18(1):17.
[9] Khayatan D, Hussain A, Tebyaniyan H. Exploring animal models in oral cancer research and clinical intervention: A critical review[J].Vet Med Sci, 2023, 9(4): 1833-1847.
[10] Lee YM, Hsu CL, Chen YH, et al. Genomic and transcriptomic landscape of an oral squamous cell carcinoma mouse model for immunotherapy[J].Cancer Immunol Res, 2023, 11(11): 1553-1567.
[11] Das J, Bera S, Ganguly N, et al. The immunomodulatory impact of naturally derived neem leaf glycoprotein on the initiation progression model of 4NQO induced murine oral carcinogenesis: a preclinical study[J].Front Immunol, 2024, 15:1325161.
[12] Sagheer SH, Whitaker-Menezes D, Han JYS, et al. 4NQO induced carcinogenesis: A mouse model for oral squamous cell carcinoma[J].Methods Cell Biol, 2021, 163:93-111.
[13] Schuch LF, Campagnol D, Schmidt TR, et al. Proposal of a secure and efficient protocol for a murine oral carcinogenesis model induced by 4-nitroquinoline-1-oxide (4NQO)[J].Pathol Res Pract, 2023, 247:154547.
[14] Barcessat ARR, Huang I, Rabelo GD, et al. Systemic toxic effects during early phases of topical 4-NQO-induced oral carcinogenesis in rats[J].J Oral Pathol Med. 2014, 43(10): 770-777.
[15] 李武伟,马国武. 4NQO饮水法诱发小鼠舌癌前病变模型建立的研究[J].口腔医学研究,2005,21(3): 283-285.
[16] 卜冬平,马国武. 两种浓度4NQO饮水法诱发小鼠舌癌及癌前病变动物模型的比较[J].口腔医学研究, 2008, 24(3): 270-273.
[17] Plante I. Dimethylbenz(a)anthracene-induced mammary tumorigenesis in mice[J].Methods Cell Biol, 2021,163:21-44.
[18] 陈梁,周海文. 口腔癌的鼠科动物模型研究进展[J].临床口腔医学杂志,2011,27(08): 508-511.
[19] Ali Syed S, Qureshi MA, Khan S, et al. Development of a murine model of oral carcinogenesis: an accelerated tool for biomarker and anti-tumour drug discovery[J].Ecancermedicalscience, 2022, 16:1413.
[20] 程俊鑫,白贺天,常治楠,等.口腔黏膜癌前病变和口腔癌动物模型的研究进展[J].华西口腔医学杂志,2020,38(2):198-204.
[21] 房栗. 主动吸烟对口腔黏膜上皮细胞NOD1信号通路的影响[D].南京大学, 2016.
[22] 黄菊蕊. 一种改良方法构建小鼠舌癌动物模型以及舌癌转录组测序研究[D].右江民族医学院, 2023.
[23] 徐芳. 局部应用环氧化酶-2抑制剂抑制DMBA诱导的大鼠舌癌作用的研究[D].南方医科大学, 2011.
[24] 张弓长,邓一平,董碧蓉.基因工程小鼠饲养繁育及鉴定策略[J].现代临床医学,2023,49(4):295-298.
[25] Liu C, Wu P, Zhang A, et al. Advances in rodent models for breast cancer formation, progression, and therapeutic testing[J].Front Oncol, 2021, 11:593337.
[26] Salm F, Znalesniak EB, Laskou A, et al. Expression profiling along the murine intestine: different mucosal protection systems and alterations in Tff1-deficient animals[J].Int J Mol Sci, 2023, 24(16):12684.
[27] Wilkey JF, Buchberger G, Saucier K, et al. Cyclin D1 overexpression increases susceptibility to 4nitroquinoline 1-oxide induced dysplasia and neoplasia in murine squamous oral epithelium[J].Mol Carcinog, 2009, 48(9): 853-861.
[28] Quan LL, Liu JY, Qu LX, et al. Expression of Cyclin D1 gene in ovarian cancer and effect of silencing its expression on ovarian cancer cells based on the Oncomine database[J].Bioengineered, 2021, 12(2): 9290-9300.
[29] Opitz OG, Quante M, von Werder A, et al. A mouse model of oral-esophageal carcinogenesis[J].Onkologie, 2005, 28(1): 44-48.
[30] Tasoulas J, Srivastava S, Xu X, et al. Genetically engineered mouse models of head and neck cancers[J].Oncogene, 2023, 42(35): 2593-2609.
[31] Holmstrup P, Dabelsteen E, Roed-Petersen B. Oral leukoplakia transplanted to nude mice[J].Scand J Dent Res, 1981,15(2):275-282.
[32] He F, Zhou X, Huang G, et al. Establishment and identification of patient-derived xenograft model for oral squamous cell carcinoma[J].J Oncol, 2022:3135470.
[33] Ishida K, Tomita H, Nakashima T, et al. Current mouse models of oral squamous cell carcinoma: Genetic and chemically induced models[J].Oral Oncol, 2017, 73: 16-20.
[34] Zohud O, Lone IM, Nashef A, et al. Towards system genetics analysis of head and neck squamous cell carcinoma using the mouse model, cellular platform, and clinical human data[J].Animal Model Exp Med, 2023, 6(6): 537-558.