模拟微重力对人牙髓干细胞在裸鼠体内矿化能力的影响

口腔医学研究 ›› 2015, Vol. 31 ›› Issue (3): 234-236.

模拟微重力对人牙髓干细胞在裸鼠体内矿化能力的影响

徐丹丹, 李艳萍, 潘爽, 张巍巍, 何丽娜, 牛玉梅^*

哈尔滨医科大学口腔医学院牙体牙髓病科黑龙江哈尔滨 150001

收稿日期:2014-09-09 出版日期:2015-03-28 发布日期:2016-04-29
通讯作者: 牛玉梅,E-mail:yumeiniu@163.com
作者简介:徐丹丹(1987~ ),女,黑龙江省绥化人,硕士,医师,主要从事口腔内科的临床治疗工作。
基金资助:
国家自然科学基金(编号:81271132) ;黑龙江省教育厅科学技术研究项目(编号:12531234)

Effect of Simulated Microgravity on Mieralization of Human Dental Pulp Stem Cells on PLGA Scaffolds in Vivo.

XU Dan-dan, LI Yan-ping, PAN Shuang, et al.

Department of Operative Dentistry and Endodontics, School of Stomatology, Harbin Medical University, Harbin 150001

Received:2014-09-09 Online:2015-03-28 Published:2016-04-29

摘要/Abstract

摘要： 目的:研究模拟微重力环境对聚乳酸-羟基乙酸(PLGA)支架上的人牙髓干细胞(human dental pulp stem cells, hDPSCs)在裸鼠体内矿化能力的影响。方法:分离、培养hDPSCs并进行鉴定,然后将hDPSCs接种到PLGA支架上培养72 h后随机分为两组,普通重力组和模拟微重力组,经矿化诱导液培养1周后分别将细胞支架复合物移植到裸鼠体内,植入术后4周取材,进行组织学(HE和Vonkossa)和免疫组织化学(DSPP)检测。结果:HE染色均可见有血管生成,Vonkossa染色均为阳性,模拟微重力组DSPP的表达水平明显高于普通重力组(P<0.05)。结论:模拟微重力有利于hDPSCs在裸鼠体内的矿化。

关键词: 人牙髓干细胞, 旋转细胞培养系统, 支架, 矿化

Abstract: Objective: To investigate the mineralized effect of simulated microgravity on human dental pulp stem cells(hDPSCs) on PLGA(poly lactic-co-glycolic acid) after subcutaneous transplantation into nude mice. Methods: hDPSCs were seeded into PLGA scaffolds and cultured for 72 hours, then cell-scaffold composites were randomly divided into 2 groups: normal gravity group and simulated microgravity group, which were both cultured in osteogenic induction medium. After 1 week, cell-scaffold composites of both groups were respectively transplanted into the subcutaneous tissue of immunodeficiency mice. The samples were retrieved after 4 weeks of implantation for histological(HE and Vonkossa) and immunohistochemical (DSPP) examinations. Results: Angiogenesis was visualized in HE stained sections. Vonkossa staining were positive for assessing mineral deposition. The expression level of DSPP in simulated microgravity group was significant higher than that in normal gravity group(P<0.05). Conclusion: Simulated microgravity is advantageous for the mineralization of hDPSCs in nude mice.

Key words: Human dental pulp stem cells , Rotary cell culture system、 Scaffold, Mieralization

中图分类号:

R780.1

徐丹丹, 李艳萍, 潘爽, 张巍巍, 何丽娜, 牛玉梅. 模拟微重力对人牙髓干细胞在裸鼠体内矿化能力的影响[J]. 口腔医学研究, 2015, 31(3): 234-236.

XU Dan-dan, LI Yan-ping, PAN Shuang, et al.. Effect of Simulated Microgravity on Mieralization of Human Dental Pulp Stem Cells on PLGA Scaffolds in Vivo.[J]. Journal of Oral Science Research, 2015, 31(3): 234-236.

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