牙髓干细胞修复再生同质异体大鼠牙髓牙本质复合体的研究

口腔医学研究 ›› 2015, Vol. 31 ›› Issue (7): 645-648.

牙髓干细胞修复再生同质异体大鼠牙髓牙本质复合体的研究

铁晓敏,刘佳,王丽萍,王璇,李伯琦,刘奕杉^*

新疆医科大学第一附属医院儿牙预防保健科新疆乌鲁木齐 830054

收稿日期:2015-01-05 出版日期:2015-07-28 发布日期:2015-07-28
通讯作者: 刘奕杉,E-mail: lsy-tree@126.com
作者简介:铁晓敏(1989~ )女,汉族,新疆人,硕士,主要从事儿童口腔及预防保健方面的研究。
基金资助:
新疆维吾尔自治区自然科学基金资助项目(编号:2011211A067)

Study on Dental Pulp Stem Cells(DPSCs) Regenerating Homogeneous Rats’ Pulp Dentin Complex

TIE Xiao-min, LIU Jia, WANG Li-ping, et al

Department of Prevention and Health Care of Children’s Teeth,First Affiliated Hospital of Xinjiang Medical University,Urumqi 830054

Received:2015-01-05 Online:2015-07-28 Published:2015-07-28

摘要/Abstract

摘要： 目的:观察第3代同质异体牙髓干细胞(dental pulp stem cell,DPSCs) 定植于冠髓被摘除的大鼠髓腔后,再生修复牙髓牙本质复合体的能力。方法:18只SD大鼠随机分为实验组和对照组,两组均全麻下摘除大鼠上颌双侧第一磨牙冠髓,实验组同期髓腔定植体外培养的同质异体大鼠牙髓干细胞,对照组冠髓摘除后不进行任何处理,术后均使用光固化树脂充填封闭髓腔。两组动物均于术后2、4、6周处死,取其上颌骨进行大体观察,X线观察,组织学观察,牙本质的厚度测量。结果:实验组4周时,肉眼可见牙髓充血减少;6周时,X线显示牙本质厚度增加,根尖孔闭合;6周时,光镜下显示明显新生牙本质形成,成牙本质细胞栅栏状排列;牙本质厚度测量结果显示,实验组牙本质厚度2周时增厚,4周增厚更明显,持续至6周,优于对照组(P<0.05)。结论:牙髓干细胞髓腔内定植,可以促进牙髓牙本质复合体修复再生,具有潜在的临床应用价值。

关键词: 牙髓干细胞, 牙髓牙本质复合体, 再生, 修复

Abstract: Objective: To observe the potential of homogeneous dental pulp stem cells (DPSCs) to repair and regenerate pulp dentin complex when rat crown pulp is removal. Methods: Eighteen SD rats were randomly divided into experimental group and control group. The experimental group was transplanted allogeneic rats’ DPSCs into the first molar’s pulp cavity whose crown pulp was destructed. The control group was not treated after crown pulp was destructed. After2, 4, 6weeks, 18 mice were sacrificed. Their upper jaw were investigated by general observation, X-ray, HE staining, and the thickness of dentin. Results: After 4 weeks, the hyperemia of experimental group reduced. After 6 weeks, X-ray could observe the dentin of experimental group was becoming thick and root apical foramen closed. After 6 weeks, newborn dentin in experimental group obviously increased in light microscope and odontoblasts arranged palisade. The sample in experimental group showed that after 2 weeks, dentin was becoming thick, and 4 weeks was more thick than it, continued to 6 weeks. The experimental group was better than the control group. Conclusion: It had potential clinical application value that the pulp cavity as the place of dental pulp stem cells implanting could promote pulp-dentin complex’s regeneration and repairing.

Key words: Dental pulp stem cell, Pulp-dentin complex, Regeneration, Repairing

中图分类号:

R780.1

铁晓敏,刘佳,王丽萍,王璇,李伯琦,刘奕杉. 牙髓干细胞修复再生同质异体大鼠牙髓牙本质复合体的研究[J]. 口腔医学研究, 2015, 31(7): 645-648.

TIE Xiao-min, LIU Jia, WANG Li-ping, et al. Study on Dental Pulp Stem Cells(DPSCs) Regenerating Homogeneous Rats’ Pulp Dentin Complex[J]. Journal of Oral Science Research, 2015, 31(7): 645-648.

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