口腔医学研究

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (7): 692-695.DOI: 10.13701/j.cnki.kqyxyj.2016.07.007

• 基础研究论著 • 上一篇    下一篇

14,15-环氧二十碳三烯酸促进颅骨缺损骨再生

党海霞, 霍欣茹, 张文园, 张琳, 王福*   

  1. 大连医科大学口腔医学院口腔教研室 辽宁 大连 116044
  • 收稿日期:2016-01-25 出版日期:2016-07-26 发布日期:2016-07-25
  • 通讯作者: 王福,E-mail: dywangfu@gmail.com
  • 作者简介:党海霞(1987~ ),女,山西省人,硕士,主要从事颌面部发育和功能重建的研究工作。
  • 基金资助:
    大连市科学技术计划项目(编号:2015E12SF160)

14, 15- Epoxyeicosatrienoic Acid Promotes Bone Regeneration of Mouse Calvarial Defect.

DANG Hai-xia, HUO Xin-ru, ZHANG Wen-yuan, ZHANG Lin, WANG Fu*.   

  1. Department of Oral and Dental Research, School of Stomatology, Dalian Medical University, Dalian 116044, China
  • Received:2016-01-25 Online:2016-07-26 Published:2016-07-25

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摘要： 目的: 建立C57BL/6小鼠颅骨缺损模型,研究14,15-环氧二十碳三烯酸(14,15-epoxyeicosatrienoic acid,14,15-EET)能否促进骨再生,为骨组织再生研究提供新思路。方法: C57BL/6小鼠颅中缝两侧建立直径5 mm的全层颅骨圆形缺损,小鼠右侧(实验侧)缺损处植入14,15-EET预处理的骨髓间充质干细胞(BMSCs),左侧(对照侧)植入未用14,15-EET处理的BMSCs。术后第3天于实验侧缺损处局部皮下注射含14,15-EET(10 μmol/L)磷酸缓冲盐溶液(PBS),对照侧缺损处皮下注射等量PBS,隔日注射,持续4周。术后8周将小鼠处死,CBCT放射诊断观测缺损面积大小,HE染色和Masson三色染色分析新骨形成相关组织结构。结果: 术后8周小鼠实验侧缺损面积比对照侧明显减小,差异有统计学意义(P<0.05);实验侧缺损区新生血管数、新生骨量均较对照侧明显增多,差异有统计学意义(P<0.05);此外,实验侧颅骨缺损区可明显观察到骨质及胶原纤维,而对照侧不明显。结论: 通过局部提高14,15-EET水平可促进新生骨形成,推测可能与14,15-EET改善局部微循环相关。

关键词: 14, 15-环氧二十碳三烯酸, 骨缺损, 骨再生

Abstract: Objective: To evaluate the effect of 14, 15 epoxyeicosatrienoic acid (14, 15-EET) on the healing of calvarial defect in C57BL / 6 mice. Methods: A 5-mm diameter calvarial critical-sized defect was created on each side of the calvarial bone of the C57BL / 6 mice. The calvarial defects were allocated into experimental group (right sides) and control group (left sides). BMSCs pretreated with 14, 15-EET (1 μmol/L, experimental group, n =10) or untreated with 14, 15-EET (control group) were implanted into bone defects. 14, 15-EET (10 μmol/L, experimental group) or PBS (control group) was administered by subcutaneous injection in calvarial defect area every other day from third post-operative day for four weeks. Mice were sacrificed at 8th post-operative week. Bone regeneration in the calvarial defect area was assessed with CBCT. HE and Masson's trichrome stain were performed to assess the newly formed blood vessels and connective bone tissues. Results: Compared with the control sides, the area of bone defect in the experimental sides treated with 14, 15-EET was obviously decreased at 8th post-operative week (P<0.05). 14, 15-EET treatment increased blood vessels and bone formation of the experimental sides when compared to the control sides at 8th post-operative week (P<0.05). In addition, there were more osteoid and collagen fibers in the defect area treated with 14, 15- EET. Conclusion: 14, 15-EET may promote bone regeneration through improvement of local microcirculation.

Key words: 14,15-epoxyeicosatrienoic acid , Bone defect, Bone regeneration

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