口腔医学研究

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (10): 1033-1037.DOI: 10.13701/j.cnki.kqyxyj.2016.10.008

• 基础研究论著 • 上一篇    下一篇

牙髓炎不同阶段P2X受体家族在大鼠脑干中的表达变化研究

陈霞, 袁通穗, 叶芳*, 孙瑜, 杨健, 郭俊, 金幼虹   

  1. 南昌大学附属口腔医院口腔内科教研室 江西 南昌 330006
  • 收稿日期:2016-06-20 出版日期:2016-10-26 发布日期:2016-10-26
  • 通讯作者: 叶芳,E-mail:fangye999@qq.com
  • 作者简介:陈霞(1988~ ),女,湖南怀化人,住院医师,硕士,主要从事牙体牙髓及牙周病学的相关研究工作。
  • 基金资助:
    江西省自然科学基金重点项目(编号:20142BAB205035)

Expression of P2X Receptors in the Brainstem Induced by Dental Pulp Inflammation.

CHEN Xia, YUAN Tong-sui, YE Fang*, SUN Yu, YANG Jian, GUO Jun, JIN You-hong.   

  1. Department of Oral Medicine Teaching and Research Section, Affiliated Stomatological Hospital, Nanchang University, Nanchang 330006, China.
  • Received:2016-06-20 Online:2016-10-26 Published:2016-10-26

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1

摘要： 目的:研究牙髓炎不同阶段P2X受体家族在大鼠脑干中的表达变化。方法::本实验在大鼠左上颌第一磨牙颌面开髓,并暴露于口腔菌群环境,诱导牙髓炎症。取牙髓炎症第1天、第3天、第7天和第28天时,大鼠双侧脑干的三叉神经脊束核尾端亚核(Vc)和丘脑的腹后中核(VPM),采用免疫组化和免疫荧光方法观察P2X受体家族(P2X1-7受体)的表达模式。结果:实验观察到牙髓炎症第1、3、7天显示急性牙髓炎症的组织学表现,其中第3天最为典型,而第28天显示牙髓坏死和慢性根尖周炎的组织学表现;P2X1-6受体在Vc和VPM中均表达在神经元的胞浆中,而P2X7表达在Vc和VPM中在小胶质细胞中;P2X2,P2X4和P2X7受体在急性牙髓炎症期在同侧Vc中的表达增加;P2X1-5和P2X7的表达都在双侧VPM中均上升,开髓3 d时,对侧VPM中P2X1-5和P2X7的平均表达面积显著高于同侧。结论:除P2X6受体持续高表达外,P2X1-5和P2X7受体均可受到牙髓炎症的诱导,在Vc和VPM中表达增高。

关键词: P2X受体, 牙髓炎, 三叉神经节, 三叉神经脊束核尾端亚核

Abstract: Objective: To study the expression of P2X receptors in the trigeminal spinal subnucleus caudalis (Vc) and the ventral posteromedial nucleus (VPM) induced by dental pulp inflammation.Methods: Cavities were prepared on the left first upper molars via a 1/2 # round ball drill, and the cavities with exposed dental pulp were left open to the oral microflora for 1, 3, 7 and 28 days. The Vc and VPM were harvested at specific time intervals and underwent further immunofluorescent and immunohistochemical procedures to assess the expression patterns of P2X1-7.Results: Acute inflammation was induced by dental pulp exposure from 1d to 7d, and chronic inflammation around the apical area of the root was observed after 28d. The P2X1-6 receptors were expressed in neurons, whereas P2X7 was expressed in microglia. Varying increases in the expression of the P2X1-7 receptors were characterized during acute and chronic pulp inflammation. The expressions of P2X2, P2X4 and P2X7 in the ipsilateral Vc, and P2X1-5 and P2X7 in the bilateral VPM, especially on the contralateral side were increased during acute inflammation process.Conclusion: The present findings suggest that all of the P2X receptors except P2X6 are involved in different ways in the Vc and VPM after dental pulp injury.

Key words: P2X, Dental pulp inflammation, Trigeminal ganglion, Trigeminal spinal subnucleus caudalis

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