唾液乳酸杆菌w22a和种植体周围炎致病菌体外作用的初步研究

doi:10.13701/j.cnki.kqyxyj.2016.10.010

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (10): 1042-1046.DOI: 10.13701/j.cnki.kqyxyj.2016.10.010

唾液乳酸杆菌w22a和种植体周围炎致病菌体外作用的初步研究

薛龙^1,4, 王立鹤^1,4, 聂红兵^2,3*, 周海静^2,3, 郭曼丽^2,3, 康永杰^1,4, 杨兰^1*

1. 兰州大学第二医院口腔颌面外科甘肃兰州 730030;
2. 西北民族大学甘肃省口腔疾病研究重点实验室培育基地;
3. 西北民族大学口腔医学国家民委重点实验室;
4. 兰州大学口腔医学院

收稿日期:2016-03-28 出版日期:2016-10-26 发布日期:2016-10-26
通讯作者: 杨兰,E-mail:ylan2005@163.com;聂红兵,E-mail:278773109@qq.com
作者简介:薛龙(1988~),男,甘肃人,住院医师,硕士在读,主要从事口腔颌面外科及基础研究。
基金资助:
甘肃省自然科学基金(编号:1506RJZA276);国家民委科研基金(编号:14XBZ022)

Antibacterial Effects of Lactobacillus Salivarius W22a on the Periodontal Pathogens.

XUE Long^1,4, WANG Li-he^1,4, NIE Hong-bing^2,3*, ZHOU Hai-jing^2,3, GUO Man-li^2,3, KANG Yong-jie^1,4, YANG Lan^1*.

1. Department of Oral and Maxillofacial Surgery, The Second Hospital of Lanzhou University, Lanzhou 730000, China;
2. Key Laboratory of Oral Diseases of Gansu Provincial, Northwest University for Nationalities, Lanzhou 730000, China;
3.Key Lab of Stomatology of State Ethnic Affairs Commission, Northwest University for Nationalities, Lanzhou 730030, China;
4.School of Stomatology, Lanzhou University, Lanzhou 730000, China

Received:2016-03-28 Online:2016-10-26 Published:2016-10-26

摘要/Abstract

摘要： 目的:研究唾液乳酸杆菌w22a的潜在益生性和与种植体周围炎致病菌:牙龈卟啉单胞菌(Porphyromonas gingivalis,Pg)、中间普氏菌(Pevotellaintermedia,Pi)、具核梭酸杆菌(Fusobacteriumncleatum,Fn)的体外作用,为乳酸杆菌w22a作为益生菌应用于种植体周围炎的防治提供实验依据。方法::通过检测w22a代谢产物成分、w22a对抑菌物质的耐受性等,初步探讨其口腔益生特性;并通过自聚和共聚实验、抑菌实验研究唾液乳酸杆菌w22a和种植体周围炎致病菌Pg、Pi、Fn的体外作用。结果:发现唾液乳酸杆菌w22a可产生有机酸、过氧化氢等抑菌物质,并能产生胞外多糖;w22a能够耐受一定浓度的抗生素和溶菌酶;w22a在体外有自聚集和与Pg、Pi、Fn发生共聚集的能力;其代谢产物对于Pg、Pi、Fn的生长具有不同程度的抑制作用。结论:唾液乳酸杆菌w22a能够对Pg、Pi、Fn产生生长抑制作用,具有口腔益生性,有望作为益生菌用于种植体周围炎的防治。

关键词: 唾液乳杆菌, 益生菌, 种植体周围炎

Abstract: Objective: To evaluate whether the oral administration of Lactobacillus salivarius w22a could alleviate the peri-implantitis pathogens in subgingival plaque, such as Porphyromonas gingivalis (Pg), Pevotella intermedia (Pi), and Fusobacteriurn nuclearum (Fn) in vitro.Methods: The antagonistic growth effects of Lactobacillus salivarius as well as their probiotic on periodontal pathogens, including Pg, Pi and Fn, were tested using drug resistance experiment, agglutination experiments, and antibacterial experiments.Results: The results revealed that Lactobacillus salivarius w22a could produce antibiotics such as organic acid, hydrogen peroxide and EPS. Strain w22a could resist lysozyme and antibiotics at certain concentration. In addition, auto-agglutination and agglutination with other pathogens could be observed. The fermentation of w22a inhibited the growth of pathogens to varied degrees.Conclusion: Lactobacillus salivarius w22a was a potential probiotic strain to inhibit the growth of Pg, Pi and Fn in vitro.

Key words: Lactobacillus salivarius, Probiotic, Peri-implantitis

中图分类号:

R781.3

薛龙, 王立鹤, 聂红兵, 周海静, 郭曼丽, 康永杰, 杨兰. 唾液乳酸杆菌w22a和种植体周围炎致病菌体外作用的初步研究[J]. 口腔医学研究, 2016, 32(10): 1042-1046.

XUE Long, WANG Li-he, NIE Hong-bing, ZHOU Hai-jing, GUO Man-li, KANG Yong-jie, YANG Lan.. Antibacterial Effects of Lactobacillus Salivarius W22a on the Periodontal Pathogens.[J]. Journal of Oral Science Research, 2016, 32(10): 1042-1046.

参考文献

[1] Simonis P, Dufour T, Tenenbaum H. Long-term implant survival and success: a 10-16-year follow-up of non-submerged dental implants[J].Clin Oral Implants Res,2010, 21(7)∶772-777
[2] Dhir S.Biofilm and dental implant: The microbial link.[J].J Indian Soc Periodontol,2013,17(1)∶5-11
[3] Seneviratne CJ, Zhang CF, Samaranayake LP. Dental plaque biofilm in oral health and disease[J]. Chin J Dent Res, 2011, 14(2)∶87-94
[4] Quirynen M,Vogels R,Peeters W eta1. Dynamics of initial subgingival colonization of'pristine peri-implant pockets[J]. ClinOral Implants Res,2006,17(1)∶25-37
[5] Mombelli A,Schmid B, Rutar A, et al. Local antibiotic therapy guided by microbiological diagnosis[J].Clin Periodontol,2002,29(8)∶743- 749
[6] Maruyama N, Maruyama F, Takeuchi Y, et al. Intraindividual variation in core microbiota in peri-implantitis and periodontitis[J]. Scientific Reports, 2014, 4∶6602-6602
[7] Dhir S.Biofilm and dental implant: The microbial link.[J].Indian Soc Periodontol, 2013, 17(1):5-11
[8] Assche N,Van Essche M,Pauwels M,et aL. Do periodontopathogens disappear after full-mouth tooth extraction[J]. J Clin Periodontol,2009,36(12)∶1043-1047
[9] Chrcanovic BR. A History of Periodontitis Suggests a Higher Risk for Implant Loss [J]. Evid Based Dent Pract, 2015, 15(4)∶185-186
[10] Valderrama P, Blansett JA, Gonzalez MG, et al. Detoxification of Implant Surfaces Affected by Peri-Implant Disease: An Overview of Non-surgical Methods [J]. Open Dent J, 2014, 16(8)∶77-84
[11] 李璐,陆峻君.益生菌对口腔微生物的影响[J].中国微生态学杂志,2010,01∶92-93+97
[12] Garima Jindala, Ramesh Kumar Pandeyb, Rajeev Kumar Singhc, et al. Can early exposure to probiotics in children prevent dental caries? A current perspective [J]. Journal of Oral Biology and Craniofacial Research.Volume 2, Issue 2, May-August, 2012, Pages 110-115
[13] 杨颖,陈卫,田丰伟,等.产抑菌物质乳杆菌的筛选及性质的研究[J].工业微生物,2006,36(3)∶13-17
[14] 张任秀.乳杆菌DM9811脂肪酸组分抑制白色念珠菌致老年义齿性口炎的作用[J].中国老年学杂志,2011,24∶4787-4788
[15] Pangsomboon K, Kaewnopparat S, Pitakpornpreecha T, et al. Antibacterial activity of a bacteriocin from Lactobacillus paracasei HL32 against Porphyromonas gingivalis [J]. Arch Oral Biol, 2006, 51(9):784-93
[16] Nyako EA, Watson CJ, Preston AJ. Determination of the pH of peri - implant crevicular fluid in successful and failing dental implant sites:A pilot study [J]. Arch Oral Biol, 2005, 50(12)∶1055-1059
[17] 杨颖.口腔中益生乳杆菌的研究[D].江南大学,2006.DOI:10.7666/d.y1195887
[18] 杨娟,堵国成,陈坚,等.口腔乳酸杆菌的分离及其益生特性[J].微生物学报,2013,04∶403-408
[19] Kang MS,Na HS,Oh JS. Coaggregation ability of Weissella cibaria isolates with Fusobacterium nucleatum and their adhesiveness to epithelial Cells [J]. FEMS Microbiol Lett,2005,253(2)∶323-329

唾液乳酸杆菌w22a和种植体周围炎致病菌体外作用的初步研究

Antibacterial Effects of Lactobacillus Salivarius W22a on the Periodontal Pathogens.

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 14

编辑推荐

Metrics

[1]	贾永娜, 缑小蕊, 姜丹丹, 唐小雪, 周政. 3种参数Er:YAG激光治疗早期种植体周围炎的临床疗效及细菌学分析[J]. 口腔医学研究, 2020, 36(5): 486-489.
[2]	吴鹏, 高承志. 2型糖尿病患者慢性牙周炎和种植体周围炎的炎症基因表达[J]. 口腔医学研究, 2019, 35(9): 854-857.
[3]	吴佩佩,姑则丽努尔·阿布都克力木,朱亚玲,杨政尧,尼加提·吐尔逊. 维药买朱尼对犬种植体周围炎骨改建的实验研究[J]. 口腔医学研究, 2018, 34(9): 974-978.
[4]	李娜, 林崇翔, 丁熙, 邵侠, 赵瑜, 屠呈威. 大鼠微螺钉种植体周围炎外周血中Foxp3⁺调节性T细胞的水平变化[J]. 口腔医学研究, 2018, 34(7): 726-729.
[5]	杨海丽, 邹海啸, 宋莉. 光动力治疗种植体周围炎的研究进展[J]. 口腔医学研究, 2018, 34(5): 467-469.
[6]	程志芬, 崔连保, 玄延花. IL-6和Shh在种植体周围炎龈沟液中的表达及意义[J]. 口腔医学研究, 2018, 34(1): 87-89.
[7]	李维婷, 朴牧子, 李慧, 张毅, 周卉, 胡洪成, 唐志辉, 李蓬. 种植体周围疾病发病率及危险因素的研究[J]. 口腔医学研究, 2017, 33(7): 758-761.
[8]	古丽再努·依不拉音, 吐逊阿依·阿迪力, 任家卉, 杨超, 尼加提·吐尔逊. 维药买朱尼对种植体周围炎龈沟液中白细胞介素-1β和牙周指数的影响[J]. 口腔医学研究, 2017, 33(7): 762-765.
[9]	吴立立, 史秋涛, 谷志远. 白细胞介素-34与牙周炎、种植体周围炎的关系[J]. 口腔医学研究, 2017, 33(11): 1234-1236.
[10]	马国武,郑胜. rhTNFR:Fc对兔正畸微种植体周围炎骨结合影响的研究[J]. 口腔医学研究, 2016, 32(9): 916-919.
[11]	汪珍珍,余占海,吕玮,周建业,胡晓潘,焦康礼,李志强. 微生态制剂及抗菌肽在龋病防治中的研究进展[J]. 口腔医学研究, 2016, 32(9): 994-996.
[12]	孙福财,余方友,李贤. 盐酸米诺环素软膏对微螺钉支抗种植体周围炎龈下牙龈卟啉单胞菌影响[J]. 口腔医学研究, 2016, 32(4): 395-398.
[13]	赵琛,华红,闫志敏. 益生菌预防和治疗口腔疾病的研究进展[J]. 口腔医学研究, 2016, 32(4): 418-420.
[14]	吴亚婷,聂红兵,王子剑,王佳佳,周建业,郭曼丽. FTA-DNA直接提取法在种植体周围炎/牙周炎检测的效果评价[J]. 口腔医学研究, 2015, 31(11): 1089-1092.