口腔医学研究

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (2): 132-134.DOI: 10.13701/j.cnki.kqyxyj.2017.02.004

• 基础研究论著 • 上一篇    下一篇

牙龈卟啉单胞菌唾液酸酶基因缺失影响其感染上皮细胞后炎症相关microRNA的表达

李琛1, 杨雪1, 潘亚萍1*, 徐晓宇1, 佟彤1, 郭艳2   

  1. 1.中国医科大学附属口腔医院牙周病科,辽宁省口腔医学研究所牙周病学研究室,辽宁省口腔疾病转化医学研究中心 辽宁 沈阳 110002;
    2. 中国医科大学附属口腔医院中心实验室,辽宁省口腔疾病重点实验室 辽宁 沈阳 110002
  • 收稿日期:2016-09-01 出版日期:2017-02-20 发布日期:2017-02-22
  • 通讯作者: 潘亚萍,E-mail:yppan@mail.cmu.edu.cn
  • 作者简介:李琛(1980~ ),女,沈阳人,副主任医师,博士,主要从事牙周病病因学研究。
  • 基金资助:
    国家自然科学基金资助项目(编号:81200785)辽宁省自然科学基金(编号:2015010496-301)

Effects of Abrogation of Neuraminidase in Porphyromonas Gingivalis on Inflammation-related MicroRNA Expressions after Infected with Gingival Epithelial Cells.

LI Chen1, YANG Xue1, PAN Ya-ping1*, XU Xiao-yu1, TONG Tong1, GUO Yan2   

  1. 1. Department of Periodontics,School of Stomatology,Liaoning Institution of Dental Research,China Medical University,Shenyang 110002,China;
    2. Center Lab, School of Stomatology,China Medical University,Shenyang 110002,China.
  • Received:2016-09-01 Online:2017-02-20 Published:2017-02-22

摘要： 目的:检测牙龈卟啉单胞菌(Porphyromonas gingivalis,P. gingivalis)唾液酸酶基因(PG0352)缺失是否影响其感染上皮细胞后对上皮细胞炎症相关小RNA的调控。方法:厌氧培养P. gingivalis W83及其唾液酸酶基因突变株(△PG0352),以MOI为100∶1的比例分别与永生化牙龈上皮细胞epi4进行共培养,PCR array的方法检测88个与炎症相关的小RNA的表达。结果:△PG0352组epi4的hsa-miR-9、hsa-miR-23a、hsa-miR-23b和hsa-miR-410的表达分别是P. gingivalis W83组的4.3387、3.3978、4.7930和4.3137倍。结论:与P. gingivalis W83野生株相比,唾液酸酶基因缺陷株对牙龈上皮细胞炎症相关小RNA的表达有不同的调控作用。

关键词: 牙龈卟啉单胞菌W83, PG0352, 牙龈上皮细胞, 小RNA

Abstract: Objective: To investigate effects of abrogation of neuraminidase in porphyromonas gingivalis on inflammation-related microRNA expressions after infected with gingival epithelial cells. Methods: P. gingivalis W83 and PG0352 mutant strain (△PG0352) were cultured anaerobically, and co-cultured with gingival epithelial cells at the MOI of 100:1. Inflammation-related microRNAs were detected by real-time PCR. Results: The expressions of hsa-miR-9, hsa-miR-23a, hsa-miR-23b, and hsa-miR-410 were significantly higher in △PG0352 group than those in P. gingivalis W83 group. The fold changes were 4.3387, 3.3978, 4.7930 and 4.3137, respectively. Conclusion: Neuraminidase deficient strain showed different effects on inflammation-related microRNA expression in gingival epithelial cells compared with wild-type strain.

Key words: Porphyromonas gingivalis, W83 , PG0352 , Gingival epithelial cell , MicroRNA

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