基质胶缓释神经轴突导向分子SEMA3A对去势大鼠钛种植体稳定性的影响

doi:10.13701/j.cnki.kqyxyj.2017.02.003

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (2): 127-131.DOI: 10.13701/j.cnki.kqyxyj.2017.02.003

基质胶缓释神经轴突导向分子SEMA3A对去势大鼠钛种植体稳定性的影响

王鹏, 何东明, 刘彪, 胡静, 李继华, 李运峰^*

四川大学华西口腔医学院,口腔疾病研究国家重点实验室四川成都 610041

收稿日期:2016-08-07 出版日期:2017-02-20 发布日期:2017-02-22
通讯作者: 李运峰,E-mail:doctorlyf@163.com
作者简介:王鹏(1989~ ),男,硕士在读,研究方向:骨质疏松,牵张成骨。
基金资助:
国家自然科学基金(编号:81300858)教育部博士点基金(编号:20130181120090)

SEMA3A Suspended in Matrigel Improves Titanium Implant Fixation in Ovariectomized Rats.

WANG Peng, HE Dong-ming, LIU Biao, HU Jing, LI Ji-hua, LI Yun-feng^*.

State Key Laboratory of Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu 610041, China

Received:2016-08-07 Online:2017-02-20 Published:2017-02-22

摘要/Abstract

摘要： 目的:评估基质胶缓释SEMA3A对去势大鼠种植体稳定性的影响。方法:对60只SD大鼠行双侧卵巢切除术。12周后,根据植入种植体的不同将动物随机分为3组:1)对照组,种植体+蒸馏水;2)基质胶组, 种植体+基质胶;3)种植体+基质胶+SEMA3A,将种植体植入所有动物胫骨近端的干骺端。在利用酶联免疫吸附试验检测种植体表面基质胶缓释SEMA3A的生物学过程进行检测。种植体植入后的第4周和第8周将胫骨与植入体一起收集,行显微CT、组织学和生物力学检测。结果:SEMA3A的体外释放检测发现,连续10 d可以检测到基质胶中释放的SEMA3A,其中前3 d呈爆发性释放过程; 种植体植入后第4和第8周,与单独使用基质胶组相比,SEMA3A+基质胶组骨体积百分比分别增加了88.7%和83.3%,骨小梁粗度分别增加了51.4%和49.9%,骨小梁数量分别增加了54.2％和62.1％;在组织学定量评价中,种植体周围相对骨面积分别增加了89.8%和149.2%,骨与种植体结合率分别增加了148.9%和24.8%;生物力学检测发现,种植体的最大推出力增加了149.3%和209.2%,结合刚度增加171.2%和210.6%,结合韧性增加了151.8%和268.2%。结论:用SEMA3A加基质胶对种植体进行表面改性可以有效改善去势大鼠种植体的骨整合与稳定性。

关键词: 骨质疏松症, 种植体, 骨整合, SEMA3A

Abstract: Objective: To evaluate the effect of SEMA3A released from matrigel on implant fixation in ovariectomized (OVX) rats. Methods: Sixty female rats were subjected to bilateral ovariectomy. Twelve weeks later, rats were randomly divided into three groups: 1) Control group: implants with distilled water; 2) Matrigel group: implants with matrigel coating; 3) Matrigel+SEMA3A group. Implants were inserted bilaterally in metaphysis of proximal tibiae in all animals. In vitro release of SEMA3A was tested using enzyme linked immunosorbent assay. Four and eight weeks after implantation, tibiae with implants were harvested for micro-CT, histology, and biomechanical push-out test. Results: In vitro release of SEMA3A was detectable during the first ten days, and a burst release was observed during the first three days. No significant difference was observed between Control and Matrigel group. Compared to matrigel alone, SEMA3A suspended in matrigel increased percent bone volume by 88.7% and 83.3%, trabecular thickness by 51.4% and 49.9%, trabecular number by 54.2% and 62.1% in micro-CT analysis at 4 and 8 weeks, respectively. SEMA3A suspended in matrigel also increased bone area density by 89.8% and 149.2%, bone-to-implant contact ratio by 148.9% and 24.8% in histological evaluation, and the maximal push-out force by 149.3% and 209.2%, stiffness by 171.2% and 210.6%, toughness 151.8% and 268.2%, at 4 and 8 weeks, respectively. Conclusion: Surface modification with SEMA3A suspended in matrigel improved implant osseointegration and fixation in the proximal tibiae of OVX rats.

Key words: Osteoporosis , Implant , Osseointegration, Semaphorin, 3A

中图分类号:

R782.4
R683

王鹏, 何东明, 刘彪, 胡静, 李继华, 李运峰. 基质胶缓释神经轴突导向分子SEMA3A对去势大鼠钛种植体稳定性的影响[J]. 口腔医学研究, 2017, 33(2): 127-131.

WANG Peng, HE Dong-ming, LIU Biao, HU Jing, LI Ji-hua, LI Yun-feng.. SEMA3A Suspended in Matrigel Improves Titanium Implant Fixation in Ovariectomized Rats.[J]. Journal of Oral Science Research, 2017, 33(2): 127-131.

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基质胶缓释神经轴突导向分子SEMA3A对去势大鼠钛种植体稳定性的影响

SEMA3A Suspended in Matrigel Improves Titanium Implant Fixation in Ovariectomized Rats.

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