增龄性因素对正畸牙齿移动影响的实验研究

doi:10.13701/j.cnki.kqyxyj.2017.02.002

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (2): 123-126.DOI: 10.13701/j.cnki.kqyxyj.2017.02.002

增龄性因素对正畸牙齿移动影响的实验研究

周新文¹, 曲圣爱², 徐辉^2*

1. 首都医科大学附属北京世纪坛医院口腔科北京 100038;
2. 首都医科大学附属北京口腔医院正畸科北京 100050

收稿日期:2016-07-10 出版日期:2017-02-20 发布日期:2017-02-22
通讯作者: 徐辉,E-mail:huixudentist@163.com
作者简介:周新文(1971~ ),男,甘肃省人,副主任医师,博士,主要从事口腔临床及基础的研究工作。
基金资助:
国家自然科学基金(编号:61102015)北京市卫生系统高层次卫生技术人才培养计划(编号:2014-3-078)

Experimental Study on Influence of Aging Factors on Orthodontic Tooth Movement.

ZHOU Xin-wen¹, QU Sheng-ai², XU Hui^2*

1. Department of Orthodontics, School of Stomatology, Capital Medical University, Beijing 100050, China;
2. Department of Stomatology, Beijing Sijitan Hospital, Capital Medical University, Beijing 100038, China

Received:2016-07-10 Online:2017-02-20 Published:2017-02-22

摘要/Abstract

摘要： 目的:探索增龄性因素对正畸牙移动过程中牙周组织内HIF-1α及VEGF的影响。方法:建立不同年龄组的正畸牙齿移动动物模型,加力1 d、3 d、7 d、14 d及28 d,用免疫组化法检测牙周组织中HIF-1α及VEGF的表达情况。结果:青少年组大鼠实验侧压力区HIF-1α表达在加力1 d、3 d时明显高于成年组大鼠;VEGF表达量在加力1 d、3 d、7 d、14 d时明显高于成年组大鼠。结论:增龄性因素可使牙周组织对矫治力的反应速率降低,缺氧调控速率减慢,最终使牙槽骨改建减慢,牙移动速率降低。VEGF参与了正畸牙移动中组织改建过程,但成年组大鼠正畸牙齿移动过程中VEGF表达量增加缓慢,使牙槽骨改建速度降低,造成牙齿移动速度减慢。

关键词: 牙齿移动, 牙周组织, 增龄, HIF-1α, VEGF

Abstract: Objective: To explore the effects of aging factors on the expression of HIF-1α and VEGF in the periodontal tissues during orthodontic tooth movement in rats. Methods: Orthodontic tooth movement animal models with different ages were established. The expressions of HIF-1α and VEGF in the periodontal tissues were detected by immunohistochemistry method at day 1, 3, 7, 14 and 28 after orthodontic force application. Results: The expression of HIF-1α in the young group was significantly higher than that in the adult group at day 1 and 3. The expression of VEGF was higher than that in the adult group at day 1, 3, 7 and 14. Conclusion: Aging factors can decrease the reaction of the periodontal tissue, and slow down the rate of hypoxia regulation. Finally, the alveolar bone remodeling and the speed of tooth movement would decrease. VEGF plays an important role in the periodontal tissue remodeling. But the expression of VEGF increases slowly in the adult group. The speed of alveolar bone reconstruction reduced, as well as the speed of tooth movement.

Key words: Tooth movement , Periodontal tissue, Aging , HIF-1α , VEGF

中图分类号:

R783.5

周新文, 曲圣爱, 徐辉. 增龄性因素对正畸牙齿移动影响的实验研究[J]. 口腔医学研究, 2017, 33(2): 123-126.

ZHOU Xin-wen, QU Sheng-ai, XU Hui. Experimental Study on Influence of Aging Factors on Orthodontic Tooth Movement.[J]. Journal of Oral Science Research, 2017, 33(2): 123-126.

参考文献

[1] Morse DR. Age related changes of the dental pulp complex and their relationship to systemic; aging. Oral Surg oral Med Oral Pathol,1991,72(6)∶721-726
[2] 唐新宝, 刘静.正畸牙移动中牙周组织内VEGF动态变化的研究 [J]. 实用医学杂志,2005,21(14)∶1502-1504
[3] Gordillo GM,Sen CK.Revisiting the essential role of oxygen in wound healing [J]. Am J Surg, 2003,186(3)∶259-263
[4] Street JT, Lenehan B, Deschrijjveg K,et al. Hypoxia regulates the paracrine coupling of angiogenesis and bone formation [J].Eur J Orthop Surg Traumatol, 2005, 15(3)∶214-225
[5] 陈斌,李琥,穆超,等.大鼠正畸牙齿移动过程中 HIF-1α的表达[J].口腔生物医学,2011,02(6)∶78-81
[6] Gerber HP, Ferrara N. The role of VEGF in normal and neoplastic hematopoiesis [J]. Mol Med,2003,81(1)∶20-31
[7] Ahluwalia A, Jones MK, Deng X, et al. An imbalance between VEGF and endostatin underlies impaired angiogenesis in gastric mucosa of aging rats[J]. Am J Physiol Gastrointest Liver Physiol, 2013, 305∶G325-G332
[8] 王莹,王兰珠,韩博,等.正畸力作用下压力侧 Wnt10b、Rankl在牙周组织中的表达[J].口腔医学研究,2016,32(3)∶257-260
[9] Andreollo NA1, Santos EF, Araújo MR, Lopes LR. Rat's age versus human's age: what is the relationship? Arq Bras Cir Dig, 2012 ,25(1)∶49-51
[10] Ren Y, Maltha JC, Van 't Hof MA,et al. Age effect on orthodontic tooth movement in rats[J]. J Dent Res, 2003, 82(1)∶38-42
[11] Ren Y, Maltha JC, Stokroos L, et al. Age-related changes of periodontal ligament surface areas during force application[J]. Angle Orthod, 2008, 78(6)∶1000-1005
[12] King GJ, Latta L, Rutenberg J, et al. Alveolar bone turnover in male rats: site-and age-specific changes[J]. Anat Rec, 1995, 242(3)∶321-328
[13] Amemiya H,Matsuzaka K,Kokubu E,et al.Cellular responses of rat periodontal ligament cells under hypoxia and re-oxygenation conditions in vitro[J]. J Periodontal Res, 2008, 43(3)∶322-327
[14] Kohno A, Kaku M, Tsutsui K, et al. Expression of vascuIar endothelial growth factor and the effects on bone remodeling during experimental tooth movement[J]. J Dent Res, 2003,82(3)∶117-182

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增龄性因素对正畸牙齿移动影响的实验研究

Experimental Study on Influence of Aging Factors on Orthodontic Tooth Movement.

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