口腔医学研究

口腔医学研究 ›› 2018, Vol. 34 ›› Issue (2): 139-143.DOI: 10.13701/j.cnki.kqyxyj.2018.02.008

• 口腔肿瘤学研究 • 上一篇    下一篇

普萘洛尔对人黑色素瘤A375细胞增殖迁移和血管生成能力的影响及其机制研究

邹海啸1,2, 杨海丽1, 赵怡芳3, 宋莉1*   

  1. 1. 南昌大学第二附属医院口腔科 江西 南昌 330006;
    2. 口腔基础医学省部共建国家重点实验室培育基地和口腔生物医学教育部重点实验室 湖北 武汉 430079;
    3. 武汉大学口腔医院口腔颌面头颈肿瘤外科 湖北 武汉 430079;
  • 收稿日期:2017-10-16 出版日期:2018-02-28 发布日期:2018-02-26
  • 通讯作者: 宋莉,E-mail:ndefy91009@ncu.edu.cn
  • 作者简介:邹海啸(1985~ ),男,湖北武汉人,博士,主要从事口腔颌面部肿瘤的临床和基础研究。
  • 基金资助:
    国家自然科学基金资助项目(编号:81502745)
    江西省科技厅重点项目(编号:20151BBG70074)

Effect and Mechanism of Propranolol on Proliferation, Migration, and Angiogenesis of Human Melanoma A375 cells

ZOU Hai-xiao1,2, YANG Hai-li1, ZHAO Yi-fang3, SONG Li1*   

  1. 1. Department of Stomatology, The Second Affiliated Hospital of Nanchang University, Nanchang 330006, China;
    2. The State Key Laboratory Breeding Base of Basic Science of Stomatology, Hubei Province & Key Laboratory of Oral Biomedicine, Wuhan 430079, China;
    3. Department of Oral and Maxillofacial Head And Neck Cancer Surgery, Stomatology Hospital of Wuhan University, Wuhan 430000, China.
  • Received:2017-10-16 Online:2018-02-28 Published:2018-02-26

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摘要： 目的:探讨普萘洛尔对人黑色素瘤A375细胞增殖、迁移和促血管生成能力的影响和机制。方法:将1、5、10、20 μmol/L普萘洛尔作用于体外培养的A375细胞,MTT法检测普萘洛尔对A375细胞增殖能力的影响;细胞划痕实验测定普萘洛尔对A375细胞的迁移能力的影响;小管形成实验评估普萘洛尔对A375细胞促血管生成能力的影响;RT-PCR和 Western bolt分别检测普萘洛尔对A375细胞VEGF、MMP-9、CXCR4 mRNA 和蛋白表达的影响。结果:与对照组相比,普萘洛尔对A375细胞的增殖能力无显著性抑制,呈浓度依赖性抑制A375细胞的迁移能力和促血管生成能力,并且可显著性下调A375细胞中VEGF、MMP-9、CXCR4 mRNA 和蛋白的表达(P＜0.01) 。结论:普萘洛尔可能通过下调VEGF、MMP-9和CXCR4的表达发挥抑制A375细胞的迁移和促血管生成能力的作用。

关键词: 普萘洛尔, 黑色素瘤, VEGF, MMP-9, CXCR4

Abstract: Objective: To investigate the effects and its mechanism of different dose of propranolol on the proliferation, migration, and angiogenesis of human melanoma A375 cells. Methods: A375 cells were treated with 1, 5, 10 and 20 μmol/L propranolol respectively in vitro. MTT method was used to detect the effects of propranolol on the proliferation ability of A375 cells. Wound healing assay was used to detect the effect of propranolol on the cell migration. Tube formation assay was used to investigate the angiogenesis ability of A375 cells on the HUVEC. RT-PCR and western bolt were used to assay the effect of propranolol on the expression of VEGF, MMP-9, and CXCR4. Results: Propranolol did not significant affect the proliferation of A375 cells. It might suppress the migration, tube formation, and pro-angiogenesis ability of A375 cells by inhibiting the expression of VEGF, MMP-9, and CXCR4 in a dose dependent manner. Conclusion: Propranolol may play a role in suppressing the migration and angiogenesis of A375 cells by inhibiting the expression of VEGF, MMP-9, and CXCR4.

Key words: Propranolol, Melanoma, VEGF, MMP-9, CXCR4

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