口腔医学研究

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (2): 179-182.DOI: 10.13701/j.cnki.kqyxyj.2017.02.015

• 临床研究论著 • 上一篇    下一篇

干细胞因子在人慢性根尖周炎组织中表达的研究

沈苏倩, 李娟, 黄世光*   

  1. 暨南大学医学院口腔医学系 广东 广州 510632
  • 收稿日期:2016-06-14 出版日期:2017-02-20 发布日期:2017-02-22
  • 通讯作者: 黄世光,E-mail:thshg@126.com
  • 作者简介:沈苏倩(1990~ ),女,江苏人,硕士在读,研究方向是干细胞因子在人根尖周炎组织中的表达。
  • 基金资助:
    广东省社会发展领域科技计划项资助项目 (编号:2013B021800043、2014A020212212)

Expression of Stem Cell Factor in the Periapical Tissues of Human Chronic Periapical Diseases.

SHEN Su-qian, LI Juan, HUANG Shi-guang*   

  1. Department of Stomatology, Medical College, Jinan University, Guangzhou 510632, China.
  • Received:2016-06-14 Online:2017-02-20 Published:2017-02-22

摘要： 目的:观察干细胞因子(stem cell factor, SCF)在人慢性根尖周炎病变组织中成纤维细胞(fibroblasts, FBs)、内皮细胞(endothelial cells, ECs)及巨噬细胞(macrophages, m )上的表达,探讨SCF阳性的FBs、ECs及m 在人慢性根尖周炎发病机制中的作用。方法:本实验共收集50例根尖周组织标本,其中根尖周肉芽肿组15例,根尖周囊肿组15例,正常对照组20例。组织标本经福尔马林液固定,石蜡包埋、制作连续切片。HE染色,光学显微镜下观察各组标本的组织学变化;经免疫荧光双染色(double immunofluorescence, DIF),在荧光显微镜下观察SCF在根尖周组织FBs、ECs及m 上表达的情况。结果:DIF结果显示,两组慢性根尖周炎组织中的CD334-SCF双阳性FBs、CD31-SCF双阳性ECs及CD14-SCF双阳性m 密度较正常对照组显著增多(P＜0.01);根尖周囊肿组与根尖周肉芽肿组的CD334-SCF双阳性FBs及CD31-SCF双阳性ECs密度无显著性差异;根尖周肉芽肿组CD14-SCF双阳性m 密度明显高于根尖周囊肿组(P＜0.01)。结论:结果提示CD334-SCF双阳性FBs、CD31-SCF双阳性ECs及CD14-SCF双阳性m 与慢性根尖周炎的发生、发展及致病机制有关。

关键词: 慢性根尖周炎, 干细胞因子, 成纤维细胞, 内皮细胞, 巨噬细胞

Abstract: Objective: To investigate the expression of stem cell factor (SCF) on fibroblasts (FBs), endothelial cells (ECs) and macrophages (m) in the periapical tissues of human chronic periapical diseases, and to analyze the role of CD334-SCF double positive FBs, CD31-SCF double positive ECs and CD14-SCF double positive m in the pathogenesis of periapical diseases. Methods: A total of 50 specimens, including healthy control (n=20), periapical cyst (n=15), and periapical granuloma (n=15) were involved. The tissue samples were fixed in formalin, embedded in paraffin, and then stained with hematoxylin and eosin for histopathology. Double immunofluorescence was employed to identity CD334-SCF double positive FBs, CD31-SCF double positive ECs and CD14-SCF double positive m . Results: Compared with healthy control, the densities of CD334-SCF double positive FBs, CD31-SCF double positive ECs and CD14-SCF double positive m were significantly higher (P <0.01). The densities of CD334-SCF double positive FBs and CD31-SCF double positive ECs in periapical cyst group had no significant difference with those in periapical granuloma group. The densities of CD14-SCF double positive m in periapical granuloma group were significantly higher than those in periapical cyst group (P＜0.01). Conclusion: The results suggest that the CD334-SCF double positive FBs, CD31-SCF double positive ECs and CD14-SCF double positive m may play important roles in the initiation, development and pathogenesis of the human chronic periapical diseases.

Key words: Chronic periapical diseases, Stem cell factor , Fibroblasts , Endothelial cells, Macrophages

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