Effect of Tobacco Extract Coenzyme Q10 on Immunologic Balance of Treg/T17 Cells in Mice with Periodontitis

doi:10.13701/j.cnki.kqyxyj.2020.06.016

Abstract

Abstract: Objective: To investigate the effect of tobacco extract coenzyme Q10 (CoQ10) on the immunologic balance of Treg/T17 cells in mice with periodontitis. Methods: 36 healthy male mice were divided into control group, model group, tinidazole group, CoQ10 low dose group, CoQ10 medium dose group, and CoQ10 high dose group, with 6 rats in each group, and were given gavage for 6 weeks. The mice model of periodontitis was established by ligation. Alveolar bone loss (ABL) value and alveolar bone resorption were measured in mice. Hematoxylin-eosin (HE) staining was used to detect periodontal histological changes in mice. Flow cytometry was used to analyze the proportions of CD4⁺ T cells, CD4⁺ Treg cells, and CD4⁺ T17 cells in peripheral blood mononuclear cells (PBMC) of mice. The levels of serum TGF-β1, IL-10, and IL-17 were detected by ELISA. Results: Compared with the control group, the ABL values of model group, tinidazole group, and various CoQ10 groups were significantly higher (P<0.05). Compared with the model group, the ABL values of tinidazole group and various CoQ10 groups decreased significantly (P<0.05). Compared with the model group, the periodontal ligament fibers in tinidazole group and various CoQ10 groups were arranged neatly, and the inflammatory cell infiltration in connective tissues was reduced. Compared with the control group, the percentages of CD4⁺ T cells, CD4⁺ T17 cells, and the level of IL-17A in peripheral blood PBMC of model group mice increased (P<0.05), and the percentage of CD4⁺ Treg cells and the levels of TGF-β and IL-10 decreased (P<0.05). Compared with model group, the percentage of CD4⁺ T cells, CD4⁺ T17 cells, and the level of IL-17A in tinidazole group and CoQ10 groups decreased (P<0.05), and the percentage of CD4⁺ Treg cells and the levels of TGF-β and IL-10 increased (P<0.05). Conclusion: Tobacco extract CoQ10 can regulate the immune balance of Treg/T17 cells in mice and alleviate periodontitis.

Key words: periodontitis mice, tobacco extract, coenzyme Q10, Treg cells, T17 cells, immunologic balance

WU Yunfei, ZHANG Li, ZHANG Xufeng, FU Qiya. Effect of Tobacco Extract Coenzyme Q10 on Immunologic Balance of Treg/T17 Cells in Mice with Periodontitis[J]. Journal of Oral Science Research, 2020, 36(6): 572-576.

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