Effects of Satellite Glia Cells Activation in Masseter Allodynia Induced by Restraint Stress in Rats.

Abstract

Abstract: Objective: To investigate the role of satellite glia cells (SGCs) activation and substance P (SP) expression in the trigeminal ganglion (TG) in the increased masseter mechanical sensitivity induced by psychological stress in rats. Methods: Forty male Sprague-Dawley rats were randomly divided into control group, 3-day, 7-day, and 14-day stress group (n=10). The rats were subjected to restraint stress 6 hours per day. The body weight gain and open field test were employed to evaluate the animals' stress condition. The electronic Von-Frey anesthesiometer were used to evaluate the masseter mechanical sensitivity. The expression of glial fibrillary acidic protein (GFAP) and SP was detected by immunofluorescent staining. Results: The rats in the stress group showed significant slower body weight increase, anxious and depressive behavior compared to control group. The masseter mechanical sensitivity of rats in the stress group was significantly elevated. The expression levels of GFAP and SP in TG were significantly up-regulated in the stress groups compared with control group. Conclusion: Chronic restraint stress could result in satellite glia cells activation and elevated SP expression in TG, indicating that the satellite glia cells activation may be involved in the increased masseter mechanical sensitivity induced by psychological stress in rats.

CLC Number:

R745.1⁺1

LIU Yang, MIAO Li, ZHAO Ya-juan, WANG Jian, ZHANG Min, ZHAO Rui-ni, CHEN Yong-jin, ZHAO Wen-feng.. Effects of Satellite Glia Cells Activation in Masseter Allodynia Induced by Restraint Stress in Rats.[J]. Journal of Oral Science Research, 2015, 31(12): 1174-1178.

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