iRoot BP Plus对小鼠炎性骨破坏作用的研究

doi:10.13701/j.cnki.kqyxyj.2016.02.002

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (2): 113-116.DOI: 10.13701/j.cnki.kqyxyj.2016.02.002

iRoot BP Plus对小鼠炎性骨破坏作用的研究

刘珊张洁朱玲新彭彬^*

武汉大学口腔医学院口腔生物医学工程教育部重点实验室湖北武汉 430079

收稿日期:2015-07-31 出版日期:2016-02-28 发布日期:2016-03-10
通讯作者: 彭彬,E-mail:phs301@vip.163.com
作者简介:刘珊(1991~),女,湖南人,博士在读,主要从事牙髓病学的临床与基础研究。
基金资助:
国家自然科学基金资助项目(编号:81371131、81570973)

Effects of iRoot BP Plus on Inflammatory Bone Destruction in Mice.

LIU Shan, ZHANG Jie, ZHU Ling-xin, PENG Bin.

School and Hospital of Stomatology, Key Lab for Oral Biomedical Engineering of Ministry of Education, Wuhan University, Wuhan 430079, China

Received:2015-07-31 Online:2016-02-28 Published:2016-03-10

摘要/Abstract

摘要： 目的:比较新型口腔生物陶瓷材料iRoot BP Plus和传统材料三氧化矿化聚合物(MTA)对LPS诱导小鼠炎症性骨破坏的影响。方法:24只BABL/C小鼠随机平分为4组,分别注射PBS、LPS(10 mg/kg)、iRoot BP Plus浸提液+LPS和MTA浸提液+LPS,1次/d。7 d后取颅盖骨,通过显微CT扫描、HE染色、酶组织化学和组织免疫荧光染色,观察骨破坏程度、破骨细胞以及组织蛋白酶K的表达。结果:iRoot BP Plus和MTA可有效减轻小鼠颅盖骨炎性骨破坏,抑制破骨细胞的形成和吸收能力,并明显降低与破骨功能相关的组织蛋白酶K的表达。结论: iRoot BP Plus对小鼠炎性骨破坏有抑制作用。

关键词: iRoot, BP, Plus, 三氧化矿化聚合物, 内毒素, 骨破坏, 破骨细胞

Abstract: Objective: To compare the effects of new oral bioceramic material iRoot BP Plus and mineral trioxide aggregate (MTA) on LPS-induced bone destruction in mice. Methods: 24 BABL/C mice were randomly equally divided into four groups injected by 10 mg/kg LPS with or without the material extracts or culture medium alone once a day. After treatment for 7 days, mice were sacrificed and calvarias were detected by micro-computed tomography scanning, histologic analysis and double immunofluorescence staining. Results: iRoot BP Plus and MTA significantly alleviated bone destruction in mice calvarias, inhibited the formation and resorption capacity of osteoclasts, and markedly downregulated the expression level of osteoclastogenic cathepsin K. Conclusion: iRoot BP Plus had inhibitory effects on inflammatory bone destruction in mice.

中图分类号:

R318.08

刘珊,张洁,朱玲新,彭彬. iRoot BP Plus对小鼠炎性骨破坏作用的研究[J]. 口腔医学研究, 2016, 32(2): 113-116.

LIU Shan, ZHANG Jie, ZHU Ling-xin, PENG Bin.. Effects of iRoot BP Plus on Inflammatory Bone Destruction in Mice.[J]. Journal of Oral Science Research, 2016, 32(2): 113-116.

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iRoot BP Plus对小鼠炎性骨破坏作用的研究

Effects of iRoot BP Plus on Inflammatory Bone Destruction in Mice.

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