口腔医学研究

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (7): 696-698.DOI: 10.13701/j.cnki.kqyxyj.2016.07.008

• 基础研究论著 • 上一篇    下一篇

天然衍生脱细胞牛心包复合BMP-2转染的BMSCs引导骨组织再生的体外实验研究

白明海1*, 张婷婷2, 吴汉江3, 赵志河4, 何正权1, 刘兴玉1   

  1. 1. 长沙市口腔医院正畸科 湖南 长沙 410005;
    2. 中南大学湘雅二医院检验科 湖南 长沙 410011;
    3. 中南大学湘雅二医院口腔科 湖南 长沙 410011;
    4. 四川大学华西口腔医院 四川 成都 600041
  • 收稿日期:2016-03-11 出版日期:2016-07-26 发布日期:2016-07-25
  • 通讯作者: 白明海,电话:13574854875
  • 作者简介:白明海(1977~ ),男,河北人,博士后,副主任医师,主要研究方向:口腔正畸低摩擦力高效矫治及相关基础研究,口腔医学生物材料。
  • 基金资助:
    长沙市科技局项目(编号:0803160-32)

Experimental Study on Acellular Bovine Pericardium Combined with BMP-2 Transfected BMSCs in Guided Bone Regeneration in Vitro.

BAI Ming-hai1*, ZHANG Ting-ting2, WU Han-jiang3, ZHAO Zhi-he4, HE Zheng-quan1, LIU Xing-yu1.   

  1. 1. Department of Orthodontics, Changsha Stomatological Hospital, Changsha 410005, China;
    2. Department of Clinical Laboratory, The Second Xiangya Hospital of Central South University, Changsha 410011, China;
    3. Department of Stomatology, The Second Xiangya Hospital of Central South University, Changsha 410011, China;
    4. West China School & Hospital of Stomatology, Sichuan University, Chengdu 600041, China
  • Received:2016-03-11 Online:2016-07-26 Published:2016-07-25

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摘要： 目的: 研究天然衍生脱细胞牛心包复合BMP-2转染BMSCs引导骨组织再生的可行性和有效性。方法: 天然衍生脱细胞牛心包体外复合BMP-2转染BMSCs,SEM评估细胞生长与增殖,MTT法检测牛心包对转染细胞的毒性,ELISA 检测BMP-2含量,结晶紫染色法测定BMSCs细胞粘附能力,ALP活性实验检测转染BMSCs成骨性能。结果: SEM发现天然衍生脱细胞牛心包复合BMP-2基因转染的BMSCs后,细胞生长并增殖良好;各组细胞的存活率没有显著性差异;天然衍生脱细胞牛心包增强了BMP-2转染BMSCs的细胞粘附能力和成骨细胞转化能力。结论: 天然衍生脱细胞牛心包复合BMP-2基因转染BMSCs后,具有良好的生物和细胞相容性,有望成为一种优良的天然GBR膜材料。

关键词: 天然衍生脱细胞牛心包, BMP-2转染的BMSCs, 引导骨组织再生, 体外实验

Abstract: Objective: To study the feasibility of naturally derived acellular bovine pericardium combined with BMP-2 gene transfected BMSCs in guided bone tissue regeneration in vitro. Methods: BMP-2 transfected BMSCs were seeded on the natural acellular bovine pericardium. The cell growth and proliferation on acellular bovine pericardium were assessed by SEM, the cytotoxicity of membrane on transfected cells was assessed by MTT test, the BMP-2 content was measured by ELISA, cell adhesion capacity was detected by crystal violet staining, and osteogenic properties was evaluated by ALP activity. Results: SEM showed that the BMP-2 gene transfected BMSCs could adhere to the acellular bovine pericardium and proliferate well. There was no significant difference in the survival rate of cells in each group. The cell adhesion capacity and the ability of BMP-2 transfected BMSCs transforming into osteoblasts were enhanced by the acellular bovine pericardium. Conclusion: Natural acellular bovine pericardium had well biological and cellular compatibility, which might be used as a good natural GBR membrane material.

Key words: Natural acellular bovine pericardium, BMP-2 gene transfected , BMSCs, GBR , In vitro study

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