IL-27在人慢性根尖周病中免疫调节活性的研究

doi:10.13701/j.cnki.kqyxyj.2016.07.016

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (7): 728-732.DOI: 10.13701/j.cnki.kqyxyj.2016.07.016

IL-27在人慢性根尖周病中免疫调节活性的研究

李娟, 沈苏倩, 梁泽梓, 林绮蕾, 黄世光^*

暨南大学医学院口腔医学系广东广州 510632

收稿日期:2015-11-17 出版日期:2016-07-26 发布日期:2016-07-25
通讯作者: 黄世光,E-mail: thshg@126.com
作者简介:李娟(1986~ ),女,广东人,硕士,医师及助理实验师,主要从事口腔内科临床治疗及实验指导。
基金资助:
广东省社会发展领域科技计划项资助项目(编号:2013B021800043;2014A020212212)

[doi] 10.13701/j.cnki.kqyxyj.2016.07.016

Immunomodulatory Activity of Interleukin-27 in Human Chronic Periapical Diseases.

LI Juan, SHEN Su-qian, LIANG Ze-zi, LIN Qi-lei, HUANG Shi-guang^*.

Faculty of Dentistry, School of Medicine, Jinan University, Guangzhou 510632, China

Received:2015-11-17 Online:2016-07-26 Published:2016-07-25

摘要/Abstract

摘要： 目的: 观察慢性根尖周病组织中IL-27在免疫细胞上的表达情况,探讨IL-27在其发病机制中的作用。方法: 60例自愿参与的受试者按根尖周病分类标准分为3组:正常对照组20例,根尖周肉芽肿组20例,根尖周囊肿组20例。HE染色,光学显微镜下观察组织学变化;免疫荧光双染色法(double immunofluorescence,DIF),荧光显微镜及共聚焦显微镜下分别观察IL-27-tryptase(肥大细胞标记蛋白)、IL-27-CD14(单核吞噬细胞标记蛋白)、IL-27-CD31(内皮细胞标记蛋白)双阳性细胞的表达。结果: 与正常对照组相比,根尖周病组中3种双阳性细胞百分比(%)明显升高(P<0.01);囊肿组的IL-27-tryptase双阳性细胞百分比显著高于肉芽肿组(P<0.01);肉芽肿组与囊肿组的IL-27-CD14、IL-27-CD31双阳性细胞百分比无显著性差异。结论: IL-27在人慢性根尖周组织肥大细胞(Mast cells, MCs)、单核吞噬细胞(Mononuclear phagocyte cells, MPs)、内皮细胞(Endothelial cells, ECs)等免疫细胞上均有不同程度的表达;IL-27-tryptase双阳性细胞可能在慢性根尖周病的致病机制,尤其在根尖周囊肿的纤维组织形成中发挥作用。

关键词: 慢性根尖周病, 白细胞介素-27, 肥大细胞, 单核吞噬细胞, 内皮细胞

Abstract: Objective: To observe the expression of IL-27 on different cells in the periapical tissues of human chronic periapical diseases. Methods: The periapical tissue specimens of 60 donors including healthy control (n=20), periapical granuloma (n=20) and radicular cyst (n=20) were stained with hematoxylin and eosin for histopathology, and double-immunofluorescence for identification of IL-27-tryptase (MCs), IL-27-CD14 (MPs) and IL-27-CD31 (ECs) double-positive cells. Results: Compared with the healthy control, the percentages (%) of double-positive cells were significantly increased in human chronic periapical diseases (P<0.01); the percentage of IL-27-tryptase double positive cells in radicular cyst was significantly higher than those in periapical granuloma (P<0.01); the percentages of IL-27-CD14 and IL-27-CD31 double-positive cells in periapical granuloma were not significant different from those in radicular cyst. Conclusion: IL-27 expressed in MCs, MPs and ECs of human chronic periapical diseases with different degree; IL-27-tryptase double-positive cells may participate in the pathogenic mechanism of chronic periapical diseases, especially in the formation of fibrous tissue in periapical cysts.

Key words: Periapical diseases , Interleukin-27 , Mast cells , Mononuclear phagocyte cells , Endothelial cells

中图分类号:

R781.4

李娟, 沈苏倩, 梁泽梓, 林绮蕾, 黄世光. IL-27在人慢性根尖周病中免疫调节活性的研究[J]. 口腔医学研究, 2016, 32(7): 728-732.

LI Juan, SHEN Su-qian, LIANG Ze-zi, LIN Qi-lei, HUANG Shi-guang.. [doi] 10.13701/j.cnki.kqyxyj.2016.07.016

Immunomodulatory Activity of Interleukin-27 in Human Chronic Periapical Diseases.[J]. Journal of Oral Science Research, 2016, 32(7): 728-732.

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IL-27在人慢性根尖周病中免疫调节活性的研究

[doi] 10.13701/j.cnki.kqyxyj.2016.07.016

Immunomodulatory Activity of Interleukin-27 in Human Chronic Periapical Diseases.

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IL-27在人慢性根尖周病中免疫调节活性的研究

[doi] 10.13701/j.cnki.kqyxyj.2016.07.016Immunomodulatory Activity of Interleukin-27 in Human Chronic Periapical Diseases.

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[doi] 10.13701/j.cnki.kqyxyj.2016.07.016

Immunomodulatory Activity of Interleukin-27 in Human Chronic Periapical Diseases.