口腔医学研究

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (9): 983-986.DOI: 10.13701/j.cnki.kqyxyj.2016.09.022

• 临床研究论著 • 上一篇    下一篇

长链非编码RNA AFAP1-AS1在口腔鳞状细胞癌中的表达及临床意义

付丛1*,张行炜2   

  1. 1. 南昌大学附属口腔医院正畸科 江西 南昌 330006;
    2. 南昌市中道口腔门诊部 江西 南昌 330006
  • 收稿日期:2016-03-07 出版日期:2016-09-26 发布日期:2016-09-26
  • 通讯作者: 付丛,E-mail:309256826@qq.com
  • 作者简介:付丛(1986~ ),女,江西人,医师,硕士,主要从事口腔鳞癌发病机制研究工作。

Expression of Long Non-coding RNA AFAP1-AS1 and Its Clinicopathologic Significance in Oral Squamous Cell Carcinoma

FU Cong1*, ZHANG Xing-wei2   

  1. 1. Department of Orthodontics, the Affiliated Stomatological Hospital of Nanchang University, Nanchang 330006, China;
    2. Dental Clinic of Zhongdao, Nanchang 330006, China.
  • Received:2016-03-07 Online:2016-09-26 Published:2016-09-26

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摘要： 目的:探讨长链非编码RNA (lncRNA) 肌动蛋白纤维相关蛋白1-反义RNA1(AFAP1-AS1)在口腔鳞癌及癌旁组织中的表达及其临床意义。方法:利用实时定量PCR(RT-qPCR)法检测75例口腔鳞癌组织及其配对癌旁正常组织中lncRNA AFAP1-AS1的表达情况,并分析其与口腔鳞癌临床病理学特征之间的联系;应用小干扰RNA(siRNA)干扰lncRNA AFAP1-AS1表达,CCK-8实验检测lncRNA AFAP1-AS1对口腔鳞癌细胞株Tca8113细胞增殖能力的影响。结果:RT-qPCR检测结果显示lncRNA AFAP1-AS1在癌组织中的相对表达水平是癌旁组织的5.16倍,差异有统计学意义(P＜0.01)。lncRNA AFAP1-AS1的表达与临床分期、分化程度及淋巴结转移显著相关(P＜0.05)。CCK-8实验结果显示,转染48 h、72 h实验组A450值较对照组显著降低(P＜0.01),表明siRNA沉默AFAP1-AS1表达后,抑制了Tca8113细胞的增殖。结论:lncRNA AFAP1-AS1在口腔鳞癌中的表达上调可能与口腔鳞癌的发生发展有关。

关键词: 口腔鳞状细胞癌, 长链非编码RNA, 肌动蛋白纤维相关蛋白1-反义RNA1, 细胞增殖

Abstract: Objective: To investigate the expression of long non-coding RNA (lncRNA) (actin filament-associated protein 1-antisense RNA1, AFAP1-AS1) and its association with clinicopathologic features in oral squamous cell carcinoma (OSCC). Methods: The expression of AFAP1-AS1 was evaluated by real-time quantitative polymerase chain reaction (RT-qPCR) in 75 tumor tissues of OSCC patients and matched adjacent tissues. The correlation between AFAP1-AS1 expression and clinicopathologic features of OSCC patients was analyzed. LncRNA AFAP1-AS1 siRNA was transfected into Tca8113 cells, and CCK-8 kit was used to detect the cell proliferation. Results: The relative expression levels of lncRNA AFAP1-AS1 in OSCC tissues were 5.16 times of that in adjacent normal tissues (P＜0.01). High AFAP1-AS1 expression was associated with cell differentiation, lymphatic metastasis and pathological stage of OSCC (P＜0.05). Furthermore, inhibition of AFAP1-AS1 expression suppressed the proliferation of human tongue carcinoma Tca8113 cells (P＜0.01). Conclusion: Up-regulation of AFAP1-AS1 in tumor tissue might act as an oncogene and promote the development of OSCC.

Key words: Oral squamous cell carcinoma, LncRNA, AFAP1-AS1, Proliferation

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