硅酸二钙对小鼠巨噬细胞系RAW264.7的促炎反应机制研究

doi:10.13701/j.cnki.kqyxyj.2017.01.007

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (1): 29-32.DOI: 10.13701/j.cnki.kqyxyj.2017.01.007

硅酸二钙对小鼠巨噬细胞系RAW264.7的促炎反应机制研究

赖世翔,陈良娇^#,曹威,张清彬^*,崔诗曼,黎星阳

广州医科大学附属口腔医院·广州口腔病研究所·口腔医学重点实验室广东广州 510140

收稿日期:2016-08-10 出版日期:2017-01-25 发布日期:2017-01-22
通讯作者: 张清彬,电话:18825085727
#为共同第一作者
作者简介:赖世翔(1991~ ),男,广东梅州五华人,硕士在读,主要从事材料免疫学方面的研究工作。
陈良娇(1985~ ),女,安徽人,博士,主治医师,主要从事口腔生物材料的基础研究与应用。
基金资助:
广东省科学技术厅项目(编号:2016ZC0147)
广东省科学技术厅项目(编号:2015110-13)
广州市荔湾区科技计划项目(编号:20151217094)
广州医科大学校级博士启动基金(编号:2015C43)

Effects of Dicalcium Silicate on Murine RAW 264.7 Macrophage Cell Line.

LAI Shi-xiang, CHEN Liang-jiao^#, CAO Wei, ZHANG Qing-bin^*, CUI Shi-man, LI Xing-yang

Key Laboratory of Oral Medicine,Guangzhou Institute of Oral Disease, Stomatological Hospital of Guangzhou Medical University, Guangzhou 510140, China

Received:2016-08-10 Online:2017-01-25 Published:2017-01-22

摘要/Abstract

摘要： 目的:探讨硅酸二钙对小鼠巨噬细胞系RAW264.7的促炎反应机制。方法:选取小鼠巨噬细胞系RAW264.7作为研究对象,磷酸三钙(Tricalcium phosphate,简称TCP)颗粒作为对比,硅酸二钙(Dicalcium silicate,简称C2S)、磷酸三钙颗粒均设置两种浓度:10 mg/L和100 mg/L,观察RAW264.7对两种材料的吞噬作用,观察是否有细胞自噬现象发生,采用qRT-PCR的方法,检测硅酸二钙、磷酸三钙颗粒分别与RAW264.7共培养6 h和24 h后TLR2(Toll like receptor2)、TLR9基因的表达量,初步探讨硅酸二钙促炎反应的机制。结果:硅酸二钙与磷酸三钙颗粒与RAW264.7共培养后,细胞吞噬颗粒,通过透射电镜观察可见细胞自噬现象。与磷酸三钙颗粒组相比,硅酸二钙颗粒组与RAW264.7共培养6 h后,TLR2、TLR9基因的表达均无明显变化;共培养24 h后,硅酸二钙颗粒组的TLR2基因表达量明显增高(P<0.001),10 mg/L磷酸三钙颗粒组的TLR2基因表达可能增高(0.05<P<0.1)。结论:硅酸二钙对小鼠巨噬细胞RAW264.7的促炎反应与TLR2基因的高表达可能有密切的关系。

关键词: 硅酸二钙, 磷酸三钙, 巨噬细胞, 促炎反应

Abstract: Objective: To investigate the proinflammatory response of dicalcium silicate on murine RAW 264.7 macrophage cell line. Methods: qRT-PCR method was used to determine the effects of dicalcium silicate and tricalcium phosphate particles on expressions of TLR2 and TLR9 co-cultured with RAW264.7 for 6 h and 24 h. Results: Scanning electron microscope revealed that RAW264.7cells phagocytosed both dicalcium silicate and tricalcium phosphate particles. Compared with tricalcium phosphate particle group, TLR2 and TLR9 showed no obvious change in dicalcium silicate particles group when co-cultured with RAW264.7 for 6 h. For 24 h, the expression of TLR2 in dicalcium silicate particle group was obviously higher (P<0.001), and the expression of TLR2 was also increased in 10 mg/L tricalcium phosphate group (0.05<P<0.1). Conclusion: TLR2 may be involved in the proinflammatory response of dicalcium silicate murine RAW 264.7 macrophage cell line.

Key words: Dicalcium, silicate, Tricalcium, phosphate, Macrophages, Proinflammatory, response

中图分类号:

R783.1

赖世翔,陈良娇,曹威,张清彬,崔诗曼,黎星阳. 硅酸二钙对小鼠巨噬细胞系RAW264.7的促炎反应机制研究[J]. 口腔医学研究, 2017, 33(1): 29-32.

LAI Shi-xiang, CHEN Liang-jiao, CAO Wei, ZHANG Qing-bin, CUI Shi-man, LI Xing-yang. Effects of Dicalcium Silicate on Murine RAW 264.7 Macrophage Cell Line.[J]. Journal of Oral Science Research, 2017, 33(1): 29-32.

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硅酸二钙对小鼠巨噬细胞系RAW264.7的促炎反应机制研究

Effects of Dicalcium Silicate on Murine RAW 264.7 Macrophage Cell Line.

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