口腔医学研究

口腔医学研究 ›› 2018, Vol. 34 ›› Issue (12): 1333-1336.DOI: 10.13701/j.cnki.kqyxyj.2018.12.017

• 牙周病学研究 • 上一篇    下一篇

Mongersen在牙周炎症微环境中抗炎作用的体外研究

王柳然1,刘歆婵2,孟阳1,丁小函1,武洲3,于维先4*   

  1. 1. 吉林大学口腔医学院牙周病科 吉林 长春 130021;
    2. 吉林大学口腔医学院老年口腔科 吉林 长春 130021;
    3. 日本九州大学 大学院齿学研究院 日本 福冈 812-8581;
    4. 吉林大学口腔医学院 吉林省牙发育及颌骨重塑与再生重点实验室 吉林 长春 130021
  • 收稿日期:2018-06-10 出版日期:2018-12-28 发布日期:2018-12-27
  • 通讯作者: 于维先,E-mail: yu-wei-xian@163.com
  • 作者简介:王柳然(1993~ ),女,吉林人,硕士,医师,主要从事牙周免疫炎症的研究。
  • 基金资助:
    国家自然科学基金面上项目(编号:81570983)吉林省卫生技术创新项目(编号:2016J073)吉林省科技厅国际合作项目(编号:20180414053GH)

Study on Mongersen's Anti-inflammatory Effect in Periodontal Inflammatory Microenvironment in Vitro.

WANG Liu-ran1, LIU Xin-chan2, MENG Yang1, DING Xiao-han1, WU Zhou3, YU Wei-xian4*   

  1. 1. Department of Periodontics, School of Stomatology, Jilin University, Changchun 130021, China;
    2. Department of Dental Implantology, School of Stomatology, Jilin University, Changchun 130021, China;
    3. Institute of Dentistry, University of Kyushu, Fukuoka-ken 812-8581, Japan;
    4. Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, School of Stomatology, Jilin University, Changchun 130021, China.
  • Received:2018-06-10 Online:2018-12-28 Published:2018-12-27

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摘要： 目的: 探讨Smad7反义寡核苷酸mongersen的体外抗炎作用。方法: 使用LipofectamineTM，2000载mongersen转染小鼠巨噬细胞RAW264.7,之后将RAW264.7与牙周炎的主要致病菌牙龈卟啉单胞菌的脂多糖(Porphyromonas gingivlis lipopolysaccharide,Pg-LPS)共培养,以模拟牙周炎症微环境,共培养12 h后,采用流式细胞术、Western blot、qRT-PCR分别检测转染效率、Smad7蛋白的表达情况及巨噬细胞相关细胞因子的表达。结果: 流式细胞术检测转染效率可达76.57%;Western blot结果显示mongersen可明显抑制Smad7蛋白的表达;qRT-PCR结果显示加入mongersen后肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素-6(interleukin-6,IL-6)、白细胞介素-1β(interleukin-1β,IL-1β)等促炎因子基因表达明显降低。结论: mongersen可通过抑制Smad7蛋白的表达发挥抗炎作用。

关键词: Mongersen, Smad7, 巨噬细胞, 牙周炎

Abstract: Objective: To investigate the anti-inflammatory effect of mongersen, a Smad7 antisense oligonucleotide,in vitro.Methods: Lipofectamine TM 2000 contained mongersen was used to tansfect mouse macrophage (RAW264.7).Then RAW264.7 cells were co-cultured with Pg-LPS which was the main pathogen of periodontitis to simulate the periodontal inflammatory environment.After 12 hours, flow cytometry, western blot, and qRT-PCR were used to detect the transfection efficiency and expression of Smad7 protein and macrophages-related cytokines.Results: The transfection efficiency was 76.57% by flow cytometry.Mongeren could inhibit the protein expression of Smad7 by western blot.The expression of TNF-a, IL-6, IL-1β, and other pro-inflammatory cytokines were significantly reduced after adding mongersen.

Key words: Mongersen, Smad7, Macrophage, Periodontitis