增强AhR表达对高糖状态牙龈卟啉单胞菌脂多糖导致的巨噬细胞炎症反应的影响

doi:10.13701/j.cnki.kqyxyj.2018.12.016

口腔医学研究 ›› 2018, Vol. 34 ›› Issue (12): 1329-1332.DOI: 10.13701/j.cnki.kqyxyj.2018.12.016

增强AhR表达对高糖状态牙龈卟啉单胞菌脂多糖导致的巨噬细胞炎症反应的影响

钟星华¹,李昊^1*,李伟²

1. 广西医科大学附属口腔医院口腔修复科广西南宁 530021;
2. 四川大学华西口腔医学院口腔疾病研究国家重点实验室四川成都 610041

收稿日期:2018-05-17 出版日期:2018-12-28 发布日期:2018-12-27
通讯作者: 李昊,E-mail:sherrylee2011@126.com
作者简介:钟星华(1985~ ),女,广西玉林人,主治医师,主要从事口腔修复学基础与临床研究。
基金资助:
国家自然科学基金(编号:81600833)广西自然科学基金(编号:2016GXNSFAA380168)

Effects of AhR Overexpression on the Porphyromonas gingivalis Lipopolysaccharide- caused Inflammatory Response of Macrophages under High Glucose Conditions.

ZHONG Xing-hua¹, LI Hao^1*, LI Wei²

1. Department of Prothodontics, the Affiliated Hospital of Stomatology, Guangxi Medical University, Nanning 530021, China;
2. State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.

Received:2018-05-17 Online:2018-12-28 Published:2018-12-27

摘要/Abstract

摘要： 目的: 探讨增强芳香烃受体(AhR)对高糖状态牙龈卟啉单胞菌(Pg)脂多糖(LPS)导致的巨噬细胞炎症反应的影响。方法: 将小鼠巨噬细胞RAW264.7接种在高糖培养基中,并转染特异性增强AhR表达的重组质粒,稳定转染后将细胞在高糖状态加入Pg LPS刺激。用免疫印迹实验检测细胞的AhR及炎症反应相关蛋白Notch1、Jagged1、iNOS的表达,并用ELISA检测细胞培养上清液中的促炎因子TNFα、IL1β浓度。结果: 在受高糖加Pg LPS刺激后,与未增强AhR表达的对照组细胞相比,增强AhR表达的细胞Notch1、Jagged1、iNOS、TNFα、IL1β蛋白表达量减少(P<0.05)。结论: 增强AhR表达,可抑制高糖状态Pg LPS导致的巨噬细胞炎症反应,该抑制作用可能与AhR调节Notch通路有关。

关键词: 芳香烃受体, 牙龈卟啉单胞菌, 巨噬细胞, 炎症反应, 高糖状态

Abstract: Objective: To investigate the effects of enhanced expression of aryl hydrocarbon receptor (AhR) on the inflammatory response of macrophages induced by Porphyromonas gingivalis lipopolysaccharide (Pg LPS) under high glucose conditions.Methods: RAW264.7 murine macrophages were inoculated in the medium with high glucose and were transfected with recombinant plasmids to enhance AhR expression.Then, Pg LPS was added to the medium with high glucose to induce inflammatory response.Western blotting was chosen to examine the expression of AhR, Notch1, Jagged1 and iNOS in macrophages, and ELISA was used to detect the concentration of TNFα and IL1β in the culture supernatant.Results: Compared with the cells without enhanced AhR expression, the cells overexpressing AhR exhibited less expression of Notch1, Jagged1, iNOS, TNFα, and IL1β under the condition of Pg LPS stimulation superimposed on high glucose (P<0.05).Conclusion: Increased AhR expression can inhibit the inflammatory response of macrophages induced by Pg LPS under high glucose conditions, and the suppresive effect may be associated with the inhibition of Notch signaling by AhR.

Key words: Aryl hydrocarbon receptor, Porphyromonas gingivalis, Macrophage, Inflammatory responseHigh glucose

钟星华,李昊,李伟. 增强AhR表达对高糖状态牙龈卟啉单胞菌脂多糖导致的巨噬细胞炎症反应的影响[J]. 口腔医学研究, 2018, 34(12): 1329-1332.

ZHONG Xing-hua, LI Hao, LI Wei. Effects of AhR Overexpression on the Porphyromonas gingivalis Lipopolysaccharide- caused Inflammatory Response of Macrophages under High Glucose Conditions.[J]. Journal of Oral Science Research, 2018, 34(12): 1329-1332.

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增强AhR表达对高糖状态牙龈卟啉单胞菌脂多糖导致的巨噬细胞炎症反应的影响

Effects of AhR Overexpression on the Porphyromonas gingivalis Lipopolysaccharide- caused Inflammatory Response of Macrophages under High Glucose Conditions.

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