水溶性石墨烯转染滑膜间充质干细胞的研究

doi:10.13701/j.cnki.kqyxyj.2017.04.001

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (4): 349-352.DOI: 10.13701/j.cnki.kqyxyj.2017.04.001

• 基础研究论著 • 下一篇

水溶性石墨烯转染滑膜间充质干细胞的研究

王玥, 邵博, 龚忠诚^*, 杨萌, 胡鑫, 刘慧, 胡露露, 王冰

新疆医科大学第一附属医院颌面肿瘤外科,新疆医科大学口腔医学院,新疆维吾尔自治区口腔医学研究所新疆乌鲁木齐 830054

收稿日期:2016-09-14 出版日期:2017-04-20 发布日期:2017-04-24
通讯作者: 龚忠诚,电话:13319822466
作者简介:王玥(1989~ ),新疆石河子人,硕士在读,主要从事颌面肿瘤外科的研究工作。
基金资助:
国家自然科学基金项目(编号:31260229),新疆维吾尔自治区优秀青年科技创新人才培养项目(编号:2014721046)

Transfection Efficiency of Water Soluble Grapheme on Synovial Stem Cells.

WANG Yue, SHAO Bo, GONG Zhong-cheng^*, YANG Meng, HU Xin, LIU Hui, HU Lu-lu, WANG Bing.

Department of Maxillofacial Tumors Surgery, The First Affiliated Hospital of Xinjiang Medical University; School of Stomatology, Xinjiang Medical University;Oral Medical Research Institute of Xinjiang Uygur Autonomous Region. Urumqi 830054, China.

Received:2016-09-14 Online:2017-04-20 Published:2017-04-24

摘要/Abstract

摘要： 目的:探究水溶性石墨烯转染滑膜间充质干细胞的的效果及各组间细胞克隆形成率的差别比较。方法:将传代培养到第3代的滑膜间充质干细胞分成实验组与对照组,实验组细胞培养基内加入浓度为25 mol/mL的水溶性石墨烯在37 ℃的条件下培养24 h,对照组用普通培养基培养37 ℃ 24 h,405 nm激发波长下的激光共聚胶显微镜下观察石墨烯是否成功转染滑膜干细胞。各组间细胞克隆形成率的差别采用单因素方差分析比较。结果:实验组的细胞培养24 h后,在激光共聚焦显微镜下细胞质可见发光的荧光点,细胞核的光强度较弱。各组克隆形成率均P<0.05有显著差异。结论:石墨烯容易渗透到细胞质但没有进入细胞核,可以猜测石墨烯在进入活的滑膜间充质干细胞后没有发生遗传的中断。

Abstract: Objective:To study the transfection efficiency of water soluble grapheme on synovuial stem cells. Methods: The third generation of synovial-derived mesenchymal stem cells (SMSCs) was divided into two groups. In experimental group, cells were cultured with the concentration of 25moL/mL water soluble grapheme for 24 hours at the temperature of 37℃. For the control group, cells were cultured with common medium for 24 hours at the temperature of 37℃. The laser scanning microscope with 405nm excitation wavelength was employed to detect the cells marked by the water soluble grapheme. Results: Fluorescence spots could be observed in the cytoplasmic area of these stem cells in experimental group 24 hours later under the laser scanning microscope, and the light intensity of nucleus was relatively weak. Conclusion: Grapheme quantum dots (GQDs) are easily penetrated into the cytoplasm but not the nucleus. It is suggested that genetic disruptions might not happen after the GQDs entered the living stem cells.

Key words: SMSCs , GQDs , Tissue engineering, Cell cloning

中图分类号:

R329.2

王玥, 邵博, 龚忠诚, 杨萌, 胡鑫, 刘慧, 胡露露, 王冰. 水溶性石墨烯转染滑膜间充质干细胞的研究[J]. 口腔医学研究, 2017, 33(4): 349-352.

WANG Yue, SHAO Bo, GONG Zhong-cheng, YANG Meng, HU Xin, LIU Hui, HU Lu-lu, WANG Bing.. Transfection Efficiency of Water Soluble Grapheme on Synovial Stem Cells.[J]. Journal of Oral Science Research, 2017, 33(4): 349-352.

参考文献

[1] 芮云峰,林禹丞,陈辉,等.晚期骨关节炎患者膝关节滑膜间充质干细胞的体外成骨分化[J].中国组织工程研究,2013(45)∶7840-7846
[2] 邵博,胡露露,龚忠诚,等.滑膜间充质干细胞与软骨细胞接触式共培养构建软骨的初步研究[J].口腔医学研究,2016(06)∶554-558
[3] Futami I, Ishijima M, Kaneko H, et al. Isolation and characterization of multipotential mesenchymal cells from the mouse synovium [J]. PLoS One,2012,7(9)∶e45517
[4] Levorson EJ, Santoro M, Kurtis Kasper F, et al. Direct and indirect co-culture of chondrocytes and mesenchymal stem cells for the generation of polymer/extracellular matrix hybrid constructs [J]. Acta Biomaterialia,2014,10(5)∶1824-1835
[5] Liu Y, Chan JKY, Teoh S. Review of vascularised bone tissue-engineering strategies with a focus on co-culture systems [J]. Journal of Tissue Engineering and Regenerative Medicine,2015,9(2)∶85-105
[6] Li S, Li Y, Cao J, et al. Sulfur-Doped Graphene Quantum Dots as a Novel Fluorescent Probe for Highly Selective and Sensitive Detection of Fe3+ [J]. Analytical Chemistry,2014,86(20)∶10201-10207
[7] Fan Z, Li Y, Li X, et al. Surrounding media sensitive photoluminescence of boron-doped graphene quantum dots for highly fluorescent dyed crystals, chemical sensing and bioimaging [J]. Carbon,2014,70(1)∶149-156
[8] Zhang M, Bai L, Shang W, et al. Facile synthesis of water-soluble, highly fluorescent graphene quantum dots as a robust biological label for stem cells [J]. Journal of Materials Chemistry,2012,22(15)∶7461-7468
[9] Tan X, Li Y, Li X, et al. Electrochemical synthesis of small-sized red fluorescent graphene quantum dots as a bioimaging platform [J]. Chem. Commun.,2015,51(13)∶2544-2546
[10] 敏世雄,吕功煊.CdS/石墨烯复合材料的制备及其可见光催化分解水产氢性能[J].物理化学学报,2011(09)∶2178-2184
[11] 匡达,胡文彬.石墨烯复合材料的研究进展[J].无机材料学报,2013(03)∶235-246

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水溶性石墨烯转染滑膜间充质干细胞的研究

Transfection Efficiency of Water Soluble Grapheme on Synovial Stem Cells.

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