口腔医学研究

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (7): 703-706.DOI: 10.13701/j.cnki.kqyxyj.2017.07.004

• 基础研究论著 • 上一篇    下一篇

纯钛微弧氧化-多巴胺-环型多肽涂层的细胞相容性研究

周田园1, 李德超1*, 李慕勤1*, 李善昌2, 刘爽2, 马菁2, 田垒3, 于佳莹3, 温鑫3   

  1. 佳木斯大学 黑龙江省高校生物医学材料重点实验室 黑龙江 佳木斯 154007
  • 收稿日期:2016-12-05 出版日期:2017-07-20 发布日期:2017-07-27
  • 通讯作者: 李德超,电话:0454-8625875李慕勤,电话:0454-8618700
  • 作者简介:周田园(1991~ ),女,江苏省徐州市人,硕士,主要从事牙周种植学的研究工作。
  • 基金资助:
    国家自然科学基金项目(编号:31370979)佳木斯大学大学生科技创新项目(编号:xsym 2016-07)佳木斯大学口腔医学预防与治疗协同创新中心(编号:2011xtcx 2016-02)

Cytocompatibility of Ultrasonic Micro-arc Oxidation-Dopamine-Cyclo (RGD) Coating on Pure Titanium.

ZHOU Tian-yuan1, LI De-chao1*, Li Mu-qin1*, Li Shan-chang2, Liu Shuang2, Ma Jing2, Tian Lei3, Yu Jia-ying3, Wen Xin3.   

  1. Key Biomedical Materials Laboratory of Colleges and Universities in Heilongjiang Province, Jiamusi University, Jiamusi 154007, China
  • Received:2016-12-05 Online:2017-07-20 Published:2017-07-27

PDF (PC)

189

被引次数

2

摘要： 目的:探讨纯钛种植体表面加载环型多肽对成骨细胞生物行为的影响。方法:微弧氧化组为M组,微弧氧化加载多巴胺组为P组,微弧氧化加载多巴胺接枝环环肽组为R组。扫描电镜对3组钛片进行表面形貌分析,能谱仪分析3组试件元素成分。CCK-8检测成骨细胞不同时间点在3组的增殖黏附能力,激光共聚焦观察成骨细胞在3组涂层表面的铺展情况。结果:扫描电镜及能谱显示涂层加载成功,CCK-8显示R组的A值在各个时间点上都高于M组和P组,且各组比较具有统计学意义(P<0.05)。激光共聚焦观察R组细胞数目最多,伪足较多,细胞联系最为紧密。结论:纯钛表面加载环肽后能有效的促进成骨细胞早期的黏附及增殖。

关键词: 钛, 微弧氧化, 多巴胺, 环肽

Abstract: Objective: To discuss the effect of Cyclo (RGD) coating on the surface of pure titanium implant on the biological behavior of osteoblasts. Methods: The specimens were divided into 3 groups: pure titanium ultrasonic micro-arc-oxidation (group M); pure titanium ultrasonic micro-arc-oxidation-dopamine (group P); and pure titanium ultrasonic micro-arc-oxidation-dopamine-Cyclo (RGD) (group R). Scanning electron microscope was used to observe the surface morphology. The surface elements of three groups were analyzed by energy spectrometer. CCK-8 experiment was used to detect the adhesion rate and proliferation of osteoblasts in different time points. The spread of osteoblasts on the surface of three groups was observed by laser confocal microscopy. Results: The observation of scanning electron microscope and the spectrum of coating displayed successful loading. And the results of CCK-8 showed that the OD value of group R was higher than those of group M and group P on tested time points (P<0.05). Confocal laser microscope showed the cytoskeleton form. During the same period, the spread area and the number of microfilament and pseudopodia of both group P and group R were better than those of group M. And the group R was the most conducive to cell stretch. Conclusion: The ultrasonic micro-arc oxidation-dopamine-cyclo (RGD) coating showed a good effect on early adhesion and proliferation of osteoblasts.

Key words: Pure titanium , Micro-arc oxidation, Dopamine , Cyclo (RGD)

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