抑制白色念珠菌用载氟康唑明胶纤维支架的制备及表征

doi:10.13701/j.cnki.kqyxyj.2018.04.023

口腔医学研究 ›› 2018, Vol. 34 ›› Issue (4): 437-441.DOI: 10.13701/j.cnki.kqyxyj.2018.04.023

抑制白色念珠菌用载氟康唑明胶纤维支架的制备及表征

周阳, 李琛^*, 高旭, 马晓周, 刘畅, 薛晓寒, 魏秀峰^*

吉林大学口腔医院黏膜病科,吉林省牙发育及颌骨重塑与再生重点实验室吉林长春 130021

收稿日期:2017-10-22 出版日期:2018-04-28 发布日期:2018-04-25
通讯作者: 李琛,E-mail:lichen@jlu.edu.cn魏秀峰,E-mail:xfw79@sina.cn
作者简介:周阳(1991~ ),女,吉林省白城市人,硕士在读,主要从事口腔黏膜病基础与临床方面的研究。
基金资助:
国家自然基金青年科学基金(编号:81500820)吉林省科技厅资助课题(编号:20160101052JC)吉林大学白求恩医学部青年基金(编号:2015431)

Preparation and Characterization of Fluconazole-loaded Gelatin Fiber Scaffolds for Inhibiting Candida Albicans.

ZHOU Yang, LI Chen^*, GAO Xu, MA Xiao-zhou, LIU Chang, XUE Xiao-han, WEI Xiu-feng^*

Department of Oral Medicine and Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, School and Hospital of Stomatology, Jilin University, Changchun 130021, China.

Received:2017-10-22 Online:2018-04-28 Published:2018-04-25

摘要/Abstract

摘要： 目的:制备载氟康唑明胶纤维支架,作为抑制白色念珠菌生长的新型制剂,探讨其理化性能及生物性能。方法:采用静电纺丝方法制备载氟康唑明胶纤维支架,在扫描电镜下观察其形貌表征;通过体外降解实验测试其稳定性;紫外分光光度检测氟康唑释放量;细胞黏附实验观察其黏附性;MTT检测生物安全性;体外培养白色念珠菌测试其抑制白色念珠菌性能。结果:载氟康唑明胶纤维支架具有纳米级纤维直径,呈交错的三维网络结构,5 d时体外降解量约为10%;细胞存活率高,部分细胞黏附在支架表面,部分伸入其中;氟康唑在6 h内释放较快,此后缓慢释放;支架材料对白色念珠菌具有抑制作用。结论:载氟康唑明胶纤维支架的稳定性、黏附性及生物安全性好,药物释放量大而持久,抑制白色念珠菌性能优异。

关键词: 明胶纤维支架, 氟康唑, 白色念珠菌

Abstract: Objective: To develop fluconazole-loaded gelatin fiber scaffolds for inhibiting the growth of candida albicans, and to explore their physicochemical properties and biological properties. Methods: The fluconazole-loaded gelatin fiber scaffolds were prepared by electrospinning, and the morphology was observed by SEM. The amount of fluconazole release was detected by UV spectrophotometry. The adhesion property was observed by cell adhesion assay and the biosafety was detected by MTT. Candida albicans were cultured in vitro to test its inhibitory effect on candida albicans. Results: The fluconazole-loaded gelatin fiber scaffolds exhibited nanoscale diameter with interlaced three-dimensional network structures, and the degradation rate in vitro was about 10% after 5 days. The cell viability was high. Some cells adhered to the surface of scaffolds, and the others extended into it. Fluconazole released fast within the first 6 hours, then got slow. Scaffolds had inhibitory effect on candida albicans. Conclusion: Fluconazole-loaded gelatin fiber scaffolds have good stability, adhesion, and biosafety with large and lasted drug release ability. The inhibitory effect on candida albicans is excellent.

Key words: Gelatin fiber scaffolds, Fluconazole, Candida albicans

周阳, 李琛, 高旭, 马晓周, 刘畅, 薛晓寒, 魏秀峰. 抑制白色念珠菌用载氟康唑明胶纤维支架的制备及表征[J]. 口腔医学研究, 2018, 34(4): 437-441.

ZHOU Yang, LI Chen, GAO Xu, MA Xiao-zhou, LIU Chang, XUE Xiao-han, WEI Xiu-feng. Preparation and Characterization of Fluconazole-loaded Gelatin Fiber Scaffolds for Inhibiting Candida Albicans.[J]. Journal of Oral Science Research, 2018, 34(4): 437-441.

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抑制白色念珠菌用载氟康唑明胶纤维支架的制备及表征

Preparation and Characterization of Fluconazole-loaded Gelatin Fiber Scaffolds for Inhibiting Candida Albicans.

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