Er:YAG激光对牙本质与通用粘接剂粘接界面特征的影响

doi:10.13701/j.cnki.kqyxyj.2021.10.017

摘要/Abstract

摘要： 目的:观察Er:YAG激光预处理的牙本质与通用粘接剂在自酸蚀和酸蚀-冲洗使用模式下粘接界面的超微结构特征,并与未处理的牙本质粘接界面进行对比,以探索二者之间粘接机制的差异。方法:选取36颗完整的人类第三磨牙,按照表面预处理方式和通用粘接剂的使用模式随机分为4组(A组:空白对照+自酸蚀;B组:空白对照+酸蚀-冲洗(35%磷酸);C组:Er:YAG激光+自酸蚀; D组:Er:YAG激光+酸蚀-冲洗)。每组9颗牙齿分为3组,分别制备成3 mm厚牙本质盘、2 mm厚的树脂-牙本质粘接界面纵断面样本和树脂-牙本质粘接剂面透光磨片。然后用扫描电镜(SEM)和X射线能谱仪(EDX)进行界面观察和牙本质表面钙(Ca)和磷(P)的质量分数分析,用激光共聚焦扫描显微镜(CLSM)观察树脂突的长度和数量。结果:SEM和EDX分析结果显示Er:YAG激光预处理改变了牙本质表面形貌,增加了牙本质表面Ca的质量分数。C组粘接界面可见与B、D组相似厚度的高密度混合层。A、B和D组粘接界面可见高密度层。C组的树脂突长度和数量均高于A组。结论:Er:YAG激光预处理后的牙本质与通用粘接剂在自酸蚀模式下粘接界面形成了高密度的特殊混合层,并且增加了树脂突的长度和数量。

关键词: Er:YAG激光, 牙本质, 通用粘接剂, 树脂-牙本质界面

Abstract: Objective: To observe the characteristics of resin/dentin interface between dentine pretreated by Er:YAG laser and universal adhesive with self-etching or etch-and-rinse mode, and to explore the differences between two bonding mechanisms through comparing with traditional treated dentine bonding interface. Methods: Thirty-six intact human third molars were selected and randomly divided into four groups (group A: control + self-etching; group B: control + 35% phosphoric acid etching; group C: Er:YAG laser + self-etching; group D: Er:YAG laser+ 35% phosphoric acid etching). Nine teeth in each group were divided into three groups, which were prepared dentin disk in the thickness of 3mm and longitudinal cross-section of resin-dentin interface and resin-dentin transparent grinding plates in the thickness of 2mm. The interface observation and mass fraction analysis of calcium (Ca) and phosphorus (P) on dentine surface were carried out by scanning electron microscope (SEM) and energy-dispersive X-ray spectroscopy (EDX). The length and number of resin tags were observed by laser confocal scanning microscope (CLSM). Results: SEM and EDX analysis showed that the surface of dentine was changed by Er:YAG laser pretreatment, and the mass fraction of Ca was increased. The bonding interface of group C showed a high density hybrid layer with similar thickness to that of group B and D. High density layer could be seen at the bonding interface of group A, B, and D. The length and number of resin tags in group C were higher than those in group A. Conclusion: In the self-etching mode, the dentine pretreated by Er:YAG laser and the universal adhesive formed a special hybrid layer with high density, which increased the length and number of resin tags.

Key words: Er:YAG laser, dentin, universal adhesive, resin-dentin interface

李振杰, 周伯阳, 郝鹏杰. Er:YAG激光对牙本质与通用粘接剂粘接界面特征的影响[J]. 口腔医学研究, 2021, 37(10): 940-944.

LI Zhenjie, ZHOU Boyang, HAO Pengjie. Effect of Er:YAG Laser on Interface of Dentin and Universal Adhesive[J]. Journal of Oral Science Research, 2021, 37(10): 940-944.

参考文献

[1] Keller U, Raab WH, Hibst R. Pulp reactions during Erbium YAG laser irradiation of hard tooth structure [J]. Dtsch Zahnarztl Z, 1991, 46(2):158-160.
[2] 黄翠,刘英衡.口腔粘接与粘固的区别和联系[J].口腔医学研究,2021,37(5):381-385.
[3] Zhang Y, Jiang A. The influence of Er:YAG laser treatment on the shear bond strength of enamel and dentin: a systematic review and meta-analysis [J]. Quintessence Int, 2020, 51(1):8-16.
[4] Cersosimo MC, Matos AB, Couto RS, et al. Short-pulse Er:YAG laser increases bond strength of composite resin to sound and eroded dentin [J]. J Biomed Opt, 2016, 21(4):48001.
[5] Guven Y, Aktoren O. Shear bond strength and ultrastructural interface analysis of different adhesive systems to Er:YAG laser-prepared dentin [J]. Lasers Med Sci, 2015, 30(2):769-778.
[6] Bahrololoomi Z, Kabudan M, Gholami L. Effect of Er:YAG laser on shear bond strength of composite to enamel and dentin of primary teeth [J]. J Dent (Tehran), 2015, 12(3):163-170.
[7] Karadas M, Çalari. The effect of Er:YAG laser irradiation on the bond stability of self-etch adhesives at different dentin depths [J]. Lasers Med Sci, 2017, 32(5):967-974.
[8] Dönmez N, Güngör AS, Karabulut B, et al. Comparison of the micro-tensile bond strengths of four different universal adhesives to caries-affected dentin after ER:YAG laser irradiation [J]. Dent Mater J, 2019, 38(2):218-225.
[9] Ghiggi PC, Dall Agnol RJ, Burnett LH Jr, et al. Effect of the Nd:YAG and the Er:YAG laser on the adhesive-dentin interface: a scanning electron microscopy study [J]. Photomed Laser Surg, 2010, 28(2):195-200.
[10] Koliniotou-Koumpia E, Kouros P, Zafiriadis L, et al. Bonding of adhesives to Er:YAG laser-treated dentin [J]. Eur J Dent,2012,6(1):16-23.
[11] Sarr M, Kane AW, Vreven J, et al. Microtensile bond strength and interfacial characterization of 11 contemporary adhesives bonded to bur-cut dentin [J]. Oper Dent, 2010, 35(1):94-104.
[12] Chowdhury AFMA, Alam A, Yamauti M, et al. Characterization of an experimental two-step self-etch adhesive's bonding performance and resin-dentin interfacial properties [J]. Polymers (Basel), 2021, 13(7):1009.
[13] Shadman N, Farzin Ebrahimi S, Amanpour S, et al. Shear bond strength of a multi-mode adhesive to bur-cut and Er,Cr:YSGG lased dentin in different output powers [J]. J Dent (Shiraz), 2019, 20(2):118-123.
[14] Yoshida Y, Nagakane K, Fukuda R, et al. Comparative study on adhesive performance of functional monomers [J]. J Dent Res, 2004, 83(6):454-458.
[15] Trevelin LT, da Silva BTF, de Freitas PM, et al. Influence of Er:YAG laser pulse duration on the long-term stability of organic matrix and resin-dentin interface [J]. Lasers Med Sci, 2019, 34(7):1391-1399.
[16] Yoshihara K, Nagaoka N, Hayakawa S, et al. Chemical interaction of glycero-phosphate dimethacrylate (GPDM) with hydroxyapatite and dentin [J]. Dent Mater, 2018, 34(7):1072-1081.
[17] Takamizawa T, Imai A, Hirokane E, et al. SEM observation of novel characteristic of the dentin bond interfaces of universal adhesives [J]. Dent Mater, 2019, 35(12):1791-1804.
[18] Aljdaimi A, Devlin H, Dickinson M, et al. Effect of 2.94 μm Er: YAG laser on the chemical composition of hard tissues [J]. Microsc Res Tech, 2018, 81(8):887-896.
[19] Anchieta RB, Oliveira FG, Sundfeld RH, et al. Analysis of hybrid layer thickness, resin tag length and their correlation with microtensile bond strength using a total etch adhesive to intact dentin [J]. Acta Odontol Latinoam, 2011, 24(3):272-278.
[20] Lohbauer U, Nikolaenko SA, Petschelt A, et al. Resin tags do not contribute to dentin adhesion in self-etching adhesives [J]. J Adhes Dent, 2008, 10(2): 97-103.
[21] Tsujimoto A, Shimatani Y, Nojiri K, et al. Influence of surface wetness on bonding effectiveness of universal adhesives in etch-and-rinse mode [J]. Eur J Oral Sci, 2019, 127(2):162-169.
[22] Zhang Z, Beitzel D, Mutluay M, et al. On the durability of resin-dentin bonds: Identifying the weakest links [J]. Dent Mater, 2015, 31(9):1109-1118.