牙龈卟啉单胞菌GroEL对人脐静脉内皮细胞影响的研究

doi:10.13701/j.cnki.kqyxyj.2022.09.016

口腔医学研究 ›› 2022, Vol. 38 ›› Issue (9): 872-876.DOI: 10.13701/j.cnki.kqyxyj.2022.09.016

牙龈卟啉单胞菌GroEL对人脐静脉内皮细胞影响的研究

闫锐^1,2, 郭凯利^1,2, 曹丰弟^1,3, 张军¹, 马哲^1*

1. 河北医科大学口腔医学院·口腔医院河北石家庄 050017;
2. 河北省口腔医学重点实验室河北石家庄 050017;
3. 墨尔本大学牙学院澳大利亚墨尔本 VIC 3010

收稿日期:2022-02-18 发布日期:2022-09-26
通讯作者: *马哲,E-mail: mazhe2009@126.com
作者简介:闫锐(1997~ ),女,山东济宁人,硕士在读,主要从事牙周炎与心血管疾病的基础研究。
基金资助:
京津冀医疗卫生协调发展和公立医院改革能力建设(编号:2019061636)

Effects of Porphyromonas Gingivalis GroEL on Human Umbilical Vein Endothelial Cells.

YAN Rui^1,2, GUO Kaili^1,2, CAO Fengdi^1,3, ZHANG Jun¹, MA Zhe^1*

1. School and Hospital of Stomatology, Shijiazhuang 050017, China;
2. Hebei Key Laboratory of Stomatology, Hebei Medical Uiversity, Shijiazhuang 050017, China;
3. The University of Melbourne of Dentistry, Melbourne VIC 3010, Australia.

Received:2022-02-18 Published:2022-09-26

摘要/Abstract

摘要： 目的: 探究牙龈卟啉单胞菌(Porphyromonas gingivalis,P.g)的代谢产物GroEL对体外培养的人脐静脉内皮细胞(HUVEC)增殖活性、凋亡及炎症反应的影响,以期为探讨P.g致心血管疾病的发病机制提供理论依据。方法: 用不同浓度的P.g GroEL(2、4、6、8、10 μg/mL)处理HUVEC 24 h,观察处理前后细胞形态的变化。通过CCK-8法检测HUVEC的增殖活性,流式细胞学检测细胞凋亡,RT-qPCR和ELISA法分别检测白细胞介素-6(IL-6)、白细胞介素-8(IL-8)、细胞间粘附分子-1(ICAM-1)、血管细胞粘附因子-1(VCAM-1)、E-选择素(E-selectin)的表达。结果: P.g GroEL处理HUVEC 24 h后,显著降低了细胞活力并增加了细胞凋亡率(P＜0.05),且随着P.g GroEL浓度的增加,对细胞活性的抑制作用和细胞凋亡数量也逐渐增加。P.g GroEL还显著刺激了HUVEC分泌IL-6、IL-8、ICAM-1、VCAM-1、E-selectin(P＜0.05)。结论: P.g GroEL以浓度依赖性方式抑制HUVEC活性并诱导细胞发生凋亡。P.g GroEL还能引起HUVEC的炎症反应,从而在牙周炎促进动脉粥样硬化的发生中发挥重要作用。

关键词: 牙龈卟啉单胞菌, GroEL, 牙周炎, 人脐静脉内皮细胞, 动脉粥样硬化

Abstract: Objective: To investigate the effects of GroEL, a metabolite of Porphyromonas gingivalis(P.g) , on the proliferation viability, apoptosis, and inflammation response of human umbilical vein endothelial cell (HUVEC) in vitro. Methods: The HUVEC were stimulated with different concentrations of P.g GroEL (2, 4, 6, 8, 10 μg/mL) for 24 h to observe the morphologic changes. The proliferation viability of HUVEC was detected by CCK-8. Apoptosis rate of HUVEC was detected by flow cytometry. The expressions of interleukin-6 (IL-6), interleukin-8 (IL-8), intracellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and E-selectin were detected by RT-qPCR and ELISA, respectively. Results: P.g GroEL significantly decreased HUVEC viability, and apoptosis rate was increased at 24 h (P<0.05). The inhibitory effect of P.g GroEL and apoptosis rate was more obvious as the concentration increased. P.g GroEL also promoted the expression of IL-6, IL-8, ICAM-1, VCAM-1, and E-selectin (P<0.05). Conclusion: P.g GroEL may play an important role in the development of atherosclerosis promoted by periodontitis.

Key words: Porphyromonas gingivalis, GroEL, periodontitis, human umbilical vein endothelial cell, atherosclerosis

闫锐, 郭凯利, 曹丰弟, 张军, 马哲. 牙龈卟啉单胞菌GroEL对人脐静脉内皮细胞影响的研究[J]. 口腔医学研究, 2022, 38(9): 872-876.

YAN Rui, GUO Kaili, CAO Fengdi, ZHANG Jun, MA Zhe. Effects of Porphyromonas Gingivalis GroEL on Human Umbilical Vein Endothelial Cells.[J]. Journal of Oral Science Research, 2022, 38(9): 872-876.

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牙龈卟啉单胞菌GroEL对人脐静脉内皮细胞影响的研究

Effects of Porphyromonas Gingivalis GroEL on Human Umbilical Vein Endothelial Cells.

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