雷奈酸锶对大鼠伴糖尿病牙周炎牙槽骨吸收的影响

doi:10.13701/j.cnki.kqyxyj.2021.06.019

口腔医学研究 ›› 2021, Vol. 37 ›› Issue (6): 569-573.DOI: 10.13701/j.cnki.kqyxyj.2021.06.019

雷奈酸锶对大鼠伴糖尿病牙周炎牙槽骨吸收的影响

陈河林^1*, 王航², 黄卉³, 王宇¹, 熊玥³

1.贵州医科大学口腔医学院贵州贵阳 550000;
2.四川大学华西口腔医院四川成都 610000;
3.贵州医科大学附属口腔医院贵州贵阳 550000

收稿日期:2021-01-12 出版日期:2021-06-28 发布日期:2021-06-17
通讯作者: *陈河林,E-mail:helinchen0509@163.com
作者简介:陈河林(1990~ ),湖北恩施人,男,博士,研究方向:口腔修复学。
基金资助:
贵州医科大学国家自然科学基金培育项目(编号:19NSP043)

Effect of Strontium Ranelate on Alveolar Bone Loss in Diabetic Periodontitis in Rats

CHEN Helin^1*, WANG Hang², HUANG Hui³, WANG Yu¹, XIONG Yue³

1. School of Stomatology, Guizhou Medical University, Guiyang 550000, China;
2. West China Hospital of Stomatology, Sichuan University, Chengdu 610000, China;
3. The Affiliated Hospital of Stomatology, Guizhou Medical University, Guiyang 550000, China

Received:2021-01-12 Online:2021-06-28 Published:2021-06-17

摘要/Abstract

摘要： 目的:探讨雷奈酸锶对大鼠伴糖尿病牙周炎牙槽骨吸收的影响。方法:选取200 g左右雄性大鼠40只随机分为4组:糖尿病大鼠对照组(D)、糖尿病大鼠雷奈酸锶灌胃组(D+Sr)、糖尿病牙周炎组(D+L)、糖尿病牙周炎雷奈酸锶灌胃组(D+L+Sr),给药4周后处死,收集样本检测血清钙、磷和碱性磷酸酶浓度,牙槽骨吸收量和RANKL、OPG表达情况。结果:各组间血清钙和磷浓度无统计学差异(P＞0.05),但D+L+Sr组ALP浓度较D+L组高(P<0.05);颌骨三维重建后测得D+L+Sr组牙槽骨吸收量小于较D+L组(P<0.05);免疫组化染色结果RANKL/OPG比值D+L+Sr组小于D+L组。结论:雷奈酸锶可以通过OPG/RANKL途径调控牙槽骨的代谢,促进骨形成,抑制骨吸收,从而减少糖尿病状态下牙周炎牙槽骨的破坏。

关键词: 雷奈酸锶, 糖尿病, 牙周炎, 牙槽骨吸收, RANKL/OPG

Abstract: Objective: To investigate the effects of strontium ranelate on alveolar bone loss in diabetic periodontitis in rats. Methods: Forty male SD rats weighing 200g were adopted and divided into four groups randomly: group D (diabetic rats with no intervention); group D+Sr (diabetic rats with administration of strontium ranelate intragastrically); group D+L (diabetic rats with ligature); and group D+L+Sr (diabetic rats with ligature and administration of strontium ranelate intragastrically). After 1 month of strontium ranelate administration, rats were sacrificed and serum and the jaw were collected to measure the content of Ca, P, and alkaline phosphatase (ALP) as well as the alveolar bone loss and expression of RANKL and OPG. Results: The contents of Ca and P among the four groups had no significant difference (P＞0.05), while content of ALP in D+L+Sr group was significantly higher than that in D+L group (P<0.05). The three dimensional reconstruction image of the alveolar bone showed that alveolar bone loss in D+L+Sr group was smaller than that in D+L group. Ratio of RANKL/OPG in D+L+Sr group was samller than that in D+L group. Conclusion: Strontium ranelate might promote bone formation and inhibit bone resorption via regulation of RANKL/OPG axis and eventually ameliorate alveolar bone loss in diabetic periodontitis.

Key words: strontium ranelate, diabetes, periodontitis, alveolar bone loss, RANKL/OPG

陈河林, 王航, 黄卉, 王宇, 熊玥. 雷奈酸锶对大鼠伴糖尿病牙周炎牙槽骨吸收的影响[J]. 口腔医学研究, 2021, 37(6): 569-573.

CHEN Helin, WANG Hang, HUANG Hui, WANG Yu, XIONG Yue. Effect of Strontium Ranelate on Alveolar Bone Loss in Diabetic Periodontitis in Rats[J]. Journal of Oral Science Research, 2021, 37(6): 569-573.

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雷奈酸锶对大鼠伴糖尿病牙周炎牙槽骨吸收的影响

Effect of Strontium Ranelate on Alveolar Bone Loss in Diabetic Periodontitis in Rats

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