硝基油酸经Nrf2/HO-1通路对放射性损伤大鼠颌下腺上皮细胞的抗氧化损伤作用

doi:10.13701/j.cnki.kqyxyj.2022.12.008

口腔医学研究 ›› 2022, Vol. 38 ›› Issue (12): 1139-1144.DOI: 10.13701/j.cnki.kqyxyj.2022.12.008

硝基油酸经Nrf2/HO-1通路对放射性损伤大鼠颌下腺上皮细胞的抗氧化损伤作用

骆勤亮¹, 张霓霓¹, 龙远铸¹, 柏宇¹, 代敏¹, 唐建宏¹, 崔田宁¹, 黄桂林^2*

1.遵义医科大学附属口腔医院口腔颌面外科贵州遵义 563000;
2.遵义医科大学第五附属珠海医院口腔科广东珠海 519110

收稿日期:2022-05-23 出版日期:2022-12-28 发布日期:2022-12-26
通讯作者: *黄桂林,E-mail:chaojiehuanghg1@163.com
作者简介:骆勤亮(1994~ ),男,贵州人,硕士在读,研究方向:唾液腺的放射性损伤修复。
基金资助:
国家自然科学基金地区科学基金项目(项目批准号:81960204);贵州省教育厅青年科技人才成长项目(编号:黔教合KY[2022]291号);遵义市科技计划项目合同书[编号:遵义科核HZ字(2020)298号];遵义医科大学“未来临床名医”项目(编号:20211017)

Antioxidant Effects of Nitro-oleic Acid on Radiation-damaged Rat Submandibular Gland Epithelial Cells via Nrf2/HO-1 Pathway

LUO Qinliang¹, ZHANG Nini¹, LONG Yuanzhu¹, BAI Yu¹, DAI Min¹, TANG Jianhong¹, CUI Tianning¹, HUANG Guilin^2*

1. Department of Oral and Maxillofacial Surgery, Stomatological Hospital Affiliated to Zunyi Medical University, Zunyi 563000, China;
2. Department of Stomatology, The Fifth Affiliated Zhuhai Hospital of Zunyi Medical University, Zhuhai 519110, China

Received:2022-05-23 Online:2022-12-28 Published:2022-12-26

摘要/Abstract

摘要： 目的: 硝基油酸(Nitro-oleic acid,OA-NO2)能否经Nrf2/HO-1通路对放射性损伤大鼠颌下腺上皮细胞发挥抗氧化损伤作用。方法: 体外培养乳鼠3 d颌下腺上皮细胞经电子直线加速器建立放射性损伤模型,CCK-8法筛选OA-NO2作用24 h对放射性损伤细胞模型最佳增殖浓度,按实验目的分为正常细胞组(NG)、放射细胞组(RG)、放射细胞+硝基油酸组(R+ONG)、放射细胞+硝基油酸+抑制剂组(R+ON+BRG)、放射细胞+抑制剂组(R+BRG);各组细胞培养24 h后Western blot检测Nrf2、HO-1及NQO1蛋白的表达及活性氧试剂检测活性氧的产生。结果: 4 μmol/L OA-NO2作用24 h对放射性损伤颌下腺上皮细胞增殖率促进作用最佳;各组细胞培养24 h后,与RG组比较,R+ONG组中Nrf2、HO-1、NQO1蛋白表达明显升高,活性氧的产生明显降低(P<0.05);与R+ONG组比较,R+ON+BRG组中Nrf2、HO-1、NQO1蛋白表达明显降低,活性氧的产生明显升高(P<0.05)。结论: OA-NO2可能经Nrf2/HO-1通路对放射性损伤大鼠颌下腺上皮细胞发挥抗氧化损伤作用。

关键词: 硝基油酸, Nrf2/HO-1通路, 颌下腺上皮细胞, 放射性损伤, 抗氧化损伤

Abstract: Objective: To detect whether nitro-oleic acid (OA-NO2) can exert antioxidant effect on radiation-damaged rat submandibular gland epithelial cells through Nrf2/HO-1 pathway. Methods: The submandibular gland epithelial cells of rats cultured in vitro for 3 days were established by electron linear accelerator, and the CCK-8 method was used to screen the best proliferation concentration of the radial injury cell model OA-NO2 after 24 h. According to the experimental purpose, they were divided into normal cell group (NG), radioactive cell group (RG), radioactive cell + nitro-oleic acid group (R+ONG), radiation cells + nitro-oleic acid + inhibitor group (R+ON+BRG), and radiation cells + inhibitor group (R+BRG). After 24 h of cell culture, the expressions of Nrf2, HO-1, and NQO1 protein were detected by Western blot, the production of reactive oxygen species was detected by reactive oxygen species reagent, and the proliferation rate was detected by CCK-8 method. Results: The CCK-8 results showed that: 4 μmol/L OA-NO2 effect for 24 h promoted the proliferation rate of radial injury submandibular gland epithelial cells best. After 24h of cell culture, in contrast to RG group, the expression of Nrf2, HO-1, and NQO1 proteins in R+ONG group increased significantly, and the production of reactive oxygen species decreased significantly (P<0.05). Compared with R+ONG, the expression of Nrf2, HO-1, and NQO1 protein in R+ON+BRG was significantly reduced and the production of reactive oxygen species was significantly increased (P<0.05). Conclusion: Nitro-oleic acid may exert antioxidant effect on rat submandibular gland epithelial cells after radiation injury through Nrf2/HO-1 pathway.

Key words: nitro-oleic acid, Nrf2/HO-1 signaling pathway, submandibular gland epithelial cells, radiation damage, antioxidant damage

骆勤亮, 张霓霓, 龙远铸, 柏宇, 代敏, 唐建宏, 崔田宁, 黄桂林. 硝基油酸经Nrf2/HO-1通路对放射性损伤大鼠颌下腺上皮细胞的抗氧化损伤作用[J]. 口腔医学研究, 2022, 38(12): 1139-1144.

LUO Qinliang, ZHANG Nini, LONG Yuanzhu, BAI Yu, DAI Min, TANG Jianhong, CUI Tianning, HUANG Guilin. Antioxidant Effects of Nitro-oleic Acid on Radiation-damaged Rat Submandibular Gland Epithelial Cells via Nrf2/HO-1 Pathway[J]. Journal of Oral Science Research, 2022, 38(12): 1139-1144.

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硝基油酸经Nrf2/HO-1通路对放射性损伤大鼠颌下腺上皮细胞的抗氧化损伤作用

Antioxidant Effects of Nitro-oleic Acid on Radiation-damaged Rat Submandibular Gland Epithelial Cells via Nrf2/HO-1 Pathway

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