牙齿移动过程中Piezo1对压力侧牙周组织内IL-1β、IL-6及IL-17的影响

doi:10.13701/j.cnki.kqyxyj.2023.02.009

摘要/Abstract

摘要： 目的: 通过大鼠正畸牙齿移动模型,探究Piezo1在压力侧牙周组织内的表达变化及其对IL-1β、IL-6、L-17表达的影响,进一步了解正畸过程中Piezo1在牙周组织改建中的作用。 方法: 选用60只6~8周龄雄性SD大鼠,随机分为A组(对照组)、B组(加力组)、C组(加力+抑制剂组),另按1、3、5、7、14 d分为5个亚组。B组、C组大鼠构建牙齿移动模型,C组局部注射Piezo1抑制剂。在规定时间点处死大鼠,测量牙齿移动距离,HE染色观察压力侧牙周组织形态学变化,免疫组织化学染色观察压力侧牙周组织内Piezo1及IL-1β、IL-6、IL-17表达情况。 结果: B组和C组的牙齿移动距离随时间而增加,牙周组织形态变化明显,B组的Piezo1及IL-1β、IL-6、IL-17表达均较对照组明显(P<0.05),C组与B组相比,除Piezo1表达无明显差异外(P>0.05),IL-1β、IL-6、IL-17的表达水平在3、5、7 d均较低(P<0.05),但仍高于对照组水平(P<0.05)。 结论: Piezo1参与正畸牙移动过程,影响炎性因子的表达水平,参与正畸牙移动过程中压力侧牙周组织改建。

关键词: 正畸牙移动, Piezo1, 白细胞介素-1β, 白细胞介素-6, 白细胞介素-17

Abstract: Objective: To investigate the distribution of Piezo1 in the pressure side of periodontal tissue and its effect on the expression of IL-1β, IL-6, and IL-17 by using the orthodontic tooth movement model in rats, and to further understand the role of Piezo1 in the reconstruction of periodontal tissue during orthodontic process. Methods: Sixty male SD rats aged 6-8 weeks were randomly divided into group A (control group), group B (force group), and group C (force + inhibitor group), which were further divided into five subgroups according to 1, 3, 5, 7, and 14 days. The teeth movement model was constructed in group B and group C, and Piezo1 inhibitor was locally injected into group C. The rats were sacrificed at the specified time point and the tooth movement distance was measured. HE staining was used to observe the morphological changes of periodontal tissue on the pressure side, and immunohistochemical staining was used to observe the expression of Piezo1, IL-1β, IL-6, and IL-17 in the periodontal tissue on the pressure side. Results: The displacement distances of teeth in group B and group C increased with time, and the morphological changes of periodontal tissue were obvious. The expressions of Piezo1, IL-1β, IL-6, and IL-17 in group B were significantly higher than those in group A (P<0.05). No significant difference was found between group C and group B except Piezo1 (P>0.05). The expression levels of IL-1β, IL-6, and IL-17 were lower at day 3, 5, and 7 (P<0.05), but still higher than those of group A (P<0.05). Conclusion: Piezo1 is involved in the movement of orthodontic teeth, which affects the expression of inflammatory factors and participates in the reconstruction of periodontal tissue of pressure side during orthodontic tooth movement.

Key words: orthodontic tooth movement, Piezo1, interleukins-1β, interleukins-6, interleukins-17

张曼, 张苗苗, 李易, 王贺, 张严匀. 牙齿移动过程中Piezo1对压力侧牙周组织内IL-1β、IL-6及IL-17的影响[J]. 口腔医学研究, 2023, 39(2): 135-140.

ZHANG Man, ZHANG Miaomiao, LI Yi, WANG He, ZHANG Yanyun. Effect of Piezo1 Channel on Expression of IL-1β, IL-6, and IL-17 in Periodontal Tissue of Pressure Side During Tooth Movement[J]. Journal of Oral Science Research, 2023, 39(2): 135-140.

参考文献

[1] Sufarnap E, Siregar D, Lindawati Y. Effect of vitamin E supplementation on orthodontic tooth movement in Wistar rats: a prelimary study [J]. F1000Res, 2020, 9: 1093.
[2] Jeon HH, Teixeira H, Tsai A. Mechanistic insight into orthodontic tooth movement based on animal studies: A critical review [J]. J Clin Med, 2021, 10(8):1733.
[3] Krishnan V, Davidovitch Z. Cellular, molecular, and tissue-level reactions to orthodontic force [J]. Am J Orthod Dentofacial Orthop, 2006, 129(4):469.e1-32.
[4] Jayaprakash PK, Basavanna JM, Grewal H, et al. Elevated levels of Interleukin (IL)-1β, IL-6, tumor necrosis factor-α, epidermal growth factor, and β2-microglobulin levels in gingival crevicular fluid during human Orthodontic tooth movement (OTM) [J]. J Family Med Prim Care, 2019, 8(5): 1602-1606.
[5] Lin T, Yang L, Zheng W, et al. Matrix metalloproteinases and Th17 cytokines in the gingival crevicular fluid during orthodontic tooth movement [J]. Eur J Paediatr Dent, 2021, 22(2):135-138.
[6] Maan AS, Patil AK. Assessment of salivary interleukin-1β (IL-1β), prostaglandin E₂ (PGE₂) levels and pain intensity in children and adults during initial orthodontic treatment [J]. J Orthod Sci, 2019, 8:16.
[7] Dombroski JA, Hope JM, Sarna NS, et al. Channeling the force: Piezo1 mechanotransduction in cancer metastasis [J]. Cells, 2021, 10(11):2815.
[8] Orsini EM, Perelas A, Southern BD, et al. Stretching the function of innate immune cells [J]. Front Immunol, 2021, 12:767319.
[9] Wang L, You X, Lotinun S, et al. Mechanical sensing protein PIEZO1 regulates bone homeostasis via osteoblast-osteoclast crosstalk [J]. Nat Commun, 2020, 11(1):282.
[10] Liu H, Hu J, Zheng Q, et al. Piezo1 channels as force sensors in mechanical force-related chronic inflammation [J]. Front Immunol, 2022, 13:816149.
[11] Jiang Y, Yang X, Jiang J, et al. Structural designs and mechanogating mechanisms of the mechanosensitive piezo channels [J]. Trends Biochem Sci, 2021, 46(6):472-488.
[12] Zhao Q, Zhou H, Li X, et al. The mechanosensitive Piezo1 channel: a three-bladed propeller-like structure and a lever-like mechanogating mechanism [J]. FEBS J, 2019, 286(13):2461-2470.
[13] Bae C, Sachs F, Gottlieb PA. The mechanosensitive ion channel Piezo1 is inhibited by the peptide GsMTx4 [J]. Biochemistry, 2011, 50(29):6295-6300.
[14] Jin Y, Li J, Wang Y, et al. Functional role of mechanosensitive ion channel Piezo1 in human periodontal ligament cells [J]. Angle Orthod, 2015, 85(1):87-94.
[15] Amarasekara DS, Yun H, Kim S, et al. Regulation of osteoclast differentiation by cytokine networks [J]. Immune Netw, 2018, 18(1):e8.
[16] Moon SJ, Ahn IE, Jung H, et al. Temporal differential effects of proinflammatory cytokines on osteoclastogenesis [J]. Int J Mol Med, 2013, 31(4):769-777.
[17] Ruscitti P, Cipriani P, Carubbi F, et al. The role of IL-1β in the bone loss during rheumatic diseases [J]. Mediators Inflamm, 2015, 2015:782382.
[18] Lee KJ, Park YC, Yu HS, et al. Effects of continuous and interrupted orthodontic force on interleukin-1beta and prostaglandin E2 production in gingival crevicular fluid [J]. Am J Orthod Dentofacial Orthop, 2004, 125(2):168-177.
[19] Salla JT, Taddei SR, Queiroz-Junior CM, et al. The effect of IL-1 receptor antagonist on orthodontic tooth movement in mice [J]. Arch Oral Biol, 2012, 57(5):519-524.
[20] Iwasaki LR, Haack JE, Nickel JC, et al. Human interleukin-1 beta and interleukin-1 receptor antagonist secretion and velocity of tooth movement [J]. Arch Oral Biol, 2001, 46(2):185-189.
[21] Jiang C, Li Z, Quan H, et al. Osteoimmunology in orthodontic tooth movement [J]. Oral Dis, 2015, 21(6):694-704.
[22] Feng W, Liu H, Luo T, et al. Combination of IL-6 and sIL-6R differentially regulate varying levels of RANKL-induced osteoclastogenesis through NF-κB, ERK and JNK signaling pathways [J]. Sci Rep, 2017, 7:41411.
[23] Lubberts E. The IL-23-IL-17 axis in inflammatory arthritis [J]. Nat Rev Rheumatol, 2015, 11(7):415-429.
[24] Alvarez C, Suliman S, Almarhoumi R, et al. Regulatory T cell phenotype and anti-osteoclastogenic function in experimental periodontitis [J]. Sci Rep, 2020, 10(1):19018.
[25] Lin T, Yang L, Zheng W, et al. Th17 cytokines and its correlation with receptor activator of nuclear factor kappa B ligand during orthodontic tooth movement [J]. Iran J Immunol, 2020, 17(2):137-143.
[26] Wang Y, Xiao B. The mechanosensitive Piezo1 channel: structural features and molecular bases underlying its ion permeation and mechanotransduction [J]. J Physiol, 2018, 596(6):969-978.
[27] Shinge SAU, Zhang D, Din AU, et al. Emerging Piezo1 signaling in inflammation and atherosclerosis; a potential therapeutic target [J]. Int J Biol Sci, 2022, 18(3):923-941.
[28] Son GY, Shin DM, Hong JH. Bacterial PAMPs and allergens trigger increase in [Ca(2+)]i-induced cytokine expression in human PDL fibroblasts [J]. Korean J Physiol Pharmacol, 2015, 19(3):291-297.