激光活化技术对细菌生物膜的清除效果

doi:10.13701/j.cnki.kqyxyj.2023.07.008

摘要/Abstract

摘要： 目的:评价超声、声波及激光活化技术对细菌生物膜的清除效果。方法:选取健康志愿者龈上及龈下菌斑,羟基磷灰石(hydroxyapatite,HAP)片包被胶原作为载体,厌氧培养21 d形成细菌生物膜,分为对照组和实验组,实验组分别应用传统注射器冲洗(conventional needle irrigation, CNI)、被动超声冲洗(passive ultrasonic irrigation, PUI)、EDDY声波冲洗、Nd: YAG激光、半导体(Diode)激光、Er:YAG激光光子引导光声流(photon-initiated photoacoustic streaming, PIPS)和光波增强光声流(shock wave enhanced emission photoacoustic streaming, SWEEPS)联合1%次氯酸钠(NaOCl)处理,刮取HAP片上细菌生物膜,梯度稀释后厌氧37 ℃恒温培养72 h后进行菌落计数,同时采用荧光定量聚合酶链反应(PCR)方法对细菌通用基因及中间链球菌(Streptococcus intermedius, S. i)基因的拷贝数进行检测。采用SPSS 22.0软件进行统计分析。结果:初始可培养细菌数为(11.33±2.50)×10⁸ CFU/mL,CNI组平均杀菌率最低为71.23%,Nd: YAG及Diode激光平均杀菌率分别为86.46%和87.06%,显著高于CNI组(P<0.01)。PUI、EDDY及PIPS平均杀菌率达98.43%、99.11%及99.34%,均显著高于CNI组、Nd: YAG组及Diode激光组(P<0.01)。SWEEPS杀菌率最高达99.93%,与各组比较差异有统计学意义(P<0.05或P<0.01)。SWEEPS组细菌通用基因及S.i基因拷贝数最少,与对照组、CNI组、Nd: YAG组及Diode激光组比较差异有统计学意义(P<0.05)。结论:激光活化次氯酸钠冲洗液可显著提高对细菌生物膜的灭菌效果,其中Er: YAG激光SWEEPS模式杀菌率最高。

关键词: 细菌生物膜, 根管冲洗, 光波增强光声流, Er:YAG激光光子引导光声流, 激光活化

Abstract: Objective: To evaluate the sterilization effect of laser activated irrigation technique on bacterial biofilm. Methods: Supragingival and subgingival plaque of healthy volunteers were selected. Hydroxyapatite (HAP) disks coated with collagen were used as carrier. After 21 days of anaerobic culture, bacterial biofilms were formed on HAP disks. Specimens were divided into control group and experimental groups, in which experimental groups used conventional needle irrigation (CNI), passive ultrasonic irrigation (PUI), EDDY, Nd:YAG laser, Diode laser, Er:YAG photon-initiated photoacoustic streaming (PIPS), and Er:YAG shock wave enhanced emission photoacoustic streaming (SWEEPS), respectively. After treatment, bacterial biofilms were scraped from HAP disks, diluted by gradient, and cultured at 37 ℃ for 72 h. Meanwhile, quantitative polymerase chain reaction (PCR) was used to detect the copy number of bacterial universal genes and Streptococcus intermedius genes. Results: The total number of bacteria initially culturable by biofilm was (11.33±2.50)×10⁸ CFU/mL. The average sterilization rate of CNI was 71.23%. There was no significant difference in the sterilization rates of Diode and Nd: YAG laser (P>0.05), and average sterilization rates were 86.46% and 87.06%, respectively. PUI, EDDY, and PIPS could significantly improve the sterilization effect of NaOCl compared with CNI (P<0.01), but there was no significant difference between them (P>0.05). The SWEEPS group had the highest sterilization rate of 99.93%, showing significant differences from all groups (P<0.05 or P<0.01). Meanwhile, the SWEEPS group had the lowest number of bacterial universal gene and Streptococcus intermedius genes copies, and there were statistical differences compared to the control group, CNI group, Nd: YAG group, and Diode laser group (P<0.05). Conclusions: Laser activation, especially Er: YAG-SWEEPS mode, could significantly improve the sterilization effect on bacterial biofilm of NaOCl.

Key words: microbial biofilm, root canal irrigation, Er:YAG shock wave enhanced emission photoacoustic streaming, Er:YAG photon-initiated photoacoustic streaming, laser activation

苏征, 杨子, 安婷, 侯本祥. 激光活化技术对细菌生物膜的清除效果[J]. 口腔医学研究, 2023, 39(7): 607-612.

SU Zheng, YANG Zi, AN Ting, HOU Benxiang. Antibiofilm Activity of Irrigation Protocols Activated by Lasers in Vitro[J]. Journal of Oral Science Research, 2023, 39(7): 607-612.

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