3D打印光敏树脂力学性能和细胞毒性的研究

doi:10.13701/j.cnki.kqyxyj.2023.09.013

口腔医学研究 ›› 2023, Vol. 39 ›› Issue (9): 832-837.DOI: 10.13701/j.cnki.kqyxyj.2023.09.013

3D打印光敏树脂力学性能和细胞毒性的研究

张萌萌¹, 陈梓杰¹, 郝鹏杰^2*

1.滨州医学院口腔医学院山东烟台 264003;
2.滨州医学院附属烟台口腔医院修复科山东烟台 264000

收稿日期:2023-02-27 出版日期:2023-09-28 发布日期:2023-09-25
通讯作者: *郝鹏杰,E-mail:1980hpj@163.com
作者简介:张萌萌(1998~ ),女,山东济南人,硕士,研究方向:口腔材料的应用。

Study on Mechanical Properties and Cytotoxicity of 3D Printing Photosensitive Resin

ZHANG Mengmeng¹, CHEN Zijie¹, HAO Pengjie^2*

1. School of Stomatology, Binzhou Medical University, Yantai 264003, China;
2. Department of Prosthodontics, Yantai Stomatological Hospital Affiliated to Binzhou Medical University, Yantai 264000, China

Received:2023-02-27 Online:2023-09-28 Published:2023-09-25

摘要/Abstract

摘要： 目的: 比较3种不同3D打印光敏树脂的力学性能和对人牙龈成纤维细胞(human gingival fibroblasts, HGFs)的细胞毒性,为临床应用提供参考。方法: 选取3种不同的3D打印光敏树脂(WANFAB DC20、T-CRD-141和MED 610)分别制作力学性能测试和细胞毒性测试的标准试件,然后进行弯曲强度、压缩强度、邵氏D硬度和浸提液测试。实验数据采用单因素方差分析和事后多重比较(LSD检验)对组间均值进行对比分析。结果: 3D打印光敏树脂的弯曲强度和压缩强度依次为:MED 610＞WANFAB DC20＞T-CRD-141;邵氏D硬度依次为:WANFAB DC20＞T-CRD-141＞MED 610。WANFAB DC20的细胞毒性高于T-CRD-141和MED 610。结论: WANFAB DC20和T-CRD-141的弯曲强度和压缩强度显著低于MED 610,但邵氏D硬度高于MED 610。WANFAB DC20的细胞毒性高于T-CRD-141,但其力学性能显著优于T-CRD-141,在选择DLP打印材料时优先考虑WANFAB DC20。

关键词: 3D打印光敏树脂, 力学性能, 细胞毒性

Abstract: Objective: To compare the mechanical properties and cytotoxicity of three different 3D printing photosensitive resins on human gingival fibroblasts (HGFs) so as to provide reference for clinical application. Methods: Three different 3D printing photosensitive resins (WANFAB DC20, T-CRD-141, and MED 610) were selected to make the standard specimens for mechanical properties test and cytotoxicity test, and then the flexural strength, compressive strength, Shore D hardness, and extract fluid were tested. The experimental data were compared and analyzed by single factor analysis of variance and multiple comparison afterwards (LSD test). Results: The flexural strength and compression strength of 3D printing photosensitive resin were MED 610>WANFAB DC20>T-CRD-141, and the Shore D hardness was WANFAB DC20>T-CRD-141>MED 610. The cytotoxicity of WANFAB DC20 was higher than that of T-CRD-141 and MED 610. Conclusion: The flexural and compressive strengths of WANFAB DC20 and T-CRD-141 were significantly lower than that of MED 610, but the Shore D hardness was higher than that of MED 610. The cytotoxicity of WANFAB DC20 was higher than that of T-CRD-141, but its mechanical properties were significantly better than those of T-CRD-141. WANFAB DC20 is the preferential selection of DLP printing materials.

Key words: 3D printing photosensitive resin, mechanical properties, cytotoxicity

张萌萌, 陈梓杰, 郝鹏杰. 3D打印光敏树脂力学性能和细胞毒性的研究[J]. 口腔医学研究, 2023, 39(9): 832-837.

ZHANG Mengmeng, CHEN Zijie, HAO Pengjie. Study on Mechanical Properties and Cytotoxicity of 3D Printing Photosensitive Resin[J]. Journal of Oral Science Research, 2023, 39(9): 832-837.

参考文献

[1] Mardis NJ. Emerging technology and applications of 3D printing in the medical field [J]. Mo Med, 2018, 115(4):368-373.
[2] Tian Y, Chen C, Xu X, et al. A review of 3D printing in dentistry: technologies, affecting factors, and applications [J]. Scanning, 2021, 2021:9950131.
[3] Quan H, Zhang T, Xu H, et al. Photo-curing 3D printing technique and its challenges [J]. Bioact Mater, 2020, 5(1): 110-115.
[4] Vermeulen J. The accuracy of implant placement by experienced surgeons: guided vs freehand approach in a simulated plastic model [J]. Int J Oral Maxillofac Implants, 2017, 32(3):617-624.
[5] Flugge TV, Nelson K, Schmelzeisen R, et al. Three-dimensional plotting and printing of an implant drilling guide: simplifying guided implant surgery [J]. J Oral Maxillofac Surg, 2013, 71(8): 1340-1346.
[6] Greenstein G, Cavallaro J, Tarnow D. Practical application of anatomy for the dental implant surgeon [J]. J Periodontol, 2008, 79(10):1833-1846.
[7] Kang SH, Lee JW, Lim SH, et al. Verification of the usability of a navigation method in dental implant surgery: in vitro comparison with the stereolithographic surgical guide template method [J]. J Craniomaxillofac Surg, 2014, 42(7):1530-1535.
[8] Zhou W, Liu Z, Song L, et al. Clinical factors affecting the accuracy of guided implant surgery-a systematic review and meta-analysis [J]. J Evid Based Dent Pract, 2018, 18(1):28-40.
[9] Kessler A, Le V, Folwaczny M. Influence of the tooth position, guided sleeve height, supporting length, manufacturing methods, and resin E-modulus on the in vitro accuracy of surgical implant guides in a free-end situation [J]. Clin Oral Implants Res, 2021, 32(9):1097-1104.
[10] Kessler A, Hickel R, Reymus M. 3D printing in dentistry-state of the art [J]. Oper Dent, 2020, 45(1):30-40.
[11] Shim JS, Kim JE, Jeong SH, et al. Printing accuracy, mechanical properties, surface characteristics, and microbial adhesion of 3D-printed resins with various printing orientations [J]. J Prosthet Dent, 2020, 124(4):468-475.
[12] Unkovskiy A, Bui PH, Schille C, et al. Objects build orientation, positioning, and curing influence dimensional accuracy and flexural properties of stereolithographically printed resin [J]. Dent Mater, 2018, 34(12):e324-e333.
[13] Kozior T, Bochnia J, Gogolewski D, et al. Analysis of metrological quality and mechanical properties of models manufactured with photo-curing polyJet matrix technology for medical applications [J]. Polymers (Basel), 2022, 14(3):408.
[14] Ngan CGY, O'Connell CD, Blanchard R, et al. Optimising the biocompatibility of 3D printed photopolymer constructs in vitro and in vivo [J]. Biomed Mater, 2019, 14(3): 035007.
[15] Liu J, Zhang H, Sun H, et al. The development of filler morphology in dental resin composites: a review [J]. Materials (Basel), 2021, 14(19):5612.
[16] Ilie N, Hilton TJ, Heintze SD, et al. Academy of dental materials guidance-resin composites: part Ⅰ-mechanical properties [J]. Dent Mater, 2017, 33(8):880-894.
[17] Diaz-Arnold AM, Dunne JT, Jones AH. Microhardness of provisional fixed prosthodontic materials [J]. J Prosthet Dent, 1999, 82(5):525-528.
[18] Kim JH, Kwon JS, Park JM, et al. Effects of postpolymerization conditions on the physical properties, cytotoxicity, and dimensional accuracy of a 3D-printed dental restorative material [J]. J Prosthet Dent, 2022:S0022-3913(22)00281-5.
[19] Bayarsaikhan E, Gu H, Hwangbo NK, et al. Influence of different postcuring parameters on mechanical properties and biocompatibility of 3D printed crown and bridge resin for temporary restorations [J]. J Mech Behav Biomed Mater, 2022, 128: 105127.
[20] Hakkinen L, Larjava H, Fournier BP. Distinct phenotype and therapeutic potential of gingival fibroblasts [J]. Cytotherapy, 2014, 16(9): 1171-1186.
[21] Bayarsaikhan E, Lim JH, Shin SH, et al. Effects of postcuring temperature on the mechanical properties and biocompatibility of three-dimensional printed dental resin material [J]. Polymers (Basel), 2021, 13(8):1180.
[22] Bural C, Aktaş E, Deniz G, et al. Effect of post-polymerization heat-treatments on degree of conversion, leaching residual MMA and in vitro cytotoxicity of autopolymerizing acrylic repair resin [J]. Dent Mater, 2011, 27(11):1135-1143.
[23] Xu Y, Xepapadeas AB, Koos B, et al. Effect of post-rinsing time on the mechanical strength and cytotoxicity of a 3D printed orthodontic splint material [J]. Dent Mater, 2021, 37(5):e314-e327.

3D打印光敏树脂力学性能和细胞毒性的研究

Study on Mechanical Properties and Cytotoxicity of 3D Printing Photosensitive Resin

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 11

编辑推荐

Metrics

[1]	段咏华, 梅健, 潘亮, 刘梦石, 郭美玲. 基于生物力学性能分析上颌后牙区骨量不足下短种植体对种植体位移、骨组织应力和应变的影响[J]. 口腔医学研究, 2022, 38(9): 843-847.
[2]	高黎, 王蕊, 张慧, 刘文涛, 马宁. 聚乳酸复合材料及乳前牙根部牙本质的力学性能研究[J]. 口腔医学研究, 2021, 37(2): 139-143.
[3]	刘继涛, 陈庆华. 牙釉质的结构和力学性能研究进展[J]. 口腔医学研究, 2020, 36(3): 213-215.
[4]	罗恒, 高海, 许雪飞, 冯云枝. 杜仲胶复合义齿软衬材料的配方设计和筛选[J]. 口腔医学研究, 2019, 35(4): 386-389.
[5]	郭芳, 黄硕, 朱勇, 薛洋, 胡敏, 高悦榕, 刘昌奎. 3种不同工艺制备聚醚醚酮假体的生物力学性能研究[J]. 口腔医学研究, 2019, 35(4): 405-408.
[6]	史彦, 邓志鹏, 陈浩. 5种根管冲洗液对小鼠成纤维细胞L929的毒性评价[J]. 口腔医学研究, 2018, 34(4): 388-392.
[7]	罗新年,连文伟. 钴离子对人肾小管上皮细胞毒性及Bax、Bcl-2基因表达变化的实验研究[J]. 口腔医学研究, 2016, 32(8): 869-873.
[8]	陈凤英, 程祥荣. 3种义齿粘附剂的体外细胞毒性研究[J]. 口腔医学研究, 2015, 31(5): 457-460.
[9]	刘滋川,李雅静,孙亚杰,谢琪,杜青,谢伟丽. 微纳复合结构钛涂层的制备及其生物相容性研究[J]. 口腔医学研究, 2015, 31(12): 1197-1200.
[10]	詹文辉,江月梅综述,黄玮审校. 烧结对牙科用氧化锆陶瓷性能的影响[J]. 口腔医学研究, 2015, 31(11): 1164-1168.
[11]	曹尤雅,孙宇,程翔,刘桂英,马越,孙英博,王芳. 牙科用锆基非晶合金的细胞毒性及血液相容性研究[J]. 口腔医学研究, 2015, 31(10): 974-977.