SDC4在唾液腺腺样囊性癌神经侵袭中的作用研究

doi:10.13701/j.cnki.kqyxyj.2023.12.011

口腔医学研究 ›› 2023, Vol. 39 ›› Issue (12): 1085-1091.DOI: 10.13701/j.cnki.kqyxyj.2023.12.011

SDC4在唾液腺腺样囊性癌神经侵袭中的作用研究

张艳宁¹, 石宏^2*, 李荷香³, 王洁³, 郝亚丽¹, 王旭⁴, 袁云云¹

1.河北医科大学口腔医学院·口腔医院,河北省口腔医学重点实验室,河北省口腔疾病临床医学研究中心河北石家庄 050017;
2.河北医科大学口腔医学院·口腔医院儿童口腔科河北石家庄 050017;
3.河北医科大学口腔医学院·口腔医院病理科河北石家庄 050017;
4.河北医科大学口腔医学院·口腔医院修复科河北石家庄 050017

收稿日期:2023-06-08 出版日期:2023-12-28 发布日期:2023-12-25
通讯作者: * 石宏,E-mail:shihong_hb@163.com
作者简介:张艳宁(1984~ ),女,河北人,博士,副教授,研究方向:口腔颌面部肿瘤。
基金资助:
河北省自然科学基金(编号:H2017206234)河北省科技厅卫生健康创新专项(编号:22377755D)河北省政府资助省级医学优秀人才项目(编号:361029)

Role of SDC4 in Perineural Invasion of Salivary Adenoid Cystic Carcinoma

ZHANG Yanning¹, SHI Hong^2*, LI Hexiang³, WANG Jie³, HAO Yali¹, WANG Xu⁴, YUAN Yunyun¹

1. Hebei Key Laboratory of Stomatology, Hebei Clinical Research Center for Oral Diseases, School and Hospital of Stomatology, Hebei Medical University, Shijiazhuang 050017, China;
2. Department of Pediatric Dentistry, School and Hospital of Stomatology, Hebei Medical University, Shijiazhuang 050017, China;
3. Department of Oral Pathology, School and Hospital of Stomatology, Hebei Medical University, Shijiazhuang 050017, China;
4. Department of Prosthodontics, School and Hospital of Stomatology, Hebei Medical University, Shijiazhuang 050017, China

Received:2023-06-08 Online:2023-12-28 Published:2023-12-25

摘要/Abstract

摘要： 目的:探讨SDC4在CXCL12介导的唾液腺腺样囊性癌周围神经侵袭中的作用。方法:Transwell趋化试验检测不同浓度CXCL12对SACC-83细胞的趋化性;细胞免疫荧光染色和免疫共沉淀分析SDC4与CXCL12的相互作用;构建靶向敲低SDC4基因的shRNA慢病毒表达载体,敲低SDC4在SACC-83细胞的表达;CCK-8法、细胞划痕和Transwell趋化试验检测细胞增殖、迁移和趋化性;采用裸鼠体内周围神经侵袭模型检测周围神经侵袭能力。结果:CXCL12促进SACC-83细胞迁移,并与其浓度呈正相关(P<0.01); SDC4与CXCL12有相互作用,SDC4基因敲低后,SACC-83细胞在CXCL12诱导下的迁移和趋化性均明显降低(P<0.05),而细胞增殖能力没有明显改变(P>0.05),同时裸鼠体内试验表明,其周围神经侵袭能力明显降低(P<0.05)。结论:SDC4通过与CXCL12相互作用参与腺样囊性癌的周围神经侵袭过程。

关键词: 腺样囊性癌, SDC4, CXCL12, 周围神经侵袭

Abstract: Objective: To investigate the role of SDC4 in the perineural invasion of salivary adenoid cystic carcinoma induced by CXCL12. Methods: Transwell assay was used to evaluate the influence of different concentrations of CXCL12 on the chemotaxis of SACC-83 cells. Immunofluorescence staining and co-immunoprecipitation were performed to validate the interactions between SDC4 and CXCL12. The expression of SDC4 in the SACC-83 cells was silenced by viral transfection by constructing lentivirus-expressing shRNA targeting SDC4. The effect of SDC4 silencing on the proliferation and migration was assessed via the CCK-8, wound healing, and transwell assays. An in vivo perineural invasion model was used to prove the effect of SDC4 silencing on the perineural invasion of the SACC-83 cells. Results: CXCL12 induced the migration of SACC-83 cell in a concentration-dependent manner (P<0.01). Co-immunoprecipitation studies validated these interactions between SDC4 and CXCL12. Furthermore, SDC4 gene expression was silenced by RNAi and verified by real-time polymerase chain reaction and western blotting analysis. The migration and chemotactic efficiency of the SACC-83 cells induced by CXCL12 were significantly decreased via SDC4 silencing (P<0.05). While the proliferation of the SACC-83-SDC4-silenced cells showed no difference compared to that of SACC-83 cells (P>0.05). The perineural invasion ability was significantly inhibited by SDC4 silencing in vivo (P<0.05). Conclusion: SDC4 involved in the perineural invasion of salivary adenoid cystic carcinoma through interaction with CXCL12.

Key words: salivary adenoid cystic carcinoma, SDC4, CXCL12, perineural invasion

张艳宁, 石宏, 李荷香, 王洁, 郝亚丽, 王旭, 袁云云. SDC4在唾液腺腺样囊性癌神经侵袭中的作用研究[J]. 口腔医学研究, 2023, 39(12): 1085-1091.

ZHANG Yanning, SHI Hong, LI Hexiang, WANG Jie, HAO Yali, WANG Xu, YUAN Yunyun. Role of SDC4 in Perineural Invasion of Salivary Adenoid Cystic Carcinoma[J]. Journal of Oral Science Research, 2023, 39(12): 1085-1091.

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SDC4在唾液腺腺样囊性癌神经侵袭中的作用研究

Role of SDC4 in Perineural Invasion of Salivary Adenoid Cystic Carcinoma

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