口腔医学研究

口腔医学研究 ›› 2024, Vol. 40 ›› Issue (2): 156-160.DOI: 10.13701/j.cnki.kqyxyj.2024.02.012

• 口腔生物学研究 • 上一篇    下一篇

白血病抑制因子对牙本质发育的影响

从雅琪, 郭冬花, 祝雨茜, 黄璟, 张宇, 周毅*, 张佳莉*   

  1. 口颌系统重建与再生全国重点实验室,口腔生物医学教育部重点实验室,口腔医学湖北省重点实验室,武汉大学口腔医(学)院 湖北 武汉 430079
  • 收稿日期:2023-07-19 出版日期:2024-02-28 发布日期:2024-02-26
  • 通讯作者: 周毅,E-mail:dryizhou@ whu.edu.cn 张佳莉,E-mail:jiali_zhang@whu.edu.cn
  • 作者简介:从雅琪(1998~ ),女,湖北潜江人,硕士在读,研究方向:牙发育。
  • 基金资助:
    国家自然科学基金(编号:82071090、82001014) 湖北省自然科学基金创新发展联合基金(编号:2022CFD005) 湖北省自然科学基金(编号:2022CFB15)

Influence of LIF on Dentin Development in Mice

CONG Yaqi, GUO Donghua, ZHU Yuxi, HUANG Jing, ZHANG Yu, ZHOU Yi*, ZHANG Jiali*   

  1. State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, China
  • Received:2023-07-19 Online:2024-02-28 Published:2024-02-26

PDF (PC)

36

摘要： 目的:探究白血病抑制因子(leukemia inhibitory factor,LIF)对牙本质发育的影响。方法:免疫组织化学染色观察LIF在出生后2天(post-natal 2,PN2)小鼠成牙本质细胞中的表达。建立小鼠切牙机械损伤模型,统计切牙生长速率;通过微计算机断层扫描技术(micro computed tomography,Micro-CT)、显微硬度计、电子探针测量Lif-/-小鼠及其对照切牙的牙本质密度、硬度及钙磷比(Ca/P);碱性磷酸酶(ALP)染色和茜素红染色观察LIF对成牙本质细胞矿化的影响;免疫荧光染色观察成牙本质细胞Ⅰ型胶原α1(Col1α1)、牙本质基质蛋白1(DMP1)表达情况。结果:LIF随着成牙本质细胞分化程度增加表达含量升高。Lif-/-小鼠切牙新生速率减慢。Lif-/-小鼠切牙近釉牙本质界侧牙本质密度、硬度及Ca/P均降低。ALP及茜素红染色结果显示Lif敲低的成牙本质细胞ALP水平降低、矿化结节数量减少,而加入LIF外源性刺激后可以逆转这一现象。免疫荧光结果显示Lif-/-小鼠成牙本质细胞Col1α1、DMP1表达含量降低。结论:LIF缺失抑制小鼠牙齿牙本质形成与矿化。

关键词: 白血病抑制因子, 牙本质, 矿化, 成牙本质细胞

Abstract: Objective: To investigate the effect of LIF during dentin formation. Methods: Immunohistochemistry (IHC) was employed to detect the expression pattern of LIF during odontoblast differentiation in PN2 incisors. About 2mm from the tip of the erupted part of the left incisor of each mouse was removed to compare different growth speed between Lif-/- group and the control group. The mandibles were scanned by a micro-computed tomography (Micro-CT) instrument. Mimics was used to analyze the mineral density of dentin in both groups. Microhardness measurement was carried out to measure the dentin surface hardness. Electron probe X-ray microanalyser (EPMA) was performed for surface elements analysis of mandibular incisors. Immunofluorescence (IF) was employed to detect the expression of Col1α1 and DMP1 in odontoblasts. Results: LIF was present in polarizing odontoblasts, and gradually increased with the mature of odontoblasts. The growth speed of Lif-/- incisors were slower than that of control group. The mineral density and Vickers hardness of dentin in Lif-/- incisors were lower than that in wild-type mice. The element atomic percent of the Ca/P ratio was reduced in Lif-/- mice. Knockdown of Lif decreased the ALP activity and the formation of mineralized matrix nodules and these could be rescued by adding LIF. The expressions of Col1α1 and DMP1 were decreased in odontoblasts of Lif-/- group. Conclusion: Deficiency of LIF restrains the formation and mineralization of incisor dentin in mice.

Key words: leukemia inhibitory factor, dentin, mineralization, odontoblast