口腔医学研究

口腔医学研究 ›› 2025, Vol. 41 ›› Issue (2): 134-139.DOI: 10.13701/j.cnki.kqyxyj.2025.02.009

• 口腔微生物学研究 • 上一篇    下一篇

负载吲哚菁绿的脂质体纳米颗粒对戈登链球菌的体外抗菌研究

杨青1, 曹士盛2, 郭素蓉1, 穆星彤1, 赵彩玲1, 张娟1*   

  1. 1.天津医科大学口腔医院修复科 天津 300070;
    2.中国医学科学院&北京协和医学院生物医学工程研究所激光医学实验室 天津市 300070
  • 收稿日期:2024-08-02 出版日期:2025-02-28 发布日期:2025-02-26
  • 通讯作者: *张娟,E-mail:Kqzhangjuan@126.com
  • 作者简介:杨青(1998~),女,山东枣庄人,硕士研究生,研究方向:口腔修复学及牙周病学。
  • 基金资助:
    天津市卫生健康委员会科技项目(编号:TJWJ2022MS015)

Antimicrobial Studies of Liposome Nanoparticles Loaded with Indocyanine Green Against Streptococcus Gordonii In Vitro

YANG Qing1, CAO Shisheng2, GUO Surong1, MU Xingtong1, ZHAO Cailing1, ZHANG Juan1*   

  1. 1. Department of Prosthodontics, Tianjin Medical University Stomatological Hospital, Tianjin 300070, China;
    2. Laboratory of Laser Medicine, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300070, China
  • Received:2024-08-02 Online:2025-02-28 Published:2025-02-26

PDF (PC)

37

摘要： 目的: 探讨脂质体装载吲哚菁绿(indocyanine green,ICG)对戈登链球菌的体外光热光动力抗菌性能。方法: 纳米沉淀法将ICG封装在脂质体内得到负载吲哚菁绿的脂质体纳米颗粒(indocyanine green nanoparticles,ICG NPs),并对其粒径及多分散系数进行表征检测,测定ICG NPs的包载率。研究游离ICG、ICG NPs的体外光热性能及活性氧产生情况。通过菌落计数及结晶紫染色评估游离ICG和ICG NPs对戈登链球菌的抗菌效率及其生物膜的影响。利用溶血实验观察ICG NPs的生物相容性。结果: ICG NPs的平均粒径为(146±2) nm,多分散系数为0.263,包封率为93.7%。与对照组相比,当ICG的浓度升高时,其对应的生物膜的生物量和菌落数都会有所降低(P<0.05),ICG NPs则显示出更高的抗菌(99%)、抗生物膜(87%)效率。此外,不同浓度的ICG、ICG NPs经1 W/cm2的近红外光照射后,活性氧(reactive oxygen species,ROS)的数量逐渐增多。ICG NPs的溶血率仅为3.34%。结论: 应用ICG NPs进行抗菌光动力、光热治疗可以有效破坏牙周戈登链球菌及生物膜。

关键词: 脂质体纳米粒, 吲哚菁绿, 光动力疗法, 光热疗法, 戈登链球菌

Abstract: Objective: To examine the antibacterial activities of liposomeloaded with indocyanine green (ICG) against streptococcus gordonii using photothermal photodynamic processes in vitro. Methods: The nanoprecipitation method was used to encapsulate ICG in liposomes to obtain indocyanine green nanoparticles (ICG NPs).The particle size and polydispersity index were characterized, and the encapsulation rate of ICG NPs was measured. The photothermal characteristics and generation of reactive oxygen species(ROS) of free ICG and ICG nanoparticles were studied in vitro. Crystal violet staining and colony counting were used to assess the antibacterial efficacy of free ICG and ICG nanoparticles against streptococcus gordonii and to evaluate their effect on biofilm. A test for hemolysis was used to determine the biocompatibility of ICG nanoparticles. Results: ICG NPs had an encapsulation rate of 93.7%, an average particle size of (146±2) nm, and a polydispersity coefficient of 0.263. Compared to the control group, the number of colonies and biofilm biomass gradually decreased with the increase of ICG concentration under NIR irradiation (P<0.05). Additionally, the ICG NPs exhibited higher antimicrobial (99%) and anti-biofilm (87%) efficiencies. The amount of intracellular ROS gradually increased after different ICG and ICG NPs concentrations were irradiated by 1 W/cm2 of near-infrared light. ICG NPs had a hemolysis rate of only 3.34%. Conclusion: Periodontal streptococcus gordonii and biofilm can be efficiently destroyed by applying ICG NPs for antimicrobial photodynamic and photothermal therapy.

Key words: liposome nanoparticles, ICG, photodynamic therapy, photothermal therapy, streptococcus gordonii