富血小板血浆与富血小板纤维蛋白联合冻干法研究进展

口腔医学研究 ›› 2015, Vol. 31 ›› Issue (10): 1047-1049.

富血小板血浆与富血小板纤维蛋白联合冻干法研究进展

刘丽莎,张斌^*,李莹^*

哈尔滨医科大学附属第二医院,哈尔滨医科大学中俄医学研究中心硬组织发育与再生研究所黑龙江哈尔滨 150086

收稿日期:2014-07-14 出版日期:2015-10-28 发布日期:2016-05-04
通讯作者: 张斌,E-mail:zhangbhyd@126.com;李莹,E-mail:Bonnieli1122@gomail.com
作者简介:刘丽莎(1989~ ),女,辽宁,硕士在读,主要从事口腔科生物材料的研究工作。
基金资助:
国家自然科学基金(编号:81170960)

Research Progress of Freeze-drying and Combined Application of PRP and PRF

LIU Li-sha, ZHANG Bin, LI Yin

Department of Periodontology and Oral Mucosal Membrane, The Affiliated Second Hospital of Harbin Medical University. Harbin 150086, China

Received:2014-07-14 Online:2015-10-28 Published:2016-05-04

摘要/Abstract

摘要： 手术使用的浓缩血小板是再生医学的创新材料,富血小板血浆(PRP)是第一代富含血小板的浓缩物,已广泛应用于促进软硬组织愈合。富血小板纤维蛋白(PRF)是由法国学者Choukroun等首次提出的新一代血小板浓缩物,较传统富血小板血浆更具有应用优势。冻干法是保存生物制品的常规方法,也是控制支架空隙大小并保存其原有成分的有效手段。本文就于富血小板血浆和富血小板纤维蛋白结合冻干法应用于组织发育与再生作一综述。

关键词: 富血小板血浆, 富血小板纤维蛋白, 冻干法

Abstract: Platelet concentrates for surgical use are innovative tools of regenerative medicine. Platelet-rich plasma (PRP) is the first generation of platelet concentrate that has been used widely to accelerate soft-tissue and hard-tissue healing. Platelet-rich fibrin (PRF), a new generation of platelet concentrates, is first described by Choukroun et al.It has been shown to have several advantages over traditional PRP. Freeze-drying is the conventional method to save biological products andis also an effective means to control scaffold pore size and preserve its composition. This article reviewed PRP, PRF, freeze-drying and the combined application in organization development and regeneration.

Key words: Platelet-rich plasma, Platelet-rich fibrin, Freeze-drying

中图分类号:

R318.08

刘丽莎,张斌,李莹. 富血小板血浆与富血小板纤维蛋白联合冻干法研究进展[J]. 口腔医学研究, 2015, 31(10): 1047-1049.

LIU Li-sha, ZHANG Bin, LI Yin. Research Progress of Freeze-drying and Combined Application of PRP and PRF[J]. Journal of Oral Science Research, 2015, 31(10): 1047-1049.

参考文献

[1] Dohan DM, Choukroun J, Diss A, et al. Platelet-rich fibrin (PRF): A second-generation platelet concentrate. Part I: Technological concepts and evolution [J]. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 2006, 101(3)∶e37-44
[2] Gassling VL, Ail Y, Springer IN, et al. Platelet-rich plasma and platelet-rich fibrin in human cell culture [J]. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 2009, 108(1)∶48-55
[3] Ross R, Glomset J, Kariya B, et al. A platelet-dependent serum factor that stimulates the proliferation of arterial smooth muscle cells in vitro [J]. Proc Natl Acad Sci, 1974, 71(4)∶1207-1210
[4] Assoian RK, Grotendorst GR, Miller DM, et al. Cellular transformation by coordinated action of three peptide growth factors from human platelets [J]. Nature, 1984, 309(5971)∶804-806
[5] Choukroun J, Adda F, Schoeffler C, et al. Une opportuniteen paroimplantologie: le PRF [J] Implantodontie, 2000, 42∶55-62.French
[6] Mishra A, Pavelko T. Treatment of chronic elbow tendinosis with buffered platelet-rich plasma [J]. Am. J. Sports Med, 2006, 34(11)∶1774-1778
[7] Mishra A, Woodall J Jr, Vieira A. Treatment of tendon and muscle using platelet-rich plasma [J]. Clin. Sports Med, 2009, 28(1)∶113-125
[8] Kassolis JD, Rosen PS, Reynolds MA. Alveolar ridge and sinus augmentation utilizing platelet rich plasma in combination with freezed-dried bone allograft:case series [J]. J Periodontol, 2000, 71(10)∶1654-1661
[9] Smimon D, Manuel S, Geetha V, et al. Potential for osseous regeneration of platelet-rich plasma--a comparative study in mandibular third molar sockets [J]. Indian Journal of Dental Research, 2004, 15(4)∶133
[10] Pessoa RS, Oliveira SR, Menezes HH, et al. Effects of platelet-rich plasma on healing of alveolar socket: Split-mouth histological and histometric evaluation in Cebus apella monkeys [J]. Indian Journal of Dental Research, 2009, 20(4)∶442-447
[11] Naik B, P Karunakar, M Jayadev,et al. Role of Platelet rich fibrin in wound healing: A critical review [2013]. Journal of conservative dentistry, 2013, 16(4)∶284-293
[12] Dohan Ehrenfest DM, P Doglioli, De Peppo GM, et al. Choukroun's platelet-rich fibrin (PRF) stimulates in vitro proliferation and differentiation of human oral bone mesenchymal stem cell in a dose-dependent way [J]. Archives of Oral Biology, 2010, 55(3)∶185-194
[13] Bonucci E, Marini E, Valdinucci F, et al. Osteogenic response to hydroxyapatite-fibrin implants in maxillofacial bone defects [J]. Eur J Oral Sci, 1997, 105(6)∶557-561
[14] Marx RE, Carlson ER, Eichstaedt RM, et al. Platelet-rich plasma: growth factor enhancement for bone grafts [J]. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 1998, 85(6)∶638-646
[15] Choukroun J, A Diss, A Simonpieri, et al. Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part IV: clinical effects on tissue healing [J]. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology, 2006, 101(3)∶e56-e60
[16] Jang ES, Park JW, Kweon HY, et al. Restoration of peri-implant defects in immediate implant installations by Choukroun platelet-rich fibrin and silk fibroin powder combination graft [J]. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology, 2010,109(6)∶831-836
[17] Keyhan SO, Hemmat S, Badri AA, et al. Use of Platelet-Rich Fibrin and Platelet-Rich Plasma in Comination With Fat Graft: Which Is More Effective During Facial Lipostructure [J]. Journal of Oral and Maxillofacial Surgery, 2013, 71(3)∶610-621
[18] Sclafani, A.P.; Saman, M. Platelet-rich fibrin matrix for facial plastic surgery. Facial. Plast. Surg. Clin. N. Am. 2012,20(2)∶177-186
[19] Sharma A, Pradeep AR. Treatment of 3-wall intrabony defects in patients with chronic periodontitis with autologous Platelet-rich fibrin: A randomized controlled clinical trial [J]. J Periodontol, 2011, 82(12)∶1705-1712
[20] Chang YC, Zhao JH. Effects of Platelet-rich fibrin on human periodontal ligament fibroblasts and application for periodontal infrabony defects [J]. Australian Dental Journal, 2011, 56(4)∶365-371
[21] Zumstein MA, Berger S, Schober M, et al. Leukocyte- and Platelet-rich fibrin (L-PRF) for long-term delivery of growth factor in rotator cuff repair: Review, preliminary results and future directions [J]. Current Pharmaceutical Biotechnology, 2012, 13(7)∶1196-1206
[22] Matsunaga D, Akizuki S, Takizawa T, et al. Compact Platelet-rich fibrin scaffold to improve healing of patellar tendon defects and for medial collateral ligament reconstruction [J]. Knee, 2013, 20(6)∶545-550
[23] Nakajima Y, Kawase T, Kobayashi M, et al. Bioactivity of freeze-dried platelet-rich plasma in an adsorbed form on a biodegradable polymer material [J]. Platelets, 2012, 23(8)∶594-603
[24] Pietramaggiori G, Kaipainen A, Czeczuga JM, et al. Freeze-dried platelet-rich plasma shows beneficial healing properties in chronic wounds [J]. Wound repair and regeneration, 2006, 14(5)∶573-580
[25] Bielanski A, Bergeron H, Lau PC, et al. Microbial contamination of embryos and semen during long term banking in liquid nitrogen [J]. Cryobiology, 2003, 46(2)∶146-152
[26] Morris GJ. The origin, ultrastructure, and microbiology of the sediment accumulating in liquid nitrogen storage vessels [J]. Cryobiology, 2005, 50(3)∶231-238
[27] Li Q, Reed DA, Min L, et al. Lyophilized Platelet-Rich Fibrin (PRF) Promotes Craniofacial Bone Regeneration through Runx2 [J]. International journal of molecular sciences, 2014, 15(5)∶8509-8525