口腔医学研究

口腔医学研究 ›› 2019, Vol. 35 ›› Issue (12): 1173-1176.DOI: 10.13701/j.cnki.kqyxyj.2019.12.018

• 牙周病学研究 • 上一篇    下一篇

牙周再生术后2周和12周牙周组织对正畸力应答的实验研究

马冲1, 于寰2, 康健2, 张源2, 田静2, 李新月2*   

  1. 1. 南开大学医学院 天津 300041;
    2. 天津市口腔医院 天津 300041
  • 收稿日期:2019-03-22 出版日期:2019-12-28 发布日期:2019-12-23
  • 通讯作者: 李新月,E-mail:lisaxinyue1215@126.com
  • 作者简介:马冲(1990~ ),男,安徽人,医师,硕士在读,研究方向:牙周正畸联合治疗。

Experimental Study on Orthodontic Force Response in Periodontal Tissue after Periodontal Regeneration

MA Chong1, YU Huan2, KANG Jian2, ZHANG Yuan2, TIAN Jing2, LI Xinyue2*   

  1. 1. Medical College, Nankai University. Tianjing 300041, China;
    2. Stomatology Hospital of Tianjing. Tianjing 300041, China.
  • Received:2019-03-22 Online:2019-12-28 Published:2019-12-23

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摘要： 目的:研究不同正畸开展时机对牙齿移动速度及牙周组织再生的影响,以评价牙周组织对正畸力的应答反应。方法:建立巴马小型猪牙周骨缺损模型,应用富血小板纤维蛋白联合牙周植骨术治疗骨缺损,分别在再生后2周和12周开展正畸牵引,正畸6周后处死动物,行牙周指标、Micro CT、组织形态学及免疫组织化学检测。结果:与再生后12周正畸组相比,牙周再生后2周正畸组牙齿移动速度更快(P=0.025);骨量正相关指标包括相对骨体积、骨小梁数量、骨小梁厚度均较高(P=0.019、0.014、0.024);而骨量负相关指标:骨小梁分离度、骨表面积和骨体积的比值、骨小梁模式因子均较低(P=0.033、0.029、0.008);正畸骨改建更为活跃,新生骨量更多;骨保护蛋白和骨形成蛋白2表达水平均更高(P=0.006、0.015)。结论:牙周再生术后2周较12周正畸牙齿移动速度更快,新骨生成更多,骨保护蛋白和成骨蛋白表达更高。

关键词: 植骨术, 富血小板纤维蛋白, 正畸, Micro-CT, 免疫组化

Abstract: Objective: To study the effects of orthodontic timing (2 and 12 weeks) on tooth movement speed and periodontal tissue regeneration, and to evaluate the response of periodontal tissue to orthodontic force. Methods: A periodontal bone defect model in Bama miniature pigs was established. Platelet-rich fibrin (PRF) combined with periodontal bone grafting was used to treat bone defect. Orthodontic traction was carried out at 2 and 12 weeks after regeneration. Animals were sacrificed after 6 weeks of orthodontics. Periodontal parameters, micro-CT, histomorphology, and immunohistochemistry were examined. Results: Compared with 12 weeks after regeneration, the orthodontic group at 2 weeks after regeneration had a faster tooth movement speed (P=0.025). Positive correlations of bone mass included higher relative bone volume, number of bone trabecular and thickness of bone trabecular (P=0.019, P=0.014, P=0.024). Negative correlations of bone mass: trabecular segregation, ratio of bone surface area to bone volume, and trabecular mode factor were lower. (P=0.033, P=0.029, P=0.008). Orthodontic bone remodeling was more active, new bone mass was more, and the expression levels of osteoprotegerin and bone morphogenetic protein 2 were higher (P=0.006, P=0.015). Conclusion: Two weeks after periodontal regeneration, the orthodontic teeth move faster, new bone formation is more, and the expressions of osteoprotegerin and osteogenic protein are higher.

Key words: bone grafting, platelet-rich fibrin, orthodontic, Micro-CT, immunohistochemistry