口腔医学研究

口腔医学研究 ›› 2015, Vol. 31 ›› Issue (2): 113-116.

• 基础研究论著 • 上一篇    下一篇

牙周膜干细胞在磷酸三钙/壳聚糖支架上生长的体外实验

钟志华1,施斌2,周先略1*   

  1. 1. 荆州市中心医院口腔科 湖北 荆州 434000;
    2. 武汉大学口腔医院种植科
  • 收稿日期:2014-06-04 出版日期:2015-02-28 发布日期:2016-04-29
  • 通讯作者: 周先略,电话:0716-8498327
  • 作者简介:钟志华(1977~ ),男,湖北天门人,博士,副主任医师,主要研究方向牙周膜干细胞。
  • 基金资助:
    湖北省卫生厅资助项目(2009-JX4B62)

Experiment of PDLSC Cultured on TCP/Chitosan Scaffold in Vitro.

ZHONG Zhi-hua, SHI Bin, ZHOU Xian-lue, et al   

  1. Department of Stomatology, Jingzhou Central Hospital, Jingzhou 434000
  • Received:2014-06-04 Online:2015-02-28 Published:2016-04-29

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摘要： 目的:检测磷酸三钙(TCP)/壳聚糖支架是否适合培养牙周膜干细胞(PDLSC)。方法:制作3种不同比例TCP/壳聚糖支架,检测支架生物相容性,加入PDLSC和TCP/壳聚糖支架混合培养,扫描电镜检测和碱性磷酸酶(ALP)活性检测。结果:扫描电镜检测到PDLSC在TCP/壳聚糖支架上生长良好,TCP/壳聚糖支架比单纯壳聚糖支架培养PDLSC的ALP活性高。结论:TCP/壳聚糖支架比单纯壳聚糖支架更能促进PDLSC分化。

关键词: 牙周膜干细胞, 磷酸三钙, 壳聚糖, 支架

Abstract: Objective: To test TCP/Chitosan scaffold that PDLSC cultuled on it. Methods: We make three kinds of TCP/Chitosan scaffolds, then test the biocompatibility of scaffolds. After that, we use PDLSC cultured on TCP/Chitosan scaffold, then, do SEM test, and measure ALP activity. Results: PDLSC grow well on TCP/Chitosan scaffold by SEM test.TCP/Chitosan scaffold is higher than pure Chitosan scaffold on ALP activity test of culturing PDLSC.Conclusion:TCP/Chitosan scaffold is better than pure Chitosan scaffold on promoting PDLSC differentiation.

Key words: PDLSC , TCP, Chitosan, Scaffold

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