血竭素高氯酸盐对舌鳞癌细胞Tca-8113增殖及凋亡的影响

口腔医学研究 ›› 2015, Vol. 31 ›› Issue (3): 254-257.

血竭素高氯酸盐对舌鳞癌细胞Tca-8113增殖及凋亡的影响

周彩然¹, 郭淑娟², 曾锦², 黄海森², 姜思聪², 田卫东^2,3*, 邱嘉旋^1*

1. 南昌大学第一附属医院口腔颌面外科江西南昌 330006;
2. 四川大学口腔再生医学国家地方联合工程实验室;
3. 四川大学华西口腔医院口腔颌面外科

收稿日期:2014-08-04 出版日期:2015-03-28 发布日期:2016-04-29
通讯作者: 田卫东,E-mail:drtwd@sina.com
作者简介:周彩然(1987~ ),女,山东临沂人,硕士,主要从事口腔临床工作。
基金资助:
江西省自然科学基金项目(编号:20114BAB205055)

Effect of Dracorhodin Perchlorate on the Proliferation and Apoptosis of Tongue Squamous Carcinoma Cell Line Tca-8113.

ZHOU Cai-ran, GUO Shu-juan, ZENG Jin, et al.

Department of Oral and Maxillofacial Surgery, First Affiliated Hospital of Nanchang University, Nanchang 330006

Received:2014-08-04 Online:2015-03-28 Published:2016-04-29

摘要/Abstract

摘要： 目的:研究血竭素高氯酸盐(Dracorhodin perchlorate,DP)对舌鳞癌细胞Tca-8113增殖和凋亡的影响并初步探讨其相关机制。方法:以舌鳞癌细胞Tca-8113为研究对象,采用CCK-8、流式细胞术分析及Western Blot等方法检测不同浓度DP对Tca-8113细胞的增殖、凋亡及核因子E2-相关因子2(nuclear factor E2-related factor 2,Nrf2)、血红素氧合酶 1(heme oxygenase 1,HO-1)蛋白表达的变化。采用SPSS17.0统计软件对实验结果进行分析。结果:CCK-8检测表明,与空白对照组相比,DP可以显著抑制细胞增殖,且对细胞的增殖抑制作用有时间-剂量依赖性(P<0.05)。流式细胞术及Western Blot检测结果显示DP可以促进细胞凋亡(P<0.05),并提高Nrf2、HO-1在Tca-8113细胞中的表达。结论:DP能抑制舌鳞癌细胞Tca-8113增殖并诱导其发生凋亡,其作用可能与Nrf2/HO-1信号通路激活有关;可能成为抗口腔癌药物。

关键词: 血竭素高氯酸盐, Nrf2/HO-1, Tca-8113, 凋亡

Abstract: Objective: To investigate the effect of dracorhodin perchlorate (DP) on the cell proliferation and apoptosis and to discuss relevant signaling pathways in human tongue squamous carcinoma cell line Tca-8113 . Methods: The Tca8113 cells were used as research object in this experiment. The growth, apoptosis and the expression of nuclear factor E2-related factor 2 (Nrf2) and heme oxygenase 1(HO-1) were detected in human tongue carcinoma cell line(Tca-8113), using cell counting kit-8 (CCK-8), the flow cytometry and Western blot to assay. All statistical analysis was performed using the SPSS 17.0 statistical package. Results: CCK-8 assay proved that there was a much pronounced decrease in cell viability on treatment with DP compared with control group and the time- and dose-dependent effects of treatment with DP on the growth of cells (P<0.05). Flow cytometric analysis and Western blot demonstrated that DP promoted cell apoptosis (P<0.05) and elevated the expression of Nrf2 and HO-1 proteins in Tca-8113 cells. Conclusion: DP inhibited tongue squamous carcinoma Tca-8113 cells growth and induced apoptosis, which may be relevant with the activation of Nrf2/HO-1signaling pathway in oral cancer cells. DP could be developed as an agent against OSCC.

Key words: Dracorhodin perchlorate , Nrf2/HO-1 , Tca-8113, Apoptosis

中图分类号:

R780.1

周彩然, 郭淑娟, 曾锦, 黄海森, 姜思聪, 田卫东, 邱嘉旋. 血竭素高氯酸盐对舌鳞癌细胞Tca-8113增殖及凋亡的影响[J]. 口腔医学研究, 2015, 31(3): 254-257.

ZHOU Cai-ran, GUO Shu-juan, ZENG Jin, et al.. Effect of Dracorhodin Perchlorate on the Proliferation and Apoptosis of Tongue Squamous Carcinoma Cell Line Tca-8113.[J]. Journal of Oral Science Research, 2015, 31(3): 254-257.

参考文献

[1] Podlodowska J, Szumi P J, Podlodowski W, et al. Epidemiology and risk factors of the oral carcinoma [J]. Pol Merkur Lek, 2012, 32(188)∶135-137
[2] Petersen P E. Oral cancer prevention and control-the approach of the World Health Organization [J]. Oral Oncol, 2009, 45(4)∶454-460
[3] Cragg G M, Newman D J. Natural products: A continuing source of novel drug leads [J]. BBA -Gen Subjects, 2013, 1830(6)∶3670-3695
[4] Newman D J, Cragg G M. Natural Products as Sources of New Drugs over the Last 25 Years [J]. J Nat Prod, 2007, 70(3)∶461-477
[5] Gupta D, Bleakley B, Gupta R K. Dragon's blood: Botany, chemistry and therapeutic uses [J]. J Ethnopharmaco, 2008, 115(3)∶361-380
[6] Wei W, Di J, Rasul A, et al. Dracorhodin perchlorate induces apoptosis in bladder cancer cells through Bcl-2, Bcl-XL, survivin down-regulation and caspase-3 activation [J]. Bangladesh J Pharmacol, 2013, 8(3)∶276-282
[7] Tsai J R, Wang H M, Liu P L, et al. High expression of heme oxygenase-1 is associated with tumor invasiveness and poor clinical outcome in non-small cell lung cancer patients [J]. Cell Oncol, 2012, 35(6)∶461-471
[8] Berberat P O, Dambrauskas Z, Gulbinas A, et al. Inhibition of heme oxygenase-1 increases responsiveness of pancreatic cancer cells to anticancer treatment [J]. Clin Cancer Res, 2005, 11(10)∶3790-3798
[9] Duckles H, Boycott H E, Al-Owais M M, et al. Heme oxygenase-1 regulates cell proliferation via carbon monoxide-mediated inhibition of T-type Ca2+ channels [J]. Pflüg Arch Eur J Phy, 2014∶1-13
[10] Alam J, Stewart D, Touchard C, et al. Nrf2, a Cap'n'Collar transcription factor, regulates induction of the heme oxygenase-1 gene [J]. J Biol Chem, 1999, 274(37)∶26071-26078
[11] Lee YM, Jeong JS, DaeLim H, et al. Isoliquiritigenin 2-methyl ether induces growth in hibition and apoptosis in oral cancer cells via heme oxygenase-1 [J]. Toxicol in Vitro, 2010, 24(3)∶776-782
[12] Zhang X, Su Y, Zhang M, et al. Opposite effects of arsenic trioxide on the Nrf2 pathway in oral squamous cell carcinoma in vitro and in vivo [J]. Cancer lett, 2012, 318(1)∶93-98
[13] Lee YM, Auh QS, Lee DW, et al. Involvement of Nrf2-Mediated Upregulation of Heme Oxygenase-1in Mollugin-Induced Growth Inhibition and Apoptosis in Human Oral Cancer Cells [J]. Biomed Res Int, 2013, 1-14

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