口腔医学研究

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (5): 440-444.DOI: 10.13701/j.cnki.kqyxyj.2016.05.004

• 基础研究论著 • 上一篇    下一篇

以牛心包为来源的天然衍生引导骨组织再生膜制备方法的实验研究

白明海1* ,张婷婷2 ,谢辉3 ,吴汉江4 ,赵志河5 ,何正权1 ,刘兴玉   

  1. 1. 长沙市口腔医院正畸科 湖南 长沙 410005;
    2. 中南大学湘雅二医院检验科 湖南 长沙 410011;
    3. 长沙市口腔医院牙周科 湖南 长沙 410005;
    4. 中南大学湘雅二医院口腔科 湖南 长沙 41001;
    5. 四川大学华西口腔医院 四川 成都 610041
  • 收稿日期:2016-01-11 出版日期:2016-05-26 发布日期:2016-05-26
  • 通讯作者: 白明海,电话:13574854875
  • 作者简介:白明海(1977~ ),男,河北人,博士后,副主任医师,主要研究方向:口腔正畸低摩擦力高效矫治及相关基础研究,口腔医学生物材料。
  • 基金资助:
    长沙市科技局项目(编号:0803160-32)

Preparation of Naturally Derived Acellular Bovine Pericardium for Guiding Bone Regeneration.

BAI Ming-hai1*, ZHANG Ting-ting2, XIE Hui3, WU Han-jiang4, ZHAO Zhi-he5, HE Zheng-quan1, LIU Xin-yu1.   

  1. 1. Department of Orthodontics, Changsha Stomatology Hospital, Changsha 410005, China;
    2. Department of Laboratory, Xiangya Second Hospital. Central South University, Changsha 410011, China;
    3.Department of Periodontology,ChangshaStomatology Hospital, Changsha 410005,China;
    4. Department of Stomatology, Xiangya Second Hospital, Central South University, Changsha 410011, China;
    5. West China College of Stomatology, Sichuan University, Chengdu 610041, China.
  • Received:2016-01-11 Online:2016-05-26 Published:2016-05-26

PDF (PC)

334

被引次数

1

摘要： 目的:探索天然牛心包脱细胞和交联处理的理想方法。方法:用胰酶去垢剂法(I)、胰酶核酸酶法(II)、胰酶去垢剂+核酸酶联合法(III)、 冻融去污剂法(IV)、冻融核酸酶法(V)、冻融去污剂+核酸酶联合法(VI)进行脱细胞和交联处理,采用光镜和SEM、细胞毒性实验、机械性能测定、细胞趋化性实验评估处理效果,筛选出理想的脱细胞和交联方法。结果:冻融去污剂+核酸酶联合法是理想的脱细胞方法。京尼平交联法是理想的交联方法。结论:冻融去污剂+核酸酶联合法制备的脱细胞牛心包再采用京尼平交联法可制备出理想的GBR膜材料。

关键词: 天然衍生牛心包脱细胞处理交联处理, GBR膜材料

Abstract: Objective: To find out the best way for decellularization and cross-linking of naturally derived bovine pericardiumto prepare GBR membrane which can meet the clinical demand. Methods: Natural bovine pericardium were decellularized using trypsin+ detergent method (I), trypsin +enzymatic nucleic acid method (II), trypsin+ detergent +enzymatic nucleic acid method (III), freeze- thaw+detergent method (IV), freeze-thaw + enzymatic nucleic acid method (V), or freeze- thaw+detergent + enzymatic nucleic acid method (VI), and then were cross-linked using glutaraldehyde or genipin. The best way of decellularization and cross-linked method were screened by means of optical microscope, scanning electron microscope, cell toxicity test, determination of the mechanical properties, and measurement of the chemotactic properties. Results: Freeze- thaw+detergent + enzymatic nucleic acid method with bovine pericardium was the best method of decellularization. Genipin crosslinking method by which acellular bovine pericardium was crosslinked was better. Conclusion: Freeze- thaw+detergent + enzymatic nucleic acid method and genipin crosslinking method were ideal method for preparation of natural bovine pericardium.

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