小鼠牙髓干细胞同种异体移植后体内免疫反应初探

doi:10.13701/j.cnki.kqyxyj.2017.01.003

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (1): 10-14.DOI: 10.13701/j.cnki.kqyxyj.2017.01.003

小鼠牙髓干细胞同种异体移植后体内免疫反应初探

张苗,李伯琦,郑丽珠,刘奕杉^*

新疆医科大学第一附属医院儿童口腔科--口腔预防科新疆乌鲁木齐 830054

收稿日期:2016-07-20 出版日期:2017-01-25 发布日期:2017-01-22
通讯作者: 刘奕杉,E-mail:lys-tree@126.com
作者简介:张苗(1991~ ),女,新疆人,硕士在读,主要从事儿童口腔医学及乳牙牙髓干细胞方面的研究。
基金资助:
国家自然科学基金(编号:81560178)

Study on Mice’S Immune Response in Vivo after Dental Pulp Stem Cells Allogeneic Transplantation.

ZHANG Miao, LI Bo-qi, ZHENG Li-zhu, LIU Yi-shan^*.

Department of Pediatric Dentistry and Preventive Dentistry, The First Affiliated Hospital, Xinjiang Medical University. Urumqi 830054, China

Received:2016-07-20 Online:2017-01-25 Published:2017-01-22

摘要/Abstract

摘要： 目的:初步探究小鼠牙髓干细胞同种异体移植后的全身免疫反应。方法:牙髓干细胞分离获得自C57BL/6 小鼠牙髓组织,培养至第3 代,与纳米羟基磷灰石(n-HA)复合培养形成复合物,扫描电镜观察其生长黏附情况。40只4周龄的C57BL/6 小鼠随机分成两组。实验组将复合物移植入上背部皮下,对照组将纳米羟基磷灰石植入上背部皮下,术后均使用外科方法严密缝合创口。两组动物术后均观察4周、6周处死,分离脾脏,制成脾淋巴细胞悬液,流式细胞仪测定CD4⁺、CD8⁺的值。结果:牙髓干细胞在体外与纳米羟基磷灰石结合良好;免疫组化染色实验组4周、6周均可见牙本质涎蛋白(dentin sialoprotein,DSP)阳性表达;实验组与对照组相比,术后4周、6周CD4⁺/CD8⁺值均无明显差异。结论:牙髓干细胞同种异体移植后,尚不能够引起机体的免疫排斥反应,其在体内仍具有低免疫原性,有望成为组织工程同种异体种子细胞来源。

关键词: 牙髓干细胞, 同种异体, 移植, 体内, 低免疫原性

Abstract: Objective: To explore the systemic immune response of mice after dental pulp stem cells (DPSCs) allogeneic transplantation. Methods: Dental pulp stem cells were isolated from C57BL/6 mice’s pulp, cultured to the third generation in vitro, and then cultured together with the nanometer hydroxyapatite into a composite. Scanning electron microscope was used to observe the growth condition of the composites. Forty C57BL/6 mice of four weeks were randomly divided into experimental group and control group. The experimental group mice were transplanted the composite under the skin of upper back. And the control group mice were transplanted nanometer hydroxyapatite under the skin of upper back. Two groups of animals were observed 4 and 6 weeks, respectively. The splenic cells of mice were prepared by a stainless steel mesh. Flow cytometry instrument was used to measure the value of CD4⁺ and CD8⁺. Results: Dental pulp stem cells were well bonded with nanometer hydroxyapatite in vitro. Immunohistochemical study showed that both 4 weeks and 6 weeks experimental groups had positive expressions of the dentin sialoprotein (DSP). After 4 and 6 weeks, the values of CD4⁺ and CD8⁺ had no obvious differences between the experimental group and the control group. Conclusion: The results of our experiments proved that after allogeneic transplantation of dental pulp stem cells, it is impossible to cause the body's immune rejection. Dental pulp stem cells still have low immunogenicity in vivo, which are expected to be a source of allograft tissue engineering seed cells in the future.

Key words: Dental, pulp, stem, cell, Allogenic, Transplant, In, vivo, Low, immunogenicity

中图分类号:

R780.1

张苗,李伯琦,郑丽珠,刘奕杉. 小鼠牙髓干细胞同种异体移植后体内免疫反应初探[J]. 口腔医学研究, 2017, 33(1): 10-14.

ZHANG Miao, LI Bo-qi, ZHENG Li-zhu, LIU Yi-shan.. Study on Mice’S Immune Response in Vivo after Dental Pulp Stem Cells Allogeneic Transplantation.[J]. Journal of Oral Science Research, 2017, 33(1): 10-14.

参考文献

[1] Gronthos S, Mankani M, Brahim J, et al. Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo [J]. Proc Natl Acad Sci U S A, 2000, 97(25)∶13625- 13630
[2] Coppe C, Zhang Y, Den Besten PK. Characterization of primary dental pulp cells in vitro [J]. Pediatr Dent,2009,31(7))∶467-471
[3] 杨雪超.CD146在人牙髓干细胞中表达的研究[J].口腔医学研究,2016,32(2))∶122-125
[4] Ding G, Niu J, Liu Y. Dental pulp stem cells suppress the proliferation of lymphocytes via transforming growth factor-β1 [J]. Hum Cell,2011,28(2))∶81-90
[5] Tang R,Ding G. Swine dental pulp stem cells inhibit T-cell proliferation [J]. Transplant Proc,2011,43(10))∶3955-3959
[6] Karamzadeh R, Eslaminejad MB, Aflatoonian R. Isolation, characterization and comparative differentiation of human dental pulp stem cells derived from permanent teeth by using two different method [J]. Vis Exp,2012 ,(69))∶4372
[7] Yang X, Zhang W, van den Dolder J, et al. Multilineage potential of STRO-1+ rat dental pulp cells in vitro [J]. Tissue Eng Regen Med,2007,1(2))∶128-35
[8] 王冉旭,潘爽,何丽娜,等.人牙髓干细胞在不同HA含量的pLGA/HA复合支架材料上的粘附研究[J].口腔医学研究,2015,31(12)∶1171-1173
[9] Masataka Y, Norimasa T, Tadao T, et al. Osteogenic effect of hyaluronic acid sodium salt in the pores of a hydroxyapatite scaffold [J]. Materials Sci Engin, 2007, 27(2))∶220-226
[10] 马路平,钟良军,张源明,等.小型猪牙周膜干细胞在羟基磷灰石支架材料上的生长[J].中国组织工程研究,2013,(29))∶5290-5295
[11] Niu LL, Zheng M, Cao F, et al. Migration and differentiation of exogenous rat mesenchymal stem cells engrafted into normal and injured hearts of rats [J]. Zhonghua Yi Xue Za Zhi, 2004,84(1))∶38-42
[12] 何维.医学免疫学[M].第2版.北京:人民卫生出版社,2010)∶22
[13] Harada H, Kettunen P, Jung HS, et al.Localization of putative stem cells in dental epithelium and their association with Notch and FGF signaling [J]. Cell Biol, 1999 ,147(1))∶105-120
[14] Deans RJ, Moseley AB. Mesenchymal stem cells: biology and potential clinical uses [J]. Exp Hematol, 2000, 28(8))∶875-884
[15] 聂姗姗,刘佳,张瑞涵,等.牙髓干细胞MHC分子表达与体外混合淋巴细胞的增殖[J].中国组织工程研究,2014,(50))∶8162-8167
[16] Fonteneau JF, Larsson M, Somersan S, et a1.Generation of high quantities of viral and tumor-specific human CD4+ and CD8+ T-cell clones using peptide pulsed mature dendritic cells [J]. Immunol Methods, 2001, 258(1-2)∶111-126
[17] Zhu Q, Gibson MP, Liu Q, et al. Proteolytic processing of dentin sialophospho- -protein (DSPP) is essential to dentinogenesis [J]. Biol Chem,2012,287(36))∶30426-30435
[18] 铁晓敏,刘佳,王丽萍,等.牙髓干细胞修复再生同质异体大鼠牙髓牙本质复合体的研究[J].口腔医学研究,2015,31(7)∶645-648

小鼠牙髓干细胞同种异体移植后体内免疫反应初探

Study on Mice’S Immune Response in Vivo after Dental Pulp Stem Cells Allogeneic Transplantation.

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