口腔医学研究

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (3): 269-272.DOI: 10.13701/j.cnki.kqyxyj.2017.03.009

• 临床研究论著 • 上一篇    下一篇

1,25(OH)2D3对2型糖尿病伴牙周炎患者中性粒细胞凋亡及炎性介质表达的影响

闫艳梅1,2，唐亚平1，贺涛3，马灿灿4，方慧1，钟雯怡1，刘琪1   

  1. 1. 遵义医学院附属口腔医院 贵州 遵义 563003;
    2. 岳阳市一人民医院口腔科 湖南 岳阳 414000;
    3. 长沙市口腔医院 湖南 长沙 410005;
    4. 邹城市口腔医院 山东 邹城 273500
  • 收稿日期:2016-08-23 出版日期:2017-03-20 发布日期:2017-03-22
  • 通讯作者: 刘琪,E-mail: liuqi1964@hotmail.com
  • 作者简介:闫艳梅(1989~ ),女,山东菏泽人,医师,硕士,主要从事口腔内科临床工作。
  • 基金资助:
    国家自然基金(编号:81360168)贵州省科学技术基金,黔科合字(2014)7596号遵义市科技支撑计划项目,遵市科合社字(2013)31号

Effects of 1,25-dihydroxyvitamin D3 on Neutrophil Apoptosis and Inflammatory Mediators in Type 2 Diabetic Patients with Chronic Periodontitis.

YAN Yan-mei1,2, TANG Ya-ping1, HE Tao3, MA Can-can4, FANG Hui1, ZHONG Wen-yi1, LIU Qi1.   

  1. 1. Affiliated Stomatological Hospital of Zunyi Medical College, Zunyi 563003, China;
    2. Department of Stomatology, First People's Hospital of Yueyang, Yueyang 414000, China;
    3.Changsha Stomatological Hospital Changsha 410005, China;
    4. Zoucheng Stomatological Hospital Zoucheng 273500, China.
  • Received:2016-08-23 Online:2017-03-20 Published:2017-03-22

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摘要： 目的:探讨1,25(OH)2D3对2型糖尿病伴牙周炎患者中性粒细胞(Polymorphonuclear leukocytes,PMN)凋亡及炎性介质表达的影响。方法:采用密度梯度离心法分离2型糖尿病伴牙周炎患者外周血PMN,然后于含或不含1,25(OH)2D3培养液中培养24 h,分别运用流式细胞术(FCM)检测PMN凋亡率;酶联免疫吸附试验(ELISA)检测上清液中肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)、中性粒细胞弹性蛋白酶(neutrophil elastase,NE)的含量变化。结果:与1,25(OH)2D3未干预组相比,1,25(OH)2D3干预组PMN凋亡率均升高,P<0.05; 1,25(OH)2D3干预组两两比较发现,10-8 mol/L干预组PMN凋亡率＞10-6 mol/L干预组＞10-10 mol/L干预组(P<0.05)。ELISA检测PMN上清液中肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)、中性粒细胞弹性蛋白酶(NE)的结果显示,1,25(OH)2D3干预组TNF-α、IL-1β、IL-6、NE的水平均较未干预组低(P<0.05)。结论:1,25(OH)2D3能够促进PMN凋亡,下调炎性介质的表达,在2型糖尿病伴牙周炎的发生、发展中可能起免疫调节作用。

关键词: 1,25(OH)2D3, 2型糖尿病伴牙周炎, 中性粒细胞, 凋亡

Abstract: Objective: To investigate the effect of 1,25 (OH)2D3 on neutrophils apoptosis and inflammatory mediators expression in Type 2 diabetic patients with chronic periodontitis. Methods: Neutrophils were isolated by density gradient centrifugation from Type 2 diabetic patients with chronic periodontitis peripheral blood and cultured in media with or without 1,25 (OH)2D3 for 24h. The apoptosis rate of neutrophils was detected by flow cytometry (FCM). Expressions of tumor necrosis factor-α, interleukin-1β, interleukin-6, and neutrophil elastase in supernatants were tested by enzyme-linked immunosorbent assay (ELISA). Results: Flow cytometry analysis showed that 1,25(OH)2D3 intervention group neutrophil apoptosis rate was increased significantly (P<0.05). Multiple comparison revealed that there were significant differences among groups with different concentrations (10-8 mol/L intervention group>10-6 mol/L intervention group>10-10mol/L intervention group). ELISA results showed thattumor necrosis factor-α, interleukin -1β, and interleukin-6 content were decreased in 1,25(OH)2D3 intervention group (P<0.05). Conclusion: 1,25(OH)2D3 may promote the neutrophil apoptosis and decrease the expression of inflammatory mediators, and play an immune regulatory role in the occurrence and development in Type 2 diabetic patients with chronic periodontitis.

Key words: 1,25-dihydroxyvitamin , D3 Type 2 diabetes, Periodontitis, Neutrophils, Apoptosis

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