口腔医学研究

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (5): 482-485.DOI: 10.13701/j.cnki.kqyxyj.2017.05.005

• 基础研究论著 • 上一篇    下一篇

IL-24可能通过CXCL12/CXCR4信号轴抑制口腔鳞癌细胞迁移及侵袭能力

金松1,田立华1*,苏勤1,郭艳2   

  1. 1. 华中科技大学同济医学院附属普爱医院口腔科 湖北 武汉 430033;
    2. 中国医科大学第四医院口腔科 辽宁 沈阳 110000
  • 收稿日期:2016-10-26 出版日期:2017-05-20 发布日期:2017-05-26
  • 通讯作者: 田立华,电话:13247238496
  • 作者简介:金松,男(1978~ ),学士,主治医师,主要从事口腔修复与种植的临床治疗工作。
  • 基金资助:
    辽宁省自然科学基金(编号:2013021094)

IL-24 Might Inhibit Migration and Invasion Abilities of Oral Squamous Cell Carcinoma Cells through CXCL12/CXCR4 Signal Axis.

JIN Song1, TIAN Li-hua1*, SU Qin1, GUO Yan2.   

  1. 1. Department of Stomatology, Puai Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430033, China; 2. Department of Stomatology, Fourth Hospital of China Medical University, Shenyang 110000, China.
  • Received:2016-10-26 Online:2017-05-20 Published:2017-05-26

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摘要： 目的:探讨慢病毒介导IL-24基因转染口腔鳞癌细胞对细胞迁移和侵袭能力的影响及对CXCL12/CXCR4信号轴的影响。方法:培养OSCC细胞株,分为联合处理组、IL-24转染组、抑制剂组和空白对照组。转染后培养48 h,ELISA法检测各组细胞液中IL-24表达,检测不同处理组细胞细胞凋亡、迁移和侵袭能力,实时荧光定量PCR和Western blot法检测细胞中IL-24、CXCL12和CXCR4基因和蛋白表达。结果:联合处理组和IL-24转染组细胞上清液中IL-24水平均显著高于抑制剂组和空白对照组(P<0.05);联合处理组迁移数和侵袭数均低于IL-24转染组、抑制剂组和空白对照组,且IL-24转染组和抑制剂组均低于空白对照组(P<0.05);联合处理组和IL-24转染组细胞中IL-24 基因和蛋白表达量均高于抑制剂组和空白对照组(P<0.05),联合处理组和IL-24转染组细胞中CXCL12和CXCR4 基因和蛋白表达量均低于抑制剂组和空白对照组(P<0.05)。结论:上调IL-24可有效抑制OSCC细胞迁移及侵袭,可能与特异性抑制CXCL12/CXCR4信号通路有关。

关键词: 口腔鳞状细胞癌, IL-24, CXCL12/CXCR4信号轴, 迁移, 侵袭

Abstract: Objective: To investigate the effect of lentivirus-mediated IL-24 gene transfection on migration and invasion of oral squamous cell carcinoma (OSCC) cells and the effect on CXCL12/CXCR4 axis signal. Methods: OSCC cell lines were cultured and divided into combined treatment group, IL-24 transfected group, inhibitor group, and blank control group. After transfected culture for 48h, the levels of IL-24 in the cytosol were detected by using ELISA, and cells migration, invasion abilities, and apoptosis in different groups were detected. The expressions of IL-24, CXCL12, and CXCR4 genes and proteins in cells were detected by using real-time quantitative PCR and Western blot. Results: The levels of IL-24 in the supernatants of combined treatment group and IL-24 transfected group were significantly higher than those of inhibitor group and blank control group (P<0.05). The numbers of cell migration and invasion in the combined treatment group were less than those in the IL-24 transfected group, inhibitor group, and blank control group, and those in the IL-24 transfected group and inhibitor group were less than those in the blank control group (P<0.05). The expression levels of IL-24 mRNA and proteins in the combined treatment group and IL-24 transfected group were higher than those in the inhibitor group and blank control group (P<0.05). The expression levels of CXCL12 and CXCR4 genes and proteins in the combined treatment group and IL-24 transfected group were lower than those in the inhibitor group and blank control group (P<0.05). Conclusion: Up-regulation of IL-24 could inhibit cell migration and invasion of OSCC. It



基金项目 辽宁省自然科学基金(编号:2013021094)
作者简介 金松,男(1978~ ),学士,主治医师,主要从事口腔修复与种植的临床治疗工作。
*通讯作者 田立华,电话:13247238496
might relate with the specific inhibiting CXCL12/CXCR4 signaling pathway.

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