脂肪干细胞的培养鉴定及多向诱导分化

doi:10.13701/j.cnki.kqyxyj.2017.08.002

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (8): 807-810.DOI: 10.13701/j.cnki.kqyxyj.2017.08.002

脂肪干细胞的培养鉴定及多向诱导分化

王琳¹, 吴国民^1*, 孙秀梅², 肖艳菊¹, 陈凯¹, 彭培轩¹

1. 吉林大学口腔医院口腔颌面外科吉林长春 130021;

2. 吉林大学口腔医院正畸科吉林长春 130021

收稿日期:2017-02-15 出版日期:2017-08-20 发布日期:2017-08-28
通讯作者: 吴国民,电话:0431-85579326
作者简介:王琳(1987~ ),女,吉林通化人,硕士,医师,主要从事口腔颌面外科、颌骨整形研究。
基金资助:
国家自然科学青年基金(编号:81400532);吉林省卫生计生青年科研课题(编号:2015q017);2015年度吉林大学白求恩培育计划(编号:2015301);吉林大学优秀青年教师培养计划(编号:419080500367)

Cultivation, Identification and Multiple Differentiation of Rabbit Adipose-derived Stem Cells.

WANG Lin¹, WU Guo-min^1*, SUN Xiu-mei², XIAO Yan-ju¹, CHEN Kai¹, PENG Pei-xuan¹.

1. Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Jilin University, Changchun 130021, China;
2. Department of Orthodontics, School and Hospital of Stomatology, Jilin University, Changchun 130021, China.

Received:2017-02-15 Online:2017-08-20 Published:2017-08-28

摘要/Abstract

摘要： 目的:进行脂肪干细胞的分离、培养和鉴定,阐明脂肪干细胞的生物学特性。方法:无菌条件下取新西兰大耳白兔腹股沟处脂肪组织,从中获得纯化的脂肪来源干细胞。HE染色观察细胞形态;流式细胞术检测细胞表面标志物;多向诱导后检测其成脂、成骨及成软骨分化能力。结果:提取的兔脂肪干细胞形态为成纤维细胞样,生长旺盛,可以稳定增殖20代以上。流式细胞术分析显示CD44呈阳性表达,CD34、CD14呈阴性表达;经体外诱导培养后,向成脂细胞、成骨细胞及成软骨细胞分化。结论:本实验成功分离、培养和鉴定了脂肪干细胞,脂肪干细胞体外增殖稳定,并且具有成脂、成骨及成软骨多向分化潜能。

关键词: 脂肪间充质干细胞, 多向分化

Abstract: Objective: To isolate, cultivate, identify, and multipotential differentiate the rabbit adipose-derived stem cells. Methods: Adipose tissue was taken from New Zealand white rabbits groin and the cells were obtained. The cells were cultured and observed. Phenotypic markers and differentiation characteristics of cells were evaluated. Results: Cells obtained were kind of fibroblast-like cells which grew rapidly. The cells could proliferate stably up to 20 passages or more. The cell surface antigens CD34 and CD14 were negative, while CD44 were positive. In addition, they could differentiate into osteoblasts, adipocytes, and chondrocytes. Conclusion: ADSCs were isolated successfully. The cells proliferated in vitro steadily and had good multiple differentiation potential.

Key words: Adipose-derived stem cells , Multiple differentiation

中图分类号:

R318.08

王琳, 吴国民, 孙秀梅, 肖艳菊, 陈凯, 彭培轩. 脂肪干细胞的培养鉴定及多向诱导分化[J]. 口腔医学研究, 2017, 33(8): 807-810.

WANG Lin, WU Guo-min, SUN Xiu-mei, XIAO Yan-ju, CHEN Kai, PENG Pei-xuan.. Cultivation, Identification and Multiple Differentiation of Rabbit Adipose-derived Stem Cells.[J]. Journal of Oral Science Research, 2017, 33(8): 807-810.

参考文献

[1] Huang JI, Zuk PA, Jones NF, et al. Chondrogenic potential of multipotential cells from human adipose tissue [J]. Plast Reconstr Surg, 2004, 113(5)∶585-594
[2] Mirsaidi A, Kleinhans K.N, Rimann M, et al. Telomere length, telomerase activity and osteogenic differentiation are maintained in adipose-derived stromal cells from senile osteoporotic SAMP6 mice [J]. Tissue Eng. Regen. Med, 2012, 6(3)∶378-390
[3] 李龙,赵建辉,刘斌,等.兔脂肪干细胞的分离培养鉴定及诱导分化研究[J].口腔医学研究,2012,28(5)∶426-429
[4] 李瑞妮,荣为为,金世柱,等.骨髓间充质干细胞体外增殖的策略[J].医学研究生学报 2015,28(5)∶549-552
[5] 孙萌, 段维勋, 刘金成, 等.心脏干细胞研究进展与临床应用[J].中国体外循环杂志 2015,13(3)∶189-192
[6] 张洪雨,刘志鑫,李兆鹏, 等.肌源干细胞的研究进展[J].医学临床研究 2015,32(1)∶191-193
[7] 李芳菲,刘娜,杨曦,等.脂肪来源干细胞膜片与支持材料结合能力的初步探讨[J].口腔医学研究 2015,31(10)∶969-973
[8] 周紫微,赵宇.脂肪干细胞研究进展与应用前景[J].安徽医科大学学报,2013,48(7)∶846-849
[9] 李传将,王万明.兔脂肪组织来源千细胞体外生长特性及培养的适宜条件[J].中国组织工程研究与临床康复,2009,13(19)∶3685-3688
[10] Choudhery MS, Badowski M, Muise A, et al. Cryopreservation of whole adipose tissue for future use in regenerative medicine [J]. Surg Res, 2014, 187(1)∶24-35
[11] 陈犹白,陈聪慧,Qixu Zhang,等.脂肪干细胞分离、纯化和保存:研究进展与未来方向[J].中国组织工程研究,2016,20(10)∶1508-1520
[12] Busilacchi A, GiganteA, Mattioli-Belmonte M, et al. Chitosan stabilizes platelet growth factors and modulates stem cell differentiation toward tissue regeneration [J]. Carbohydr Polym,2013,98(1)∶665-676
[13] De Ugarte D A, Morizono K, Elbarbary A, et al. Cornparison of muftilineage ceils from human adipose tissue and bone marrow [J]. Cells Tissues Organs, 2003, 174(3)∶101-109
[14] 许海委,徐宝山,杨强,等.不同方法诱导兔脂肪干细胞向软骨细胞分化的对比研究[J].中华创伤杂志,2014,30(12)∶1219-1223
[15] Stem Straeter J, Bonaterra GA,Juritz S,et al. Evaluation of the effects of different culture media on the myogenic differentiation potential of adipose tissue-Or bone marrow-derived human mesenchymal stem cells [J]. International joumalof molecular medicine,2014,33(1)∶160-170
[16] 赵娜.脂肪干细胞诱导分化的现状及前景[J].中国组织工程研究,2015,19(6)∶969-974

脂肪干细胞的培养鉴定及多向诱导分化

Cultivation, Identification and Multiple Differentiation of Rabbit Adipose-derived Stem Cells.

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