微小RNA-218-5p靶向三磷酸腺苷结合盒超家族G家族第2个成员基因对人口腔鳞状细胞癌细胞增殖、迁移和侵袭的影响

doi:10.13701/j.cnki.kqyxyj.2019.10.018

口腔医学研究 ›› 2019, Vol. 35 ›› Issue (10): 984-988.DOI: 10.13701/j.cnki.kqyxyj.2019.10.018

微小RNA-218-5p靶向三磷酸腺苷结合盒超家族G家族第2个成员基因对人口腔鳞状细胞癌细胞增殖、迁移和侵袭的影响

魏薇, 唐祎^*, 代婧, 陈胡杰

湖北省荆州市中心医院口腔科湖北荆州 434000

收稿日期:2019-01-09 出版日期:2019-10-28 发布日期:2019-10-22
通讯作者: 唐祎,E-mail:20984688@qq.com
作者简介:魏薇(1981~ )女,汉族,湖北荆州人,学士,主治医师,研究方向:牙周病。
基金资助:
湖北省卫生计划项目(编号:WJ2017M242)

Effects of MicroRNA-218-5p Targeting ABCG2 Gene on Proliferation, Migration, and Invasion of Tca-8113 Cells.

WEI Wei, TANG Yi^*, DAI Jing, CHEN Hujie

Department of Stomatology, Jingzhou Central Hospital, Jingzhou 434000, China.

Received:2019-01-09 Online:2019-10-28 Published:2019-10-22

摘要/Abstract

摘要： 目的:研究微小RNA(microRNA,miR)-218-5p靶向调控三磷酸腺苷结合盒超家族G家族第2个成员(ATP-binding cassette sub-family G member 2,ABCG2)基因对Tca-8113细胞的增殖、迁移和侵袭的影响,并探讨其机制。方法:运用实时荧光定量聚合酶链反应(quantitative Real-time PCR, qRT-PCR)与Western blot检测Tca-8113细胞和NOK细胞中miR-218-5p和ABCG2的表达。通过双荧光素酶报告基因实验检测miR-218-5p和ABCG2靶向关系。用脂质体法将miR-218-5p mimic、ABCG2 小干扰RNA(interfering RNA, siRNA)、miR-218-5p mimic+pcDNA-ABCG2分别转染至Tca-8113细胞中,噻唑蓝( methyl thiazol tetrazolium,MTT)法、Transwell法检测Tca-8113细胞的增殖、迁移和侵袭。结果:与NOK细胞比较,Tca-8113细胞miR-218-5p呈显著低表达,ABCG2呈显著高表达(P<0.05)。过表达miR-218-5p、抑制ABCG2均可抑制Tca-8113细胞的增殖和迁移(P<0.05)。双荧光素酶报告基因实验证实miR-218-5p与ABCG2的靶向结合关系。过表达ABCG2可逆转上调miR-218-5p对Tca-8113细胞增殖、迁移和侵袭的抑制作用。结论:上调miR-218-5p的表达可靶向调控ABCG2,抑制Tca-8113细胞的增殖、迁移和侵袭,将可为口腔鳞状细胞癌的治疗提供新的靶点。

关键词: 口腔鳞状细胞癌, 微小RNA-218-5p, 三磷酸腺苷结合盒超家族G家族第2个成员, 增殖, 迁移, 侵袭

Abstract: Objective: To investigate the effects of miR-218-5p targeting ABCG2 gene on the proliferation, migration, and invasion of Tca-8113 cells and its mechanism. Methods: qRT-PCR and western blot were used to detect the expression of miR-218-5p and ABCG2 in Tca-8113 and NOK cells. The targeting relationship between miR-218-5p and ABCG2 was detected by dual luciferase reporter gene experiment. miR-218-5p mimic, ABCG2 siRNA and miR-218-5p mimic+pcDNA-ABCG2 were transfected into Tca-8113 cells by liposome method. The proliferation, migration, and invasion of Tca-8113 cells were detected by MTT and Transwell tests. Results: Compared with NOK cells, miR-218-5p was significantly down-regulated in Tca-8113 cells, and ABCG2 was significantly expressed (P<0.05). Over-expression of miR-218-5p and inhibition of ABCG2 inhibited proliferation and migration of Tca-8113 cells (P<0.05). There is a targeted binding relationship between miR-218-5p and ABCG2. Over-expression of ABCG2 reverses the inhibitory effect of up-regulated miR-218-5p on proliferation, migration, and invasion of Tca-8113 cells. Conclusion: Up-regulating the expression of miR-218-5p can target ABCG2 to inhibit the proliferation, migration, and invasion of Tca-8113 cells, which may provide a new target for the treatment of OSCC.

Key words: oral squamous cell carcinoma, microRNA-218-5p, ATP-binding cassette sub-family G member 2, proliferation, migration, invasion

魏薇, 唐祎, 代婧, 陈胡杰. 微小RNA-218-5p靶向三磷酸腺苷结合盒超家族G家族第2个成员基因对人口腔鳞状细胞癌细胞增殖、迁移和侵袭的影响[J]. 口腔医学研究, 2019, 35(10): 984-988.

WEI Wei, TANG Yi, DAI Jing, CHEN Hujie. Effects of MicroRNA-218-5p Targeting ABCG2 Gene on Proliferation, Migration, and Invasion of Tca-8113 Cells.[J]. Journal of Oral Science Research, 2019, 35(10): 984-988.

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微小RNA-218-5p靶向三磷酸腺苷结合盒超家族G家族第2个成员基因对人口腔鳞状细胞癌细胞增殖、迁移和侵袭的影响

Effects of MicroRNA-218-5p Targeting ABCG2 Gene on Proliferation, Migration, and Invasion of Tca-8113 Cells.

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