Fas配体过表达对人牙周膜干细胞成骨分化的影响

doi:10.13701/j.cnki.kqyxyj.2019.12.017

口腔医学研究 ›› 2019, Vol. 35 ›› Issue (12): 1169-1172.DOI: 10.13701/j.cnki.kqyxyj.2019.12.017

Fas配体过表达对人牙周膜干细胞成骨分化的影响

马灵芝¹, 任娅岚¹, 钱丽萍¹, 戈文斌¹, 刘亚丽¹, 周治^2*

1. 昆明医科大学口腔医学院云南昆明 650500;
2. 云南省第二人民医院口腔正畸科云南昆明 650021

收稿日期:2019-06-10 出版日期:2019-12-28 发布日期:2019-12-23
通讯作者: 周治,E-mail: km-ha@163.com
作者简介:马灵芝(1995~ ),女,陕西宝鸡人,硕士在读,主要从事口腔临床医学基础研究工作。
基金资助:
国家自然科学基金(编号:31301067、31860326)云南省应用基础研究计划项目(编号:2017FE468-227、2018FE001-260)昆明医科大学研究生创新基金(编号:2019S018)

Effects of Fas Ligand Overexpression on Osteogenic Differentiation Potential of Human PDLSCs

MA Lingzhi¹, REN Yalan¹, QIAN Liping¹, GE Wenbin¹, LIU Yali¹, ZHOU Zhi^2*

1. School of Stomatology, Kunming Medical University, Kunming 650500, China;
2. Department of Orthodontics, The Second People's Hospital of Yunnan Province, Kunming 650021, China.

Received:2019-06-10 Online:2019-12-28 Published:2019-12-23

摘要/Abstract

摘要： 目的:探讨Fas配体(Fas ligand,FasL)过表达对人牙周膜干细胞(periodontal ligament stem cells,PDLSCs)骨向分化能力的影响。方法:体外有限稀释克隆化培养人PDLSCs,构建FasL过表达质粒,转染至PDLSCs,实验分为对照组(Control 组)、空载体对照组(NC组)、FasL过表达组(EX组),对各组进行成骨诱导,茜素红染色、实时荧光定量PCR(Real-time PCR)和Western blot检测PDLSCs成骨分化的能力。结果:EX组成骨分化能力较Control组和NC组有明显升高(P＜0.05),Control组和NC组比较差异无统计学意义(P＞0.05)。 结论:FasL可以促进PDLSCs成骨分化。

关键词: Fas配体, 牙周膜干细胞, 骨向分化

Abstract: Objective: To investigate the effect of FasL overexpression on osteogenic differentiation of human periodontal ligament stem cells (PDLSCs). Methods: Human PDLSCs were cloned and cultured in vitro with limited dilution. FasL overexpression plasmid was constructed and transfected into PDLSCs. The experiment was divided into control group, no-load control group (NC group), and FasL overexpression group (EX group). Osteogenic was induced in each group. Alizarin red staining, real-time PCR, and western blot were used to detect the osteogenic differentiation ability of PDLSCs. Results: The osteogenic differentiation ability of EX group was significantly higher than that of control group and NC group (P<0.05). There was no significant difference between control group and NC group (P>0.05). Conclusion: FasL can promote the osteogenic differentiation of human PDLSCs.

Key words: FasL, periodontal ligament stem cells, osteogenic differentiation potential

马灵芝, 任娅岚, 钱丽萍, 戈文斌, 刘亚丽, 周治. Fas配体过表达对人牙周膜干细胞成骨分化的影响[J]. 口腔医学研究, 2019, 35(12): 1169-1172.

MA Lingzhi, REN Yalan, QIAN Liping, GE Wenbin, LIU Yali, ZHOU Zhi. Effects of Fas Ligand Overexpression on Osteogenic Differentiation Potential of Human PDLSCs[J]. Journal of Oral Science Research, 2019, 35(12): 1169-1172.

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Fas配体过表达对人牙周膜干细胞成骨分化的影响

Effects of Fas Ligand Overexpression on Osteogenic Differentiation Potential of Human PDLSCs

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