白花丹素通过ROS介导的JNK通路诱导舌鳞状细胞癌细胞凋亡

doi:10.13701/j.cnki.kqyxyj.2020.10.008

口腔医学研究 ›› 2020, Vol. 36 ›› Issue (10): 921-924.DOI: 10.13701/j.cnki.kqyxyj.2020.10.008

白花丹素通过ROS介导的JNK通路诱导舌鳞状细胞癌细胞凋亡

周群, 邱嘉旋^*

南昌大学第一附属医院口腔颌面外科江西南昌 330006

收稿日期:2020-03-06 出版日期:2020-10-28 发布日期:2020-10-19
通讯作者: *邱嘉旋,E-mail:qiujiaxuan@163.com
作者简介:周群(1994~ ),女,河南信阳人,硕士,主要从事口腔颌面部肿瘤研究。
基金资助:
国家自然科学基金(编号:81860477);江西省重点研发计划(编号:20181ACG70009)

Plumbagin Induces Tongue Squamous Cell Carcinoma Cells Apoptosis via ROS-mediated JNK Signaling Pathway

ZHOU Qun, QIU Jiaxuan^*

Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital of Nanchang University, Nanchang 330006, China

Received:2020-03-06 Online:2020-10-28 Published:2020-10-19

摘要/Abstract

摘要： 目的: 探究白花丹素诱导舌鳞状细胞癌细胞凋亡的分子机制。方法: CCK-8实验检测白花丹素对舌鳞状细胞癌细胞增殖的影响,流式细胞术检测舌鳞状细胞癌细胞凋亡率和细胞内活性氧水平,Western blot 检测Bcl-2、Bax和p-JNK蛋白的表达。 结果: CCK-8显示白花丹素有显著的抗舌鳞状细胞癌细胞增殖作用(P<0.001)。流式细胞术显示白花丹素可呈剂量依赖性地促进舌鳞状细胞癌细胞凋亡(P＜0.001),有效刺激舌鳞状细胞癌细胞内产生活性氧(P＜0.001)。Western blot结果显示,白花丹素作用后Bax/Bcl-2的比值升高(P<0.01,P<0.001),p-JNK蛋白表达随着作用时间延长逐渐升高(P＜0.05,P＜0.01,P＜0.001)。然而,当我们使用活性氧清除剂NAC后,p-JNK蛋白表达和细胞凋亡率都被显著逆转(P<0.001,P<0.001)。 结论: 白花丹素可以有效抑制舌鳞状细胞癌细胞的增殖,此外白花丹素可以通过活性氧介导的JNK途径诱导舌鳞状细胞癌细胞凋亡。

关键词: 白花丹素, 舌鳞状细胞癌, 细胞凋亡, 活性氧

Abstract: Objective: To explore the underlying molecular mechanisms of plumbagin-induced apoptosis of tongue squamous cell carcinoma cells. Methods: The effect of plumbagin on cell proliferation was determined by CCK-8 assay. Cell apoptosis and the expression of cellular ROS generation were measured by flow cytometry. Protein levels of Bcl-2, Bax, and p-JNK were evaluated by Western blot. Results: CCK-8 assay showed that plumbagin significantly inhibited cell proliferation in a dose-dependent manner(P<0.001). The results of flow cytometer suggested that plumbagin markedly increased cell apoptosis and ROS generation. Western blot showed that the expression of Bax increased, while the expression of Bcl-2 decreased after plumbagin treatment. In a time-dependent manner, plumbagin up-regulated the expressions of p-JNK (P<0.05,P<0.01,P<0.001). However, the ROS inhibitor NAC significantly reversed the expression level of p-JNK and cell apoptosis(P<0.001,P<0.001). Conclusion: Plumbagin had excellent anti-proliferation effects on tongue squamous cell carcinoma cells. In addition, plumbagin induced cell apoptosis through ROS-mediated JNK pathway.

Key words: plumbagin, tongue squamous cell carcinoma, cell apoptosis, reactive oxygen species

周群, 邱嘉旋. 白花丹素通过ROS介导的JNK通路诱导舌鳞状细胞癌细胞凋亡[J]. 口腔医学研究, 2020, 36(10): 921-924.

ZHOU Qun, QIU Jiaxuan. Plumbagin Induces Tongue Squamous Cell Carcinoma Cells Apoptosis via ROS-mediated JNK Signaling Pathway[J]. Journal of Oral Science Research, 2020, 36(10): 921-924.

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白花丹素通过ROS介导的JNK通路诱导舌鳞状细胞癌细胞凋亡

Plumbagin Induces Tongue Squamous Cell Carcinoma Cells Apoptosis via ROS-mediated JNK Signaling Pathway

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