纳米弱晶态生物煅烧骨修复材料的制备及其性能研究

doi:10.13701/j.cnki.kqyxyj.2024.01.014

口腔医学研究 ›› 2024, Vol. 40 ›› Issue (1): 73-79.DOI: 10.13701/j.cnki.kqyxyj.2024.01.014

纳米弱晶态生物煅烧骨修复材料的制备及其性能研究

祝超¹, 张铁¹, 张旗¹, 胡丽¹, 邱迪², 张利攀¹, 马晓丹^2*, 吴猛³, 李小雅¹, 黄爱军⁴

1.武汉英领医学生物科技有限公司湖北武汉430206;
2.湖北工业大学湖北武汉 430068;
3.武汉大学中南医院超声科湖北武汉 430071;
4.中山大学附属第八医院深圳福田 518033

收稿日期:2023-07-11 出版日期:2024-01-28 发布日期:2024-01-22
通讯作者: ^*马晓丹,E-mail:20180025@hbut.edu.cn
作者简介:祝超(1991~ )女,湖北人,硕士,主要从事生物材料医疗器械研究工作。

Preparation and Properties of Nano Low-crystalline Bio-Calcined Bone Repair Material

ZHU Chao¹, ZHANG Tie¹, ZHANG Qi¹, HU Li¹, QIU Di², ZHANG Lipan¹, MA Xiaodan^2*, WU Meng³, LI Xiaoya¹, HUANG Aijun⁴

1. Leader Med-Biotech. Inc. Wuhan 430206, China;
2. Hubei University of Technology, Wuhan 430068, China;
3. Department of Ultrasound, Zhongnan Hospital, Wuhan University, Wuhan 430071, China;
4. The Eighth Affiliated Hospital, Sun Yat Sen University, Shenzhen 518033, China

Received:2023-07-11 Online:2024-01-28 Published:2024-01-22

摘要/Abstract

摘要： 目的:通过制备一种纳米弱晶态生物煅烧骨修复材料,与国内同类产品对比,研究材料的微观结构对成骨性能的影响,探讨用于临床的可行性。方法:通过脱脂、去蛋白以及煅烧等工序制备煅烧骨修复材料,并研究材料的成分、结构、生物相容性;通过与市场上的天然煅烧骨修复材料进行对比,研究其二者理化性能的差异,以及差异对成骨性能和安全性的影响。结果:制备的生物煅烧骨修复材料和天然煅烧骨修复材料的主要成分均为弱结晶态的羟基磷灰石,晶体尺寸均为纳米级,孔径范围和分布基本一致,生物煅烧骨修复材料的比表面积为91.96 m²/g,长针状晶型,天然煅烧骨修复材料的比表面积为52.51 m²/g,圆形晶型,生物煅烧骨修复材料较天然煅烧骨修复材料保留了更多的碳酸盐;生物学试验结果显示生物煅烧骨修复材料具有良好的生物相容性,动物试验结果显示生物煅烧骨修复材料和天然煅烧骨修复材料都能够有效抑制拔牙后的牙槽骨萎缩以及促进骨组织的修复,二者无统计学差异。结论:生物煅烧骨修复材料为一种纳米弱晶态的骨修复材料,保留骨组织无机盐的成分和结构,具有良好的生物相容性,在比格犬动物试验中,能够有效抑制牙槽骨萎缩,促进骨组织修复,有望用于临床。

关键词: 煅烧骨, 弱晶态, 纳米, 羟基磷灰石, 牙槽骨

Abstract: Objective: To study the effect of microstructure on osteogenesis property of nano low-crystalline bio-calcined bone repair material. Methods: The bio-calcined bone repair material was prepared by degreasing, deproteinizing and calcination, and its composition, structure, and biocompatibility were studied. The physical and chemical properties of the bio-calcined bone repair material were characterized, and then compared with those of natural calcined bovine bone. The influence of the differences on the osteogenic properties and safety were studied. Results: The main components of bio-calcined bone repair material and natural calcined bovine bone were low-crystalline hydroxyapatite, whose crystal sizes were all nanometer and whose pore size range and distribution were the same. The internal surface area of the bio-calcined bone repair material was 91.96 m²/g, and the bio-calcined bone repair material had acicular crystal. The internal surface area of natural calcined bovine bone was 52.51 m²/g, and natural calcined bovine bone had round crystal. The bio-calcined bone repair material retained more carbonate than natural calcined bovine bone. The biological test results showed that the bio-calcined bone repair material had good biocompatibility. The animal test results showed that both bio-calcined bone repair material and natural calcined bovine bone could effectively inhibit the alveolar bone atrophy and promote the repair of bone tissue after extraction, and there was no statistical difference between two materials. Conclusion: Bio-calcined bone repair material is a kind of nano low-crystalline bone repair material, which retains the composition and structure of inorganic salt of bone tissue, and has good biocompatibility. It is expected to be used in clinical practice.

Key words: calcined bone, low-crystalline, nanometer, hydroxyapatite, alveolar bone

祝超, 张铁, 张旗, 胡丽, 邱迪, 张利攀, 马晓丹, 吴猛, 李小雅, 黄爱军. 纳米弱晶态生物煅烧骨修复材料的制备及其性能研究[J]. 口腔医学研究, 2024, 40(1): 73-79.

ZHU Chao, ZHANG Tie, ZHANG Qi, HU Li, QIU Di, ZHANG Lipan, MA Xiaodan, WU Meng, LI Xiaoya, HUANG Aijun. Preparation and Properties of Nano Low-crystalline Bio-Calcined Bone Repair Material[J]. Journal of Oral Science Research, 2024, 40(1): 73-79.

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纳米弱晶态生物煅烧骨修复材料的制备及其性能研究

Preparation and Properties of Nano Low-crystalline Bio-Calcined Bone Repair Material

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