口腔医学研究

口腔医学研究 ›› 2026, Vol. 42 ›› Issue (8): 696-701.DOI: 10.13701/j.cnki.kqyxyj.2026.08.010

• 口腔材料学研究 • 上一篇    下一篇

纳米管结构钛表面调控成骨细胞线粒体功能以增强细胞代谢

武钺1, 贺扬帆2*   

  1. 1.昆明市口腔医院口腔内科 云南 昆明 650034;
    2.昆明市口腔医院口腔种植科 云南 昆明 650034
  • 收稿日期:2025-11-18 出版日期:2026-08-28 发布日期:2026-08-17
  • 通讯作者: *贺扬帆,E-mail:3942782120@qq.com
  • 作者简介:武钺(1972~ ),女,云南人,硕士,副主任医师,研究方向:种植体表面改性。

Nanotopographical Titanium Surfaces Promote Cellular Bioenergetics through Mitochondrial Modulation

WU Yue1, HE Yangfan2*   

  1. 1. Department of Endodontology, Kunming Municipal Stomatological Hospital, Kunming 650034, China;
    2. Department of Implantology, Kunming Municipal Stomatological Hospital, Kunming 650034, China
  • Received:2025-11-18 Online:2026-08-28 Published:2026-08-17

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摘要： 目的: 探讨纳米管结构钛表面对成骨前体细胞线粒体功能及代谢活性的调控作用。方法: 通过阳极氧化在钛表面制备直径约100 nm的纳米管结构,采用扫描电子显微镜观察表面形貌;检测细胞代谢活性、黏附及形态;共聚焦显微镜分析线粒体网络,并通过实时荧光定量聚合酶链反应(quantitative real-time polymerase chain reaction,qRT-PCR)检测线粒体相关基因表达。结果: 钛表面纳米管排列均匀,其表面细胞第1天代谢活性无显著差异,第7天显著增强。细胞在未改性钛表面呈梭形伸长,在纳米管结构表面不规则铺展。线粒体在细胞内的均匀分布,纳米结构降低网络分支数,但不影响面积及分支长度;过氧化物酶体增殖物激活受体γ共激活因子1-α(peroxisome proliferator-activated receptor gamma coactivator 1-alpha,Pgc-1α)在第1天上调约24倍,其它基因无明显变化。此外,Pgc-1α蛋白表达也不受纳米管结构钛表面影响。结论: 纳米管结构钛表面通过调控线粒体网络和能量代谢,增强细胞代谢活性。Pgc-1α早期上调提示可能激活线粒体生物发生信号,改善能量状态并促进成骨潜能,为钛种植体表面设计提供理论依据。

关键词: 钛表面改性, 纳米管结构, 线粒体功能, 细胞代谢, 基因表达

Abstract: Objective: To investigate the effects of nanotopographical titanium surfaces on mitochondrial function and metabolic activity in pre-osteoblasts. Methods: Nanotubular structures (~100 nm) were fabricated by anodization. Surface morphology, cell metabolic activity, adhesion, and morphology were assessed. Mitochondrial network was analyzed by confocal microscopy, and expression of mitochondrial-related genes was measured by RT-PCR. Results: Nanotubular surfaces were uniform. Metabolic activity was similar on day 1 but increased on day 7. Cells spread irregularly on nanotopographical surfaces versus elongated on unmodified titanium. Mitochondria were evenly distributed, and nanotopography reduced branch number without affecting area or branch length. Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (Pgc-1α) was upregulated ~24-fold on day 1; however, other genes remained unchanged. Furthermore, Pgc-1α protein expression was not affected by titanium nanotube structure. Conclusion: Nanotopographical titanium enhances cell metabolism by modulating mitochondrial network and bioenergetics. Early Pgc-1α upregulation suggests mitochondrial biogenesis activation, improving energy status and osteogenic potential, providing insights for implant surface design.

Key words: titanium surface modification, nanotopography, mitochondrial function, cellular metabolism, gene expression