负载镁离子水凝胶的制备及其调控小鼠单核-巨噬细胞系RAW264.7细胞极化性能研究

doi:10.13701/j.cnki.kqyxyj.2026.08.009

口腔医学研究 ›› 2026, Vol. 42 ›› Issue (8): 688-695.DOI: 10.13701/j.cnki.kqyxyj.2026.08.009

负载镁离子水凝胶的制备及其调控小鼠单核-巨噬细胞系RAW264.7细胞极化性能研究

刘阳¹, 杨吉垒^2#, 李晓雨³, 王文丽¹, 左孝轩¹, 李友瑞^1*, 宋花蕾^4*

1.山东医药大学附属医院口腔修复科山东滨州 256603;
2.德州市中医院口腔科山东德州 253000;
3.齐鲁医药学院山东淄博 255213;
4.山东医药大学附属医院医学遗传中心山东滨州 256603

收稿日期:2025-11-13 出版日期:2026-08-28 发布日期:2026-08-17
通讯作者: ^*李友瑞,E-mail:carylee37@qq.com; 宋花蕾,E-mail:shl20080701@163.com
作者简介:刘阳(1999~ ),男,河南人,硕士,住院医师,研究方向:骨组织工程。杨吉垒(1984~ ),男,山东禹城人,副主任医师,研究方向:骨组织工程与口腔种植。#为共同第一作者
基金资助:
山东省自然科学基金面上项目(编号:ZR2023MH039); 德州市重点研发计划(医教体科技专项)(2024~2026年); 德州市中医药学会中医药科技创新计划项目(编号:DZZY2023015)

Preparation of Magnesium Ion-loaded Hydrogel and Study on Its Regulating Polarization Performance of RAW264.7 Cells

LIU Yang¹, YANG Jilei^2#, LI Xiaoyu³, WANG Wenli¹, ZUO Xiaoxuan¹, LI Yourui^1*, SONG Hualei^4*

1. Department of Prosthodontics, Affiliated Hospital of Shandong Medical University, Binzhou 256603, China;
2. Department of Stomatology, Dezhou Hospital of Traditional Chinese Medicine, Dezhou 253000, China;
3. Qilu Medical University, Zibo 255213, China;
4. Medical Genetics Center, Affiliated Hospital of Shandong Medical University, Binzhou 256603, China

Received:2025-11-13 Online:2026-08-28 Published:2026-08-17

摘要/Abstract

摘要： 目的: 探讨负载不同浓度镁离子水凝胶支架理化性能及其对小鼠单核-巨噬细胞白血病细胞系RAW264.7细胞增殖与极化的影响。方法: 利用物理共混法将甲基丙烯酰化明胶(methylpropenylated gelatin,GeLMA)与不同质量的氯化镁(MgCl₂)制备成负载不同质量百分比镁离子的复合水凝胶支架,并通过扫描电镜、能谱仪分析、傅里叶红外光谱等进行表征分析及降解缓释性能检测。通过活/死细胞染色、CCK-8、细胞骨架染色检测支架生物相容性;免疫荧光染色及实时荧光定量聚合酶链反应(quantitative real-time polymerase chain reaction,qRT-PCR)检测各组支架对RAW264.7细胞极化的影响。结果: Mg²⁺成功负载于GeLMA中,并通过缓释促进RAW264.7向M2型极化。结论: GelMA/Mg²⁺水凝胶具备良好的生物相容性、亲水性能以及药物释放能力,能够促进RAW264.7向M2型极化。

关键词: 镁离子, 水凝胶, RAW264.7, 免疫调控, 成骨分化

Abstract: Objective: To investigate the physicochemical properties of hydrogel scaffolds loaded with different concentrations of magnesium ions and their effects on the proliferation and polarization of mouse monocytic macrophage leukemia cell line RAW264.7. Methods: Composite hydrogel scaffolds loaded with different mass percentages of magnesium ions were prepared by physically blending gelatin methacryloyl (GeLMA) with different masses of magnesium chloride (MgCl₂). The scaffolds were characterized by scanning electron microscopy, energy dispersive spectrometer analysis, Fourier transform infrared spectroscopy, etc., and their degradation and sustained release properties were tested. The biocompatibility of the scaffolds was evaluated by live/dead cell staining, CCK-8 assay, and cytoskeleton staining. The effects of each group of scaffolds on the polarization of RAW264.7 were detected by immunofluorescence staining and quantitative real-time polymerase chain reaction (qRT-PCR). Results: Magnesium ions were successfully loaded into GelMA and promoted the polarization of RAW264.7 to M2 type through sustained release. Conclusion: GelMA/Mg²⁺ hydrogels have good biocompatibility, hydrophilicity, and drug release ability, and can promote the polarization of RAW264.7 to M2 type.

Key words: magnesium ion, hydrogel, RAW264.7, immunoregulation, osteogenic differentiation

刘阳, 杨吉垒, 李晓雨, 王文丽, 左孝轩, 李友瑞, 宋花蕾. 负载镁离子水凝胶的制备及其调控小鼠单核-巨噬细胞系RAW264.7细胞极化性能研究[J]. 口腔医学研究, 2026, 42(8): 688-695.

LIU Yang, YANG Jilei, LI Xiaoyu, WANG Wenli, ZUO Xiaoxuan, LI Yourui, SONG Hualei. Preparation of Magnesium Ion-loaded Hydrogel and Study on Its Regulating Polarization Performance of RAW264.7 Cells[J]. Journal of Oral Science Research, 2026, 42(8): 688-695.

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负载镁离子水凝胶的制备及其调控小鼠单核-巨噬细胞系RAW264.7细胞极化性能研究

Preparation of Magnesium Ion-loaded Hydrogel and Study on Its Regulating Polarization Performance of RAW264.7 Cells

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